【摘要】：目的：通過模擬固定矯治后的牙釉質(zhì)表面脫礦,使用顯微硬度儀進行定量測量,并用掃描電鏡觀察釉質(zhì)表面形態(tài)結(jié)構(gòu),比較幾種再礦化產(chǎn)品對脫礦釉質(zhì)的再礦化效果,為其應(yīng)用提供實驗依據(jù)。 方法：選取因正畸治療而拔除的健康前磨牙90顆,隨機分為6組,每組15個樣本,分別進行不同的處理：A組：不作任何處理；B組：脫礦處理；C組：脫礦后浸泡在人工唾液中；D組：脫礦后浸泡在人工唾液中,牙齒頰面涂布氟保護漆；E組：脫礦后浸泡在人工唾液中,牙齒頰面涂布多樂氟；F：脫礦后浸泡在人工唾液中,牙齒頰面涂布GC護牙素。應(yīng)用顯微硬度儀測量所有樣本釉質(zhì)表面的顯微硬度值,應(yīng)用統(tǒng)計學軟件SPSS17.0對所測數(shù)據(jù)進行單因素方差分析,組間的兩兩比較用LSD-t檢驗,P0.05認為有統(tǒng)計學意義。另外從每組樣本中隨機挑選樣本在掃描電鏡下觀察釉質(zhì)表面形態(tài)結(jié)構(gòu)。 結(jié)果：①顯微硬度值結(jié)果比較A組的顯微硬度值最高,B組的顯微硬度值最低,D組、E組和F組的顯微硬度值均高于C組,F組的顯微硬度值高于D組和E組,以上差異均具有統(tǒng)計學意義(P=0.000,P0.05),D組和E組的顯微硬度值差異無統(tǒng)計學意義(P=0.355,P0.05)。②掃描電鏡觀察結(jié)果A組表現(xiàn)為正常釉質(zhì)表面形態(tài),B組呈為明顯的脫礦狀態(tài),釉柱溶解破壞呈蜂窩狀,C組、D組、E組和F組在釉質(zhì)表面均可見礦物質(zhì)沉積,其中C組礦物質(zhì)沉積較少,F組礦物質(zhì)沉積最明顯,D組和E組礦物質(zhì)沉積水平相當。 結(jié)論：①GC護牙素、多樂氟、0.1%氟保護漆和人工唾液均能使脫礦釉質(zhì)再礦化,使釉質(zhì)表面顯微硬度值增高,并能改善釉質(zhì)表面微觀形態(tài)。②GC護牙素、多樂氟、0.1%氟保護漆均能增強脫礦釉質(zhì)的再礦化效果,較單純使用人工唾液時的再礦化效果具有明顯優(yōu)勢,其中GC護牙素對顯微硬度的增高效果最為突出,其次為多樂氟和0.1%氟保護漆,多樂氟和0.1%氟保護漆對脫礦釉質(zhì)的再礦化效果尚未發(fā)現(xiàn)明顯差異。③正常牙釉質(zhì)的硬度最高,脫礦釉質(zhì)短時間內(nèi)經(jīng)再礦化處理也難以恢復至脫礦前釉質(zhì)硬度。
[Abstract]:Objective: to compare the demineralization effect of several remineralization products on enamel surface by imitating the demineralization of enamel surface after fixation, measuring quantitatively by microhardness instrument, observing the morphology of enamel surface by scanning electron microscope (SEM), and comparing the effect of several remineralization products on demineralization of enamel. To provide experimental basis for its application. Methods: 90 healthy premolars extracted by orthodontic treatment were randomly divided into 6 groups, 15 samples in each group were treated with different treatments: group B: demineralization; Group C: immerse in artificial saliva after demineralization, group D: after demineralization, immerse in artificial saliva, coated with fluorine protective paint E group: after demineralization, immerse in artificial saliva, and coat tooth buccal surface with Dorafur; F: soaked in artificial saliva after demineralization, and coated with GC dentin on the buccal surface of teeth. The microhardness of all samples was measured by microhardness instrument, and the data were analyzed by single factor ANOVA using statistical software SPSS17.0. The comparison between groups was considered statistically significant by LSD-t test (P0.05). In addition, samples were randomly selected from each group to observe the surface morphology of enamel under scanning electron microscope (SEM). Results the microhardness values of group A and group B were higher than those of group C and group F, respectively, and the values of group E and group F were higher than those of group C and group E. the microhardness values of group A were higher than those of group D and group E, and the values of group F were higher than those of group C and group E. There was no significant difference in microhardness between group D and group E (P < 0. 355P 0.05). 2 SEM observation showed that group A showed normal enamel surface morphology and group B showed obvious demineralization state, and there was no significant difference in microhardness between group D and group E (P < 0. 355P 0.05). The dissolution and destruction of enamel post were honeycombed in group C, group D, group E and group F, and mineral deposits were found on the surface of enamel. The mineral deposits in group C were less than those in group F, and the levels of mineral deposition in group D and group E were the same. Conclusion 1% GC dentin, 0.1% fluoride-protective paint and artificial saliva can make demineralized enamel mineralization, increase the microhardness of enamel surface, and improve the morphology of enamel surface .2GC. The effect of demineralization of demineralized enamel was enhanced by 0.1% fluoride-protective paint, which was superior to that of artificial saliva alone, and the effect of GC dentin on the increase of microhardness was the most obvious. The remineralization effect of Dorafur and 0.1% fluorine protective lacquer on demineralized enamel was not found to be significant difference. 3. The hardness of normal enamel was the highest. The enamel hardness of demineralized enamel before demineralization is difficult to recover after remineralization in a short time.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R783.5

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