發(fā)布時(shí)間：2018-09-14 10:50

【摘要】：目的 對(duì)比研究人牙髓干細(xì)胞(DPSCs)和根尖乳頭干細(xì)胞(SCAP)的體外生長(zhǎng)特性、增殖及礦化能力。方法 采用酶消化法體外培養(yǎng)人DPSCs和SCAP,誘導(dǎo)分化培養(yǎng)基誘導(dǎo)細(xì)胞成骨/成牙本質(zhì)向分化,流式細(xì)胞儀檢測(cè)特異性標(biāo)記物,茜素紅染色檢測(cè)礦化程度,逆轉(zhuǎn)錄PCR(RT-PCR)檢測(cè)分化標(biāo)記物表達(dá)。結(jié)果 人DPSCs和SCAP為成纖維細(xì)胞樣貼壁生長(zhǎng),SCAP增殖率較高;兩種細(xì)胞表達(dá)特異性間充質(zhì)干細(xì)胞(MSCs)標(biāo)記物:CD34、CD45(-),CD90、CD105、CD146(+),基質(zhì)細(xì)胞抗原1(STRO-1)、八聚體轉(zhuǎn)錄因子4(OCT-4)(+),SCAP特異性標(biāo)記物CD24(+)。成骨誘導(dǎo)3周可形成明顯鈣化結(jié)構(gòu),SCAP鈣化能力較強(qiáng)。成骨誘導(dǎo)2周2種細(xì)胞均可表達(dá)分化標(biāo)記物:骨涎蛋白、骨鈣素、牙本質(zhì)涎磷蛋白,且隨誘導(dǎo)時(shí)間表達(dá)逐漸增加。結(jié)論 人DPSCs和SCAP均具有間充質(zhì)干細(xì)胞典型特征,可分化為成牙本質(zhì)樣細(xì)胞,是牙源性組織工程的可靠干細(xì)胞來源。
[Abstract]:Objective to compare the growth characteristics, proliferation and mineralization of human dental pulp stem cells (DPSCs) and apical papilla stem cells (SCAP) in vitro. Methods Human DPSCs and SCAP, culture medium were used to induce osteoblast / dentin differentiation, flow cytometry was used to detect specific markers and alizarin red staining was used to detect mineralization. The expression of differentiation markers was detected by reverse transcription PCR (RT-PCR). Results the proliferation rate of human DPSCs and SCAP was higher than that of fibroblast-like adherent cells, and the two kinds of cells expressed specific mesenchymal stem cell (MSCs) markers: CD34-CD45 (-) CD90 CD105- CD146 (), stromal cell antigen 1 (STRO-1), OCT-4) () transcription factor 4 (OCT-4) () specific marker CD24 (). After 3 weeks of osteogenic induction, the calcified structure of SCAP was found to be highly calcified. Two weeks after osteogenic induction, two kinds of cells expressed differentiation markers: bone sialoprotein, osteocalcin, dentin sialophosphorus protein, and the expression increased gradually with the induction time. Conclusion both human DPSCs and SCAP have typical characteristics of mesenchymal stem cells and can differentiate into odontoblast cells, which is a reliable source of stem cells for odontogenic tissue engineering.
【作者單位】： 云南省第一人民醫(yī)院口腔內(nèi)科;昆明醫(yī)科大學(xué)第一附屬醫(yī)院心內(nèi)科;
【分類號(hào)】：R781.3

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