【摘要】：第一部分中國人數(shù)字恒牙數(shù)據(jù)庫的構建 目的：構建中國人恒牙形態(tài)數(shù)據(jù)庫,實現(xiàn)數(shù)據(jù)管理、查詢等功能。 方法：收集中國人不同牙位的離體牙,編號后使用Micro-CT掃描,掃描分辨率為30-37μm。通過VGStudio MAX及3D-DOCTOR軟件對掃描獲得的橫斷面進行圖像分割并重建牙體外形和根管形態(tài)的,制作牙齒橫斷面掃描圖、根管系統(tǒng)及牙體外形的動畫。以Microsoft Visual Studio2005為系統(tǒng)開發(fā)平臺,C#作為系統(tǒng)開發(fā)語言,采用瀏覽器/服務器模式(B/S方式),通過SQL Server2008數(shù)據(jù)庫管理軟件構建中國人數(shù)字恒牙數(shù)據(jù)庫。 結果：收集到所有牙位共計3309顆中國人離體牙,按照牙位+4位數(shù)字方式將其逐一編號,完成Micro-CT掃描后儲存在樣本柜中。在VGStudio MAX及3D-DOCTOR兩款軟件輔助下,基于離體牙掃描橫斷面原始數(shù)據(jù),共完成3309個牙齒樣本的外形及根管系統(tǒng)重建。把離體牙掃描原始圖像按照從牙冠到根尖的方向制作成3309個橫斷面動畫。將重建的牙體外形和根管系統(tǒng)按照順時針方向旋轉(zhuǎn),分別完成3309個動畫錄制。每個牙齒有三個動畫,實現(xiàn)從外到內(nèi),自上而下,全方位多角度立體觀察牙齒形態(tài)結構。成功構建了中國人恒牙可視化數(shù)據(jù)庫管理系統(tǒng),實現(xiàn)參數(shù)設置、權限設置及用戶信息管理在內(nèi)的系統(tǒng)管理功能；牙齒檔案、文件信息、檔案檢索及文件檢索在內(nèi)的牙齒檔案管理功能。 結論：構建的中國人數(shù)字恒牙數(shù)據(jù)庫信息量豐富,方便我們進行數(shù)據(jù)管理工作并為牙齒解剖教學和臨床根管治療提供較大參考價值。 第二部分含鎂及含鋅硅酸鹽材料成骨和抗菌性能的研究 目的：評價透輝石(DIOP)和鋅黃長石(HT)的理化特性、促進細胞增殖、成骨分化及抗菌性能,探討其在根尖周炎骨缺損修復中的應用前景。 方法：觀察DIOP、HT和β-TCP的表面形貌、元素組成。電感耦合等離子體原子發(fā)射光譜儀測定不同濃度浸提液中離子濃度。采用MTT法和實時熒光定量比較材料對牙周膜細胞(PDLCs)和骨髓間充質(zhì)干細胞(BMSCs)增殖及成骨分化促進作用。細菌計數(shù)及激光共聚焦掃描顯微鏡比較材料對糞腸球菌的抑制效果。 結果：DIOP和HT促進PDLCs細胞增殖,抑制浮游E.faecalis生長的性能優(yōu)于β-TCP,誘導PDLCs骨分化／成牙骨質(zhì)分化效果與P-TCP相當,誘導BMSCs增殖的能力強于β-TCP。HT對E.faecalis及其生物膜的抗菌效果不及Ca(OH)2。 結論：DIOP和HT能夠促進根尖周成骨,HT對糞腸球菌具有一定殺滅作用。兩種硅酸鹽材料對根尖周炎組織的修復再生有潛在應用價值。
[Abstract]:The purpose of the first part is to construct the Chinese permanent teeth database and realize the functions of data management and query. Methods: the isolated teeth of Chinese in different positions were collected and scanned by Micro-CT with a resolution of 30-37 渭 m. VGStudio MAX and 3D-DOCTOR software were used to segment the images and reconstruct the tooth shape and root canal shape. The tooth cross-sectional images root canal system and tooth shape animation were made. The Chinese digital permanent tooth database is constructed by using the browser / server mode (B / S mode) and the SQL Server2008 database management software using Microsoft Visual Studio2005 as the system development platform and C # as the system development language. Results: a total of 3309 Chinese isolated teeth were collected and numbered one by one according to the four digits of tooth position. Micro-CT scanning was completed and stored in the sample cabinet. With the aid of VGStudio MAX and 3D-DOCTOR, a total of 3309 tooth samples were reconstructed based on the original data of tooth scanning in vitro. The original images of isolated teeth were made into 3309 cross-sectional animations according to the direction from crown to root tip. The reconstructed tooth shape and root canal system were rotated clockwise and 3309 animations were recorded. Each tooth has three animations, from outside to inside, top-down, omnidirectional and multi-angle stereoscopic observation of tooth morphology. The visual database management system of Chinese permanent teeth is successfully constructed, which realizes the system management functions, such as parameter setting, permission setting and user information management, tooth files, file information, etc. File retrieval and file retrieval including dental file management function. Conclusion: the Chinese digital permanent teeth database is rich in information, which is convenient for us to carry out data management and provide a great reference value for dental anatomy teaching and clinical root canal therapy. The second part of the study on the osteogenic and antibacterial properties of magnesium and zinc silicate materials objective: to evaluate the physical and chemical properties of diopside (DIOP) and zinc feldspar (HT), and to promote cell proliferation, osteogenic differentiation and antibacterial properties. To explore its application in the repair of bone defect in periapical periodontitis. Methods: the surface morphology and elemental composition of DIOP,HT and 尾-TCP were observed. Inductively coupled plasma atomic emission spectrometer (ICP-AES) was used to determine the ion concentration in the extraction solution with different concentrations. The proliferation and osteogenic differentiation of periodontal ligament cells (PDLCs) and bone marrow mesenchymal stem cells (BMSCs) were studied by MTT and real-time fluorescence quantitative analysis. Bacterial count and laser confocal scanning microscope (LSCM) were used to compare the inhibitory effect of the material on Enterococcus faecalis. Results the proliferation of PDLCs cells was promoted by HT and HT, and the inhibition of E.faecalis growth was superior to that of 尾 -TCP.Osteogenic / cementogenic differentiation of PDLCs was similar to that of P-TCP, and the ability of inducing BMSCs proliferation was stronger than that of 尾 -TCP.HT against E.faecalis and its biofilm. The antibacterial effect of 尾 -TCP.HT on E.faecalis and its biofilm was not as good as that of Ca (OH) 2. ConclusionDIOP and HT can promote periapical osteogenesis to kill Enterococcus faecalis. The two silicate materials have potential application value in the repair and regeneration of periapical tissue.
【學位授予單位】：武漢大學
【學位級別】：博士
【學位授予年份】：2014
【分類號】：R781.05;TP311.13

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