【摘要】：目的:探討轉(zhuǎn)錄因子Foxo3a在釉質(zhì)發(fā)育過程中調(diào)控基質(zhì)金屬蛋白酶-20(MMP-20)基因表達(dá)的分子機制。方法:分別利用免疫組織化學(xué)技術(shù)、免疫熒光、RT-PCR法、雙熒光素酶報告基因檢測系統(tǒng)檢測轉(zhuǎn)錄因子Foxo3a與MMP-20啟動子區(qū)域的轉(zhuǎn)錄調(diào)控水平,分析Foxo3a調(diào)控MMP-20的轉(zhuǎn)錄活性的機制。結(jié)果:免疫組織化學(xué)染色發(fā)現(xiàn)Foxo3a在成釉細(xì)胞核中呈陽性表達(dá),并且隨著釉質(zhì)的分泌表達(dá)逐漸增強,晚期變?nèi)?RT-PCR證實Foxo3a促進(jìn)MMP-20基因表達(dá)(P0.05);雙熒光素酶檢測顯示,Foxo3a促進(jìn)MMP-20表達(dá)(P0.05);特定序列定點突變后的Foxo3a對MMP-20基因的轉(zhuǎn)錄活性無顯著的影響(P0.05)。結(jié)論:轉(zhuǎn)錄因子Foxo3a可能通過與特定序列結(jié)合而調(diào)控MMP-20的表達(dá),從而在釉質(zhì)分泌過程中發(fā)揮重要作用。
[Abstract]:Aim: to investigate the molecular mechanism of transcription factor Foxo3a regulating matrix metalloproteinase-20 (MMP-20) gene expression during enamel development. Methods: immunohistochemistry, immunofluorescence RT-PCR and double luciferase reporter gene detection system were used to detect the transcriptional regulation level of Foxo3a and MMP-20 promoter regions, and to analyze the mechanism of Foxo3a regulating the transcriptional activity of MMP-20. Results: immunohistochemical staining showed that Foxo3a was positively expressed in the ameloblast nucleus and increased with the secretory expression of enamel. RT-PCR confirmed that Foxo3a promoted the expression of MMP-20 gene (P0.05); double luciferase assay showed that Foxo3a promoted MMP-20 expression (P0.05); Foxo3a with specific sequence site-directed mutation had no significant effect on the transcriptional activity of MMP-20 gene (P0.05). Conclusion: transcription factor Foxo3a may play an important role in the process of enamel secretion by regulating the expression of MMP-20 by binding to specific sequences.
【作者單位】： 濰坊醫(yī)學(xué)院口腔醫(yī)學(xué)研究所;濱州醫(yī)學(xué)院附屬醫(yī)院口腔科;
【基金】：國家自然科學(xué)基金(30973327) 山東省自然科學(xué)基金(ZR2010HM076)
【分類號】：R780.2

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