【摘要】：目的：研究EgAgB (Echincoccocus granulosus Antigen B,細(xì)粒棘球蚴B抗原)對STZ (streptozotocin,鏈脲佐菌素)誘導(dǎo)的T1DM (Type1diabetes mellitus,1型糖尿病)模型小鼠牙齦組織中IL-2(白細(xì)胞介素-2)以及IL-4(白細(xì)胞介素-4)基因表達(dá)產(chǎn)生的影響,為今后進(jìn)一步研究EgAgB在T1DM免疫應(yīng)答機(jī)制中所起的作用,以及運(yùn)用免疫療法治療T1DM中的牙齦炎癥提供實驗依據(jù)。方法：20只雌性小鼠按50mg/kg/d的劑量連續(xù)5天腹腔注射STZ制備小鼠T1DM模型,血糖值超過16.7mmol/L且次日復(fù)測仍為此水平,提示小鼠T1DM模型造模成功(20只小鼠兩次血糖監(jiān)測均超過16.7mmol/L),按每組10只分為2組,處理組給予濃度為0.54mg/L的EgAgB100μg/10g連續(xù)5天腹腔注射,對照組給予同等劑量生理鹽水腹腔注射,40周末采用頸椎脫臼法處死小鼠并取其牙齦,用Trizol一步法提取牙齦組織總RNA,逆轉(zhuǎn)錄(RT)反應(yīng)制備cDNA,行FQ-PCR (Fluoresconce quantitative polymerase chain reaction,熒光定量聚合酶鏈反應(yīng)),檢測牙齦中IL-2mRNA、IL-4mRNA基因在處理組和對照組間表達(dá)的變化。結(jié)果：采用SPSS17.0統(tǒng)計軟件包進(jìn)行統(tǒng)計分析結(jié)果提示：IL-2mRNA在EgAgB處理組表達(dá)量顯著低于對照組中的表達(dá)量(P0.05);IL-4mRNA在EgAgB處理組表達(dá)量顯著高于對照組中的表達(dá)量(P0.05)結(jié)論：EgAgB可使T1DM模型小鼠牙齦中IL-2mRNA表達(dá)降低,IL-4mRNA表達(dá)升高, EgAgB可使牙齦Th1/Th2細(xì)胞產(chǎn)生的細(xì)胞因子比值改變,傾向于病理免疫相關(guān)的Th2方向,可影響T1DM牙齦中的免疫應(yīng)答機(jī)制。
[Abstract]:Objective: to study the effect of EgAgB (Echincoccocus granulosus Antigen B, hydatid B antigen on the expression of IL-2 (interleukin-2) and IL-4 (IL-4) gene in gingival tissues of T1DM (Type1diabetes mellitus,1 diabetic) mice induced by STZ (streptozotocin, streptozotocin (STZ). To further study the role of EgAgB in the mechanism of T1DM immune response and to provide experimental evidence for the treatment of gingival inflammation in T1DM by immunotherapy. Methods Twenty female mice were injected intraperitoneally with STZ for 5 days according to the dose of 50mg/kg/d to make T1DM model. The blood glucose level exceeded 16.7mmol/L and was measured again the next day. The results indicated that the T1DM model of mice was successfully established (20 mice were monitored more than 16.7mmol/L twice), 10 mice in each group were divided into 2 groups, and the treatment group was given EgAgB100 渭 g / 10 g of 0.54mg/L concentration intraperitoneally for 5 days. The mice in the control group were killed by cervical dislocations at the end of 40 weeks after intraperitoneal injection of the same dose of normal saline and their gums were taken. The total RNA, reverse transcription (RT) reaction (RNA,) was extracted from gingival tissue by one step Trizol method. CDNA, was prepared by FQ-PCR (Fluoresconce quantitative polymerase chain reaction, fluorescence quantitative polymerase chain reaction (FQ-PCR), and the expression of IL-2mRNA,IL-4mRNA gene in gingiva was detected between the treated group and the control group. Results: the results of statistical analysis using SPSS17.0 statistical software showed that the expression of the weight IL-2 mRNA in the EgAgB treatment group was significantly lower than that in the control group (P0.05). (P0.05) the expression of IL-4 mRNA in the EgAgB treatment group was significantly higher than that in the control group (P0.05). Conclusion: the expression level of the EgAgB treated group is significantly higher than that of the control group (P0.05). The expression of IL-2mRNA in gingiva of T1DM model mice was decreased and the expression of IL-4 mRNA was increased, and the ratio of cytokines produced by Th1/Th2 cells was changed by EgAgB. The direction of Th2 associated with pathological immunity may influence the mechanism of immune response in T1DM gingiva.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R587.2;R781.4

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