【摘要】：目的:研究把牙乳頭干細(xì)胞裝載于富含血小板血漿支架上,植入制備好的人牙根管內(nèi),在SD大鼠皮下組織研究牙髓牙本質(zhì)再生情況,為牙組織再生提供理論依據(jù)以及為該方法用于臨床治療牙髓病提供研究基礎(chǔ)。方法:1、人牙乳頭干細(xì)胞的分離培養(yǎng):取因正畸需拔除的年輕恒牙根尖區(qū)牙乳頭組織,采用組織塊法的方法對(duì)人牙乳頭干細(xì)胞進(jìn)行分離培養(yǎng)及鑒定。2、牙根管的制備:將因正畸拔除的健康無齲的單根管前磨牙無菌條件下去除牙冠和根尖部,根管預(yù)備至200#,再脫抗原處理備用。3、富含血小板血漿支架(PRP)的制備:采取SD大鼠的靜脈全血離心出血漿和血小板,混合制取PRP。4、將人牙乳頭干細(xì)胞裝載在PRP支架植入牙根管內(nèi),埋入SD大鼠皮下培養(yǎng),分為3個(gè)實(shí)驗(yàn)組(SCAP+培養(yǎng)基組,PRP組,SCAP+PRP組),空白牙根管作為對(duì)照組。8周后處死大鼠取出植入物,切片觀察根管內(nèi)牙髓牙本質(zhì)再生情況。結(jié)果:1.對(duì)照組:纖維組織長(zhǎng)入牙根管中,未見到牙髓牙本質(zhì)復(fù)合物的生成。2.SCAP+培養(yǎng)基組:在牙根管內(nèi)可見細(xì)胞植入,但沒有明顯的血管生成,可見大量藍(lán)色的膠原纖維,說明有大量的細(xì)胞外基質(zhì)形成。3.PRP組:在牙根管內(nèi)未見明顯血管生成,有細(xì)胞植入,但未見任何類似牙髓組織的結(jié)構(gòu)形成,可見淺紅色的基質(zhì)。4.SCAP+PRP組:在牙根管內(nèi)有新生組織形成,有類牙髓牙本質(zhì)復(fù)合物生成,細(xì)胞間質(zhì)中有類血管生成,細(xì)胞外基質(zhì)大量分泌,生成較多的膠原纖維。結(jié)論:對(duì)照組,PRP組,SCAP+培養(yǎng)基組都未見明顯的血管形成,未見類牙髓牙本質(zhì)復(fù)合物生成。SCAP+PRP移植SD大鼠皮下,可見大量的新生血管及細(xì)胞外基質(zhì)形成,基質(zhì)不斷沉積,有類牙髓牙本質(zhì)復(fù)合物生成。
[Abstract]:Objective: to study the effect of dental papilla stem cells loaded on platelet-rich plasma scaffold and implanted into human root canal to study pulp dentin regeneration in subcutaneous tissue of SD rats. To provide theoretical basis for dental tissue regeneration and to provide a basis for clinical treatment of dental pulp disease. Methods: 1. Isolation and culture of human dental papilla stem cells: the dental papilla tissue of the apical region of the young permanent teeth needed to be removed due to orthodontic treatment, The human dental papillary stem cells were isolated and identified by tissue mass method. The root canal was prepared by removing the crown and apical region of the healthy and caries free premolars after orthodontic extraction. Root canal preparation to 200 #.3. preparation of platelet-rich plasma stents (PRP): the plasma and platelets were centrifuged from the venous whole blood of SD rats, and PRP.4, was mixed to make human dental papillary stem cells loaded into PRP scaffolds and implanted into root canals. SD rats were subcutaneously cultured and were divided into three experimental groups (SCAP medium group and SCAP group). The blank root canal was used as the control group for 8 weeks. The rats were killed to take out the implants, and the pulp dentin regeneration in the root canal was observed by sections. The result is 1: 1. Control group: the fibrous tissue grew into the root canal, and there was no formation of dental pulp dentin complex. 2. In the SCAP medium group, the cells were implanted in the root canal, but there was no obvious angiogenesis, and a large number of blue collagen fibers were observed. The results showed that there was a large number of extracellular matrix formation. 3. PRP group: there was no significant angiogenesis and cell implantation in the root canal, but there was no formation of any structure similar to dental pulp tissue. In the PRP group, there were new tissue formation, dentin complex formation, angiogenesis, extracellular matrix secretion and more collagen fibers in the root canal. Conclusion: no significant angiogenesis was found in the control group and no significant angiogenesis was found in the control group. However, there was no subcutaneous formation of dental pulp dentin complex. A large number of neovascularization and extracellular matrix formation were observed in SD rats, and the matrix was continuously deposited. Dental pulp-like dentin complex was formed.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R781

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本文編號(hào)：2200057