【摘要】：背景:隨著牙種植體機(jī)制基礎(chǔ)研究的發(fā)展和材料學(xué)的進(jìn)步,種植體支持的義齒得到越來(lái)越多的臨床應(yīng)用。經(jīng)過(guò)20多年的發(fā)展,骨結(jié)合種植義齒目前已成為臨床治療中不可缺少的一部分,成為牙列缺損和牙列缺失的首要修復(fù)方式[1]。種植體與天然牙相比周圍缺乏牙周膜本體感受器,感知覺(jué)閾值比天然牙高[2],且生理動(dòng)度有限,當(dāng)所受外力較大時(shí),會(huì)造成周圍骨破壞,最終導(dǎo)致種植體失敗,降低種植的長(zhǎng)期成功率。因此研究種植體周圍神經(jīng)再生具有重要意義。種植體的表面特征研究一直是人們關(guān)注的焦點(diǎn)。目前,為了提高材料的生物性能,在金屬植入材料的表面引入生物活性涂層,是生物醫(yī)學(xué)研究領(lǐng)域中的研究熱點(diǎn)[3]。鈦(合金)羥基磷灰石復(fù)合材料不僅具有小密度、低毒性、優(yōu)良的生物相容性和較強(qiáng)的抗腐蝕性等鈦(合金)的特征,還具備羥基磷灰石的高生物學(xué)相容性、骨傳導(dǎo)性和生物活性等特征,以滿足骨及牙齒等的修復(fù)與替換的生物學(xué)性能和力學(xué)需求[4-6],而且羥基磷灰石種植體表面可以促進(jìn)神經(jīng)細(xì)胞的生物學(xué)行為和神經(jīng)傳導(dǎo)性能[7-8]。已被廣泛應(yīng)用于牙種植領(lǐng)域中,已成為一種重要的臨床生物材料產(chǎn)品。實(shí)驗(yàn)?zāi)康呐c方法:第一部分:羥基磷灰石涂層的制備及不同沉積時(shí)間對(duì)“鈦骨”表面電化學(xué)沉積羥基磷灰石涂層的理化性質(zhì)的影響。在保證其它實(shí)驗(yàn)參數(shù)不變的情況下,運(yùn)用計(jì)時(shí)電位—電化學(xué)沉積法在“鈦骨”表面構(gòu)建不同沉積時(shí)間的羥基磷灰石涂層,從0-80min共分為5組。研究電解液時(shí)間對(duì)電沉積涂層的形貌、化學(xué)成分及親水性的影響,從而確定最優(yōu)的電沉積參數(shù)。第二部分:改良“鈦骨”種植體對(duì)雪旺細(xì)胞黏附,增殖的影響通過(guò)細(xì)胞黏附實(shí)驗(yàn)選出利于細(xì)胞黏附,增殖的涂層表面,為改良“鈦骨”種植體表面設(shè)計(jì)提供實(shí)驗(yàn)依據(jù)。實(shí)驗(yàn)結(jié)果:1、不同沉積時(shí)間沉積涂層為羥基磷灰石,涂層厚度隨沉積時(shí)間的增加而增厚,沉積時(shí)間為40min-80min的涂層厚度為0.8-3.5μm。沉積時(shí)間為20min組,材料表面可見晶體呈顆粒狀雜亂分布,排列較稀疏。40/60/80min組可見“鈦骨”表面微納米級(jí)大小的晶體排列有序,沉積時(shí)間越長(zhǎng),晶粒排列越密集,形成交聯(lián)多孔的網(wǎng)狀表面,晶粒大小約為80-100nm,均勻分布;2、對(duì)照組“鈦骨”表面是疏水表面,電沉積羥基磷灰石涂層后變?yōu)橛H水表面。沉積時(shí)間越長(zhǎng),涂層越厚,接觸角越小。其中40/60/80min組表現(xiàn)出明顯的親水性;3、納米羥基磷灰石改良“鈦骨”表面,雪旺細(xì)胞黏附形態(tài)較好,細(xì)胞有粗大的偽足附著在多孔網(wǎng)狀表面,與涂層相融合且細(xì)胞形態(tài)及直徑大小均優(yōu)于相應(yīng)純鈦涂層組;4、40/60/80min處理的羥基磷灰石“鈦骨”表面均可促進(jìn)雪旺細(xì)胞的粘附和增殖。實(shí)驗(yàn)結(jié)論:1、通過(guò)電化學(xué)沉積法可以在“鈦骨”表面沉積羥基磷灰石,40/60/80min時(shí)晶體排列密集,分布均一,呈相互交聯(lián)多孔網(wǎng)狀結(jié)構(gòu)表面,晶體大小約80-100nm,涂層厚度隨沉積時(shí)間的增加而增厚;2、納米羥基磷灰石改良“鈦骨”表面為親水表面,40/60/80min組親水性顯著;3、40/60/80min處理的羥基磷灰石“鈦骨”表面均可促進(jìn)雪旺細(xì)胞的粘附和增殖;
[Abstract]:Background: with the development of the basic research of dental implant mechanism and the progress of material science, implant supported denture has been more and more clinical. After more than 20 years of development, bone combined implant has become an indispensable part of clinical treatment, and become the primary repair mode of dentition loss and dentition loss [1]. implant. There is a lack of periodontal ligament proprioceptor around natural teeth. The perception threshold is [2] higher than that of natural teeth, and its physiological mobility is limited. When the external force is large, it will cause destruction of the surrounding bone and eventually lead to the failure of the implant and reduce the long-term success rate of the implant. Therefore, it is of great significance to study the regeneration of the nerve around the implant. At present, in order to improve the biological properties of materials, the introduction of bioactive coatings on the surface of metal implanted materials is a hot spot in the field of biomedical research, [3]. titanium (alloy) hydroxyapatite composite not only has small density, low toxicity, excellent biocompatibility and strong anti-corruption. The characteristics of corrosion, such as titanium (alloy), are also characterized by high biological compatibility, bone conductivity and biological activity of hydroxyapatite, to meet the biological and mechanical requirements of the repair and replacement of bone and teeth [4-6], and the hydroxyapatite implant surface can promote the biological behavior and nerve conduction performance of the nerve cells [7- 8]. has been widely used in dental implant field and has become an important clinical biomaterial product. Experimental purposes and methods: the first part: the preparation of hydroxyapatite coating and the effect of different deposition time on the physical and chemical properties of the electrodeposited hydroxyapatite coating on the "titanium bone" surface. The hydroxyapatite coating on the surface of "titanium bone" was constructed on the surface of "titanium bone" by Chrono potential electrodeposition, and divided into 5 groups from 0-80min. The effect of electrolyte time on the morphology, chemical composition and hydrophilicity of the electrodeposited coating was studied to determine the optimal electrodeposition parameters. The second part: improving the cultivation of "titanium bone" The effect of body adhesion to Schwann cells and proliferation was selected by cell adhesion experiment to select the surface of cell adhesion and proliferation, which provided experimental basis for improving the surface design of "titanium bone" implant. The experimental results were as follows: 1, the coating was deposited as hydroxyapatite at different deposition time, and the thickness of the coating thickened with the increase of deposition time, and the deposition time was 40. The coating thickness of min-80min is 0.8-3.5 M. deposition time of 20min group, and the crystal shows granular and random distribution on the surface of the material. The thin and nanoscale crystals of the "titanium bone" surface are arranged in a more orderly arrangement than the sparse.40/60/80min group. The longer the deposition time is, the more dense the grain is arranged, and the cross-linked porous network surface is formed. The size of the grain is about 8. 2, 2, the surface of the titanium bone in the control group is a hydrophobic surface, and the electrodeposited hydroxyapatite coating becomes hydrophilic surface. The longer the deposition time is, the thicker the coating is, the smaller the contact angle is, and the 40/60/80min group shows obvious hydrophilicity, and the nano hydroxyapatite improves the "titanium bone" surface and the cell adhesion of Schwann cells is better. The thick pseudo foot attached to the porous network surface, fused with the coating, and the morphology and diameter of the cells were superior to that of the corresponding pure titanium coating group; the surface of the hydroxyapatite "titanium bone" treated by 4,40/60/80min could promote the adhesion and proliferation of Schwann cells. Experimental conclusion: 1, electrochemistry deposition can be used to deposit hydroxyl on the surface of "titanium bone" by electrochemical deposition. The basic apatite, 40/60/80min, is densely arranged and distributes uniformly. The surface of the porous network structure is cross-linked with each other. The size of the crystal is about 80-100nm. The thickness of the coating thickens with the increase of the deposition time. 2, the nano hydroxyapatite improves the surface of the titanium bone as the hydrophilic surface, and the hydrophilic property of the 40/60/ 80min group is significant; the hydroxyapatite treated by 3,40/60/80min is the hydroxyapatite treated. The surface of titanium bone can promote the adhesion and proliferation of Schwann cells.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R783.6

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