本文選題：牙髓細胞 + 脂多糖　； 參考：《青島大學》2014年碩士論文

【摘要】：目的： 本實驗通過不同濃度的牙齦卟啉單胞菌(Porphyromonas gingivalis, Pg)脂多糖(lipopolysaccharide, LPS)刺激體外培養(yǎng)的牙髓細胞,檢測不同時間下牙髓細胞中各種礦化相關(guān)因子(DSPP、ALP、BSP)的表達情況,研究LPS對牙髓細胞礦化能力的影響。 方法： 采用組織塊法獲得大鼠牙髓細胞,并用免疫組化方法對培養(yǎng)細胞進行鑒定,以含0.1ng/ml、1ng/ml、10ng/ml、100ng/ml和10000ng/ml牙齦卟啉單胞菌(Porphyromonas gingivalis, Pg) LPS作用牙髓細胞1、3、5d,用熒光定量反轉(zhuǎn)錄聚合酶鏈反應(yīng)(實時定量PCR)檢測DSPP. ALP. BSP mRNA表達的變化,采用spss17.0軟件包對實驗數(shù)據(jù)分別進行統(tǒng)計學分析。 結(jié)果： 鏡下貼壁后的細胞形態(tài)多樣,多呈成纖維樣細胞形態(tài),還有部分多角形細胞,胞漿突起。實時定量PCR結(jié)果顯示：與對照組相比,1ng/ml、10ng/ml LPS組,大鼠牙髓細胞DSPP. ALP. BSP的mRNA表達增高,100ng/ml、10000ng/ml LPS組,DSPP、ALP、BSP的mRNA表達均降低；在第1、3、5d時1ng/ml、10ng/ml、100ng/ml和10000ng/ml LPS組：mRNA表達逐漸減少。0.1ng/ml LPS對]mRNA的表達變化無明顯差異。3種因子呈現(xiàn)相似的表達變化趨勢。 結(jié)論： 低劑量PgLPS有促進牙髓細胞ALP、BSP、DSPP的表達,高劑量時抑制ALP、BSP、DSPP的表達；隨著培養(yǎng)時間的延長,促進作用逐漸減弱,抑制作用逐漸增強。
[Abstract]:Objective: to investigate the expression of various mineralization-related factors (DSPP- ALPP-BSP) in dental pulp cells stimulated by different concentrations of Porphyromonas gingivalis (PG) lipopolysaccharide (LPS) in vitro. To study the effect of LPS on the mineralization ability of dental pulp cells. Methods: rat dental pulp cells were obtained by tissue mass method and identified by immunohistochemistry. DSPP was detected by fluorescence quantitative reverse transcriptase polymerase chain reaction (real-time PCR) for 5 days in dental pulp cells treated with 10 ng / ml 10 ng / ml 10000ng/ml and Porphyromonas gingivalis (PG) for 5 days. ALP. The changes of BSP mRNA expression were statistically analyzed by spss17.0 software package. Results: the morphologies of the cells attached to the wall under microscope were various, mostly fibroblast-like cells, and some polygonal cells with cytoplasmic processes. The results of real-time quantitative PCR showed that compared with the control group, DSPPin rat dental pulp cells were treated with 1 ng / ml 10 ng / ml LPS. ALP. The expression of BSP mRNA increased and the expression of BSP mRNA decreased in 100 ng / ml LPS group, but at the first day of 3 days, the mRNA expression of 1 ng / ml 10ng / ml 10000ng/ml decreased gradually. 0.1 ng / ml LPS showed no significant difference in the change of mRNA expression. 3. 3 factors showed a similar trend of expression of BSP mRNA in the LPS-treated group and the LPS group showed no significant difference in the change of the expression of BSP mRNA and the expression of BSP mRNA in the LPS group (P < 0.05), but there was no significant difference in the change of the mRNA expression between the LPS group and the LPS-treated group (P < 0.01 ng / ml). Conclusion: low dose of PgLPS can promote the expression of DSPP in dental pulp cells, inhibit the expression of DSPP in ALP- BSPP at high dose, and weaken the promoting effect and increase the inhibitory effect with the prolongation of culture time.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R781

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