本文選題：牙周炎 + 動(dòng)物模型�。� 參考：《上�？谇会t(yī)學(xué)》2014年06期

【摘要】：目的 :通過口腔涂抹牙周致病菌的方法建立小鼠牙周炎模型,比較低齡小鼠與老齡小鼠牙周炎發(fā)病免疫狀態(tài)差異,分析Th17/Treg細(xì)胞相關(guān)因子在不同年齡牙周炎小鼠中的表達(dá)情況。方法:采用實(shí)體顯微鏡評(píng)價(jià)牙周骨吸收的程度,H-E染色法觀察牙周組織病變的炎細(xì)胞浸潤情況,TRAP染色法觀察牙周骨組織表面破骨細(xì)胞浸潤情況,實(shí)時(shí)定量PCR檢測牙齦組織中Th17/Treg細(xì)胞相關(guān)因子TGF-β1、IL-10、IL-17A、RANKL m RNA的表達(dá)水平。采用SPSS17.0軟件包對數(shù)據(jù)進(jìn)行統(tǒng)計(jì)學(xué)分析。結(jié)果:實(shí)驗(yàn)4周后,牙周炎組與正常對照組相比,釉-牙骨質(zhì)界至牙槽嵴頂?shù)木嚯x顯著增加(P0.01),老齡組小鼠釉-牙骨質(zhì)界至牙槽嵴頂?shù)木嚯x比低齡組小鼠顯著增加(P0.01);牙周組織中大量炎細(xì)胞浸潤,牙周袋加深;牙周組織中TGF-β1、IL-10 m RNA的表達(dá)水平顯著降低(P0.01),IL-17A、RANKL m RNA表達(dá)水平顯著增高(P0.01)。結(jié)論:老齡小鼠牙周炎中炎癥介質(zhì)的表達(dá)存在異常狀態(tài),比低齡小鼠牙周組織病損狀態(tài)加劇,提示牙周炎的炎癥狀態(tài)可能不僅與其分泌的免疫抑制性細(xì)胞因子表達(dá)降低有關(guān),更與其增齡性相關(guān)。
[Abstract]:Objective: to establish the periodontitis model of mice by oral smearing periodontal pathogenic bacteria, compare the difference of immune state of periodontitis between young mice and aged mice, and analyze the expression of Th17 / Treg cytokines in periodontitis mice of different ages. Methods: the degree of periodontal bone resorption was evaluated by solid microscope and the infiltration of inflammatory cells in periodontal tissue was observed by H-E staining and the infiltration of osteoclasts on the surface of periodontal bone tissue was observed by trap staining. The expression of TGF- 尾 1, IL-10, IL-17, RANKL mRNA in gingival tissues was detected by real-time quantitative PCR. SPSS 17.0 software package was used to analyze the data. Results: after 4 weeks of experiment, the periodontitis group was compared with the normal control group. The distance between enamel-cementum boundary and alveolar crest was significantly increased (P0.01), and the distance between enamel-cementum boundary and alveolar crest in aging group was significantly higher than that in younger group (P0.01), a large number of inflammatory cells infiltrated in periodontal tissue and periodontal bag deepened. The expression of IL-10 mRNA in periodontal tissues was significantly decreased (P0.01) and the expression of IL-17A1RANKL mRNA was significantly increased (P0.01). Conclusion: the expression of inflammatory mediators in periodontitis in aged mice is abnormal, which is more serious than that in young mice, suggesting that the inflammatory state of periodontitis may not only be related to the decreased expression of immunosuppressive cytokines secreted by periodontitis. It was also related to its aging.
【作者單位】： 中國醫(yī)科大學(xué)附屬盛京醫(yī)院 口腔科;
【基金】：高等學(xué)校博士學(xué)科點(diǎn)專項(xiàng)科研基金(20112104110013) 遼寧省科學(xué)技術(shù)計(jì)劃項(xiàng)目(2012225015) 沈陽市科技計(jì)劃項(xiàng)目(F13-220-9-28)~~
【分類號(hào)】：R781.42

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