本文選題：牙周膜成纖維細(xì)胞 + 三維鈦網(wǎng)支架　； 參考：《重慶醫(yī)科大學(xué)》2014年碩士論文

【摘要】：根據(jù)牙周膜成纖維細(xì)胞能向成牙骨質(zhì)、成骨方向分化的多向分化潛能，，通過(guò)將犬牙周膜成纖維細(xì)胞混合細(xì)胞外基質(zhì)，接種至三維鈦網(wǎng)支架材料內(nèi)部，植入裸鼠皮下，觀察其形成類牙周結(jié)構(gòu)的可能性。 利用膠原酶、胰蛋白酶消化組織塊法原代培養(yǎng)獲取犬牙周膜成纖維細(xì)胞，利用倒置顯微鏡、HE染色觀察其細(xì)胞外形及生長(zhǎng)方式，透射電鏡觀察細(xì)胞超微結(jié)構(gòu)，通過(guò)免疫細(xì)胞化學(xué)染色對(duì)犬牙周膜進(jìn)行來(lái)源鑒定，通過(guò)礦化結(jié)節(jié)實(shí)驗(yàn)證明其能向硬組織分化；用ECM gel混合犬牙周膜成纖維細(xì)胞到三維鈦網(wǎng)支架材料內(nèi)部，掃描電鏡發(fā)現(xiàn)種子細(xì)胞在支架材料表面附著增殖程度較傳統(tǒng)反復(fù)浸透法優(yōu)良；硬組織切片發(fā)現(xiàn)有硬組織染色，且部分樣本產(chǎn)生類似膠原纖維。
[Abstract]:According to the multidirectional differentiation potential of periodontal ligament fibroblasts into cementoblasts and osteoblasts, the canine perodontic ligament fibroblasts were mixed with extracellular matrix and inoculated into the three-dimensional titanium mesh scaffold and implanted subcutaneously into nude mice. The possibility of periodontal formation was observed. The canine periodontic membrane fibroblasts were obtained by using collagenase and trypsin digestion tissue block method. The morphology and growth pattern of the cells were observed by HE staining with inverted microscope, and the ultrastructure of the cells was observed by transmission electron microscope (TEM). The origin of canine periodontic membrane was identified by immunocytochemical staining, and it was proved by mineralized nodules that it could differentiate into hard tissue. Scanning electron microscopy showed that seed cells had better adhesion and proliferation on the surface of scaffolds than the traditional repeated immersion method. Hard tissue staining was found in hard tissue sections and some samples produced collagen-like fibers.
【學(xué)位授予單位】：重慶醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R783.1

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 朱衛(wèi)東;侯建霞;劉凱寧;孟煥新;唐曉琳;;自體牙周膜細(xì)胞和釉基質(zhì)蛋白修復(fù)猴下后牙Ⅲ度根分叉病變[J];北京大學(xué)學(xué)報(bào)(醫(yī)學(xué)版);2009年01期

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