發(fā)布時(shí)間：2018-06-20 06:19

  本文選題：低氧預(yù)處理 + 腺苷酸單磷酸激活蛋白激酶(AMPK)��； 參考：《牙體牙髓牙周病學(xué)雜志》2014年07期

【摘要】：目的:觀察低氧預(yù)處理以及腺苷酸單磷酸激活蛋白激酶(AMPK)過(guò)表達(dá)對(duì)過(guò)氧化氫(H2O2)抑制牙髓細(xì)胞增殖的影響。方法:牙髓細(xì)胞分別在常氧(950 mL/L O2)和低氧(10 mL/L O2)環(huán)境下培養(yǎng)12 h后,用qRT-PCR檢測(cè)細(xì)胞中AMPK mRNA的表達(dá)水平;分別取常氧和低氧培養(yǎng)12 h的牙髓細(xì)胞與0.1 mmol/L H2O2共培養(yǎng)4 h后,常規(guī)條件下繼續(xù)培養(yǎng)4 d,用MTT法檢測(cè)細(xì)胞的增殖能力;分別用AMPK過(guò)表達(dá)質(zhì)粒和特異性小RNA干擾(siRNA)片段轉(zhuǎn)染牙髓細(xì)胞,與0.1 mmol/L H2O2共培養(yǎng)4 h,然后常規(guī)條件下繼續(xù)培養(yǎng)4 d,用MTT法檢測(cè)細(xì)胞的增殖能力。結(jié)果:低氧預(yù)處理能顯著上調(diào)牙髓細(xì)胞中AMPK mRNA的表達(dá)水平,并能減輕H2O2對(duì)細(xì)胞增殖的抑制作用(P0.05);AMPK過(guò)表達(dá)細(xì)胞經(jīng)過(guò)H2O2處理后,增殖能力明顯高于正常細(xì)胞+H2O2組和siRNA+H2O2組(P0.05),而siRNA干擾的細(xì)胞經(jīng)H2O2處理后其增殖能力則較其他各組降低(P0.05)。結(jié)論:低氧預(yù)處理和AMPK基因的上調(diào)可使牙髓細(xì)胞獲得對(duì)H2O2氧化的抗性,從而減輕H2O2對(duì)細(xì)胞增殖的抑制作用。
[Abstract]:Aim: to investigate the effects of hypoxia preconditioning and overexpression of adenylate monophosphate-activated protein kinase (AMPK) on the proliferation of dental pulp cells inhibited by H _ 2O _ 2 H _ 2O _ 2. Methods: dental pulp cells were cultured in normoxic (950 mL / L) and hypoxia (10 mL / L) for 12 h. The expression of AMPK mRNA in dental pulp cells was detected by qRT-PCR and co-cultured with 0.1 mmol / L H _ 2O _ 2 for 4 h in normoxic and hypoxic culture for 12 h. The proliferation of dental pulp cells was detected by MTT assay and transfected into dental pulp cells by AMPK overexpression plasmids and specific small RNA interference siRNAs. The cells were co-cultured with 0.1 mmol / L H _ 2O _ 2 for 4 h, then cultured for 4 days under conventional conditions. The proliferation of the cells was measured by MTT assay. Results: hypoxia pretreatment could significantly up-regulate the expression of AMPK mRNA in dental pulp cells and reduce the inhibitory effect of H _ 2O _ 2 on cell proliferation. The proliferative ability of siRNA interference cells was significantly higher than that of normal cells treated with H 2O 2 and siRNA H 2O 2 groups, but the proliferation ability of siRNA interference cells treated with H 2O 2 was lower than that of other groups. Conclusion: hypoxia preconditioning and up-regulation of AMPK gene can make dental pulp cells become resistant to H2O2 oxidation, thus reducing the inhibitory effect of H2O2 on cell proliferation.
【作者單位】： 安康市中心醫(yī)院口腔科;神木縣醫(yī)院口腔科;
【分類號(hào)】：R780.2
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本文編號(hào)：2043285