本文選題：牙周膜干細(xì)胞 + 年齡因素��； 參考：《南方醫(yī)科大學(xué)學(xué)報(bào)》2017年03期

【摘要】：目的評(píng)估年齡因素對(duì)牙周膜干細(xì)胞(PDLSC)增殖、分化能力的影響。方法選取來(lái)源于12位不同的患者因阻生需要拔除的第三磨牙12顆,根據(jù)患者年齡分為A組(年齡18~20歲)、B組(年齡45~50歲)。分離培養(yǎng)獲得PDLSC。MTT檢測(cè)A、B組PDLSC增殖能力;進(jìn)行成骨、成脂誘導(dǎo),并用茜素紅染色及油紅O染色評(píng)價(jià)其分化能力;SA-βG染色分析A、B組細(xì)胞衰老情況;將A、B組PDLSC成骨誘導(dǎo)7 d后進(jìn)行ALP染色并同時(shí)采用Western blotting檢測(cè)OCN、ALP的表達(dá)。結(jié)果不同年齡階段的供體中都能分離出PDLSC,然而其增殖和成骨分化能力隨年齡增加而降低,成脂分化能力和SA-βG表達(dá)隨年齡增加而增加。ALP和OCN成骨誘導(dǎo)7 d后表達(dá)水平均升高,且A組高于B組。結(jié)論隨年齡增長(zhǎng)能在牙周膜中獲取PDLSC,隨年齡增加其增殖與成骨分化能力下降,但成脂分化能力和SA-β表達(dá)則增加。
[Abstract]:Objective to evaluate the effect of age on the proliferation and differentiation of periodontal ligament stem cells (PDLSCs). Methods Twelve third molars from 12 patients were divided into group A (n = 18, 20 years old) and B group (n = 45 ~ 50 years old) according to the age of the patients. PDLSC.MTT assay was used to detect the proliferation of PDLSC, osteogenesis and lipogenesis were performed. The differentiation ability of PDLSC was evaluated by alizarin red staining and oil red O staining. ALP staining was performed 7 days after osteogenesis induction of PDLSC in Agna B group and the expression of OCN-ALP was detected by Western blotting. Results PDLSCs could be isolated from donors at different age stages. However, the proliferation and osteogenic differentiation ability of PDLSCs decreased with age, and the expression of SA- 尾 G and adipogenic differentiation increased with age. ALP and OCN increased after osteogenic induction for 7 days. Group A was higher than group B. Conclusion PDLSCs can be obtained from periodontal ligament with age, but the ability of adipogenic differentiation and SA- 尾 expression increase with age.
【作者單位】： 中國(guó)人民解放軍總醫(yī)院口腔科;中國(guó)科學(xué)院理化技術(shù)研究所;
【基金】：國(guó)家自然科學(xué)基金(31670998,51473175) 北京市科技新星計(jì)劃(Z14144000180000-2014101) 中國(guó)博士后科學(xué)基金特別資助項(xiàng)目(2014T70959)~~
【分類號(hào)】：R781.4

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1 毛釗;影響牙周膜細(xì)胞生物學(xué)功能的相關(guān)因素[J];醫(yī)學(xué)研究生學(xué)報(bào);2003年06期

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