本文選題：滑膜干細胞 + 石墨烯　； 參考：《口腔醫(yī)學(xué)研究》2017年04期

【摘要】：目的:探究水溶性石墨烯轉(zhuǎn)染滑膜間充質(zhì)干細胞的的效果及各組間細胞克隆形成率的差別比較。方法:將傳代培養(yǎng)到第3代的滑膜間充質(zhì)干細胞分成實驗組與對照組,實驗組細胞培養(yǎng)基內(nèi)加入濃度為25mol/mL的水溶性石墨烯在37℃的條件下培養(yǎng)24h,對照組用普通培養(yǎng)基培養(yǎng)37℃24h,405nm激發(fā)波長下的激光共聚膠顯微鏡下觀察石墨烯是否成功轉(zhuǎn)染滑膜干細胞。各組間細胞克隆形成率的差別采用單因素方差分析比較。結(jié)果:實驗組的細胞培養(yǎng)24h后,在激光共聚焦顯微鏡下細胞質(zhì)可見發(fā)光的熒光點,細胞核的光強度較弱。各組克隆形成率均P0.05有顯著差異。結(jié)論:石墨烯容易滲透到細胞質(zhì)但沒有進入細胞核,可以猜測石墨烯在進入活的滑膜間充質(zhì)干細胞后沒有發(fā)生遺傳的中斷。
[Abstract]:Aim: to investigate the effect of water-soluble graphene transfection on synovial mesenchymal stem cells and the difference of cell clone formation rate among the groups. Methods: the third generation of synovial mesenchymal stem cells were divided into experimental group and control group. In the experimental group, 25 mol / mL water-soluble graphene was added to the cell culture medium for 24 hours at 37 鈩，

本文編號：2034902