發(fā)布時間：2018-06-09 07:13

  本文選題：舌癌 + 白花丹素　； 參考：《南昌大學(xué)》2014年碩士論文

【摘要】：目的： 探討白花丹素聯(lián)合術(shù)前誘導(dǎo)化療PF方案對舌癌細(xì)胞Tca8113的化療增敏作用及其相關(guān)機(jī)制。 方法： 1.舌癌Tca-8113細(xì)胞的細(xì)胞培養(yǎng)。 2.依次加入白花丹素、順鉑、5氟尿嘧啶，應(yīng)用MTT法檢測舌癌細(xì)胞抑制率，并計算最佳實(shí)驗(yàn)濃度IC10。 3. MTT法檢測舌癌Tca8113細(xì)胞的細(xì)胞抑制率。 4.流式細(xì)胞術(shù)檢測舌癌Tca-8113細(xì)胞的凋亡率。 5. Western Blot法檢測舌癌Tca-8113細(xì)胞中PI3K、P-AKT蛋白表達(dá)水平的變化。 6. ELISA法檢測舌癌Tca-8113細(xì)胞中PI3K、P-AKT蛋白表達(dá)水平的變化。 結(jié)果： 1. MTT實(shí)驗(yàn)表明，白花丹素、順鉑、5氟尿嘧啶作用于舌癌Tca8113細(xì)胞24小時后，通過軟件計算，其生長抑制濃度IC10依次為1.35μm/L、0.006μm/L、0.005μm/L。 2. MTT法檢測結(jié)果表明，白花丹素聯(lián)合PF方案較PF方案單獨(dú)作用，其舌癌細(xì)胞的抑制率有顯著增高（P0.05）。 3.流式細(xì)胞術(shù)檢測結(jié)果表明，白花丹素聯(lián)合PF方案較PF方案單獨(dú)作用其舌癌細(xì)胞的凋亡率有顯著增高（P0.05）。 4. Western Blot法檢測結(jié)果顯示，白花丹素聯(lián)合PF方案較單獨(dú)運(yùn)用PF方案，其舌癌Tca8113細(xì)胞中PI3K、P-AKT蛋白表達(dá)水平顯著降低（P0.05）。 5. ELISA法檢測結(jié)果顯示，白花丹素聯(lián)合PF方案較單獨(dú)運(yùn)用PF方案，其舌癌細(xì)胞Tca8113中PI3K、P-AKT蛋白表達(dá)水平顯著降低（P0.05）。 結(jié)論： 白花丹素具有化療增敏作用，，增強(qiáng)了術(shù)前誘導(dǎo)化療PF方案對舌癌Tca8113細(xì)胞的殺傷作用，抑制細(xì)胞中PI3K/AKT信號通路可能是其化療增敏的主要機(jī)制。
[Abstract]:Objective: to investigate the chemosensitizing effect of Baihuadan combined with preoperation induced chemotherapy PF regimen on tongue cancer cell Tca8113. Methods: 1. Cell culture of Tca-8113 cells from tongue cancer. 2. The inhibitory rate of tongue cancer cells was detected by MTT assay, and the optimal concentration of IC10.3 was calculated by adding Bahuadanthin and cisplatin 5 fluorouracil in turn. MTT assay was used to detect the cell inhibitory rate of Tca8113 cells. The apoptosis rate of Tca-8113 cells was detected by flow cytometry. Western Blot assay was used to detect the expression of PI3KTP-AKT protein in Tca-8113 cells. Elisa method was used to detect the expression level of PI3KTP-AKT in Tca-8113 cells. Results: 1. MTT assay showed that the inhibitory concentration of IC10 was 1.35 渭 m / L ~ 0.006 渭 m / L ~ (0.005) 渭 m 路L ~ (-1) 路L ~ (-2) after 24 hours treatment of Tca8113 cell line with Baihuadan and cisplatin _ 5 fluorouracil. The IC10 concentration was 1.35 渭 m 路L ~ (-1) 路L ~ (-1) 路L ~ (-1) 路L ~ (-1). MTT assay showed that compared with PF regimen alone, the inhibition rate of tongue cancer cells was significantly higher than that of PF regimen. The results of flow cytometry showed that compared with PF regimen alone, the apoptosis rate of tongue cancer cells was significantly higher than that of PF regimen alone. Western blot analysis showed that compared with PF alone, the expression of PI3KU P-AKT protein in Tca8113 cells decreased significantly. The results of Elisa showed that compared with PF regimen alone, the expression of PI3KU P-AKT protein in Tca8113 cells of tongue decreased significantly. Conclusion: Baihuadantin has the effect of chemosensitization, and the expression of PI3KUP-AKT protein in Tca8113 tongue cancer cells is significantly lower than that of PF regimen alone. The cytotoxicity of PF regimen to Tca8113 cells was enhanced, and the inhibition of PI3K / AKT signaling pathway may be the main mechanism of chemosensitization.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R739.86

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本文編號：1999502