本文選題：人乳牙牙髓干細胞 + 轉(zhuǎn)化生長因子β�。� 參考：《實用醫(yī)學(xué)雜志》2014年12期

【摘要】：目的:探討轉(zhuǎn)化生長因子β3(TGF-β3)誘導(dǎo)人乳牙牙髓干細胞(SHED)向成牙本質(zhì)樣細胞分化的能力。方法:采用酶消化法將人乳牙牙髓分離培養(yǎng),獲得SHED;對體外培養(yǎng)的第3代SHED分別單獨加入25 ng/mL的重組TGF-β3,或與肝素聯(lián)合進行培養(yǎng);通過Q-PCR和Western-blotting方法,分別觀察SHED表達成牙本質(zhì)細胞特異性標志物-牙本質(zhì)涎磷蛋白(DSPP)基因及其基因表達產(chǎn)物-牙本質(zhì)涎蛋白(DSP)的情況;通過堿性磷酸酶(AKP)試劑盒檢測SHED的AKP活性的改變;細胞爬片行免疫化學(xué)染色和茜素紅染色。結(jié)果:SHED在誘導(dǎo)體系中生長狀態(tài)良好。25 ng/mL TGF-β3聯(lián)合10 U/mL肝素作用組的AKP活性明顯增強,與TGF-β3單獨作用組、肝素單獨作用組以及對照組相比差異具有統(tǒng)計學(xué)意義(P0.01);TGF-β3聯(lián)合肝素作用組的茜素紅染色呈強陽性,Q-PCR和Western-blotting結(jié)果均顯示,該組的DSPP表達在mRNA水平和蛋白質(zhì)水平上均明顯升高。結(jié)論:在TGF-β3與肝素聯(lián)合作用的誘導(dǎo)下,可促進SHED分化為成牙本質(zhì)樣細胞。
[Abstract]:Aim: to investigate the ability of transforming growth factor 尾 3 (TGF- 尾 3) to induce the differentiation of human deciduous dental pulp stem cells (SHED) into odontoblast like cells. Methods: the pulp of human deciduous teeth was isolated and cultured by enzyme digestion, and then the third generation SHED was cultured separately with 25 ng/mL recombinant TGF- 尾 3, or in combination with heparin. Q-PCR and Western-blotting methods were used. The expression of dentin sialophosphate protein (DSPP) gene and its gene expression product, dentine sialoprotein (DSPP), were observed by SHED, and the changes of AKP activity of SHED were detected by alkaline phosphatase (ALP) kit. The cells were stained with immunochemical staining and alizarin red staining. Results the AKP activity of the 10 U/mL heparin treated with 10 U/mL heparin was significantly increased in the growth state of 1: SHED. 25 ng/mL TGF- 尾 3 and TGF- 尾 3 alone. There was significant difference between heparin alone group and control group. The results of alizarin red staining and Q-PCR and Western-blotting showed that the expression of DSPP in the group treated with heparin alone was significantly higher than that in the group treated with heparin. The expression of DSPP in the group was significantly higher than that in the group treated with heparin at the level of mRNA and protein. Conclusion: TGF- 尾 3 combined with heparin can promote the differentiation of SHED into odontoid cells.
【作者單位】： 廣東省口腔醫(yī)院 南方醫(yī)科大學(xué)附屬口腔醫(yī)院;廣東省珠海市口腔醫(yī)院;中山大學(xué)孫逸仙紀念醫(yī)院;
【分類號】：R780.2

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本文編號：1977056