本文選題：Bcl-2 + Bax��； 參考：《新疆醫(yī)科大學(xué)》2017年碩士論文

【摘要】：目的:觀察咬合支持喪失大鼠腦組織海馬區(qū)凋亡相關(guān)基因Bcl-2、Bax的表達(dá),并分析其相關(guān)性。方法:選擇四周齡SD大鼠50只,隨機(jī)分為正常對照組、三顆磨牙缺失組、六顆磨牙缺失組、九顆磨牙缺失組與十二顆磨牙缺失組,每組各十只大鼠。咬合支持喪失組拔除相應(yīng)牙數(shù)后常規(guī)飼養(yǎng)20周,然后取大鼠腦部海馬組織。采用HE染色法觀察各組腦組織海馬區(qū)細(xì)胞形態(tài)結(jié)構(gòu)改變,免疫組化染色法觀察各組腦組織海馬區(qū)Bcl-2、Bax陽性細(xì)胞表達(dá)。結(jié)果:正常對照組腦組織細(xì)胞形態(tài)結(jié)構(gòu)正常,基本無損傷性改變。三顆磨牙缺失組細(xì)胞無明顯變化;六顆磨牙缺失組出現(xiàn)細(xì)胞層數(shù)減少,結(jié)構(gòu)排列紊亂;九顆磨牙缺失組海馬組織神經(jīng)細(xì)胞數(shù)目明顯減少,細(xì)胞排列疏松;十二顆磨牙缺失組海馬組織細(xì)胞內(nèi)出現(xiàn)神經(jīng)細(xì)胞數(shù)量銳減。咬合支持喪失組各組腦組織海馬區(qū)Bcl-2陽性細(xì)胞數(shù)均較正常對照組顯著減少,咬合支持喪失組各組腦組織海馬區(qū)Bax陽性細(xì)胞數(shù)均較正常對照組顯著增多(P均0.05)。結(jié)論:咬合支持喪失后腦組織海馬區(qū)細(xì)胞啟動了凋亡程序,并且與Bcl-2、Bax蛋白的表達(dá)存在相關(guān)性。
[Abstract]:Aim: to investigate the expression and correlation of apoptosis-related gene Bcl-2 (Bax) in hippocampus of rats with occlusal support loss. Methods: fifty four-week old SD rats were randomly divided into normal control group, three molar missing group, six molar missing group, nine molar missing group and twelve molar missing groups, each group had ten rats. The occlusal support loss group was fed for 20 weeks after extraction of the corresponding teeth, and then the hippocampus of the rat brain was removed. The morphological and structural changes of hippocampal cells in each group were observed by HE staining, and the expression of Bcl-2P Bax positive cells in hippocampus of each group was observed by immunohistochemical staining. Results: the morphology and structure of brain cells in normal control group were normal and no damage was found. There was no obvious change in the cells in the three molar loss groups, the cell layer decreased and the structure was disordered in the six molar missing groups, and the number of neurons in the hippocampal tissue decreased significantly in the nine molar absence groups, and the cell arrangement was loose. The number of nerve cells in the hippocampal tissue of the 12 molars missing group decreased sharply. The number of Bcl-2 positive cells in the hippocampus of the group with occlusal support loss was significantly lower than that in the control group, and the number of Bax positive cells in the hippocampus of the group with loss of occlusal support was significantly higher than that of the control group (P < 0.05). Conclusion: the apoptotic process was initiated in hippocampal cells after occlusal support loss and was correlated with the expression of Bcl-2 protein.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R780.2

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