本文選題：-脫氫膽固醇還原酶 + 腭突　； 參考：《實(shí)用口腔醫(yī)學(xué)雜志》2014年01期

【摘要】：目的:觀察7-脫氫膽固醇還原酶(Dhcr7)基因在小鼠胚胎腭突發(fā)育中表達(dá)量的變化。方法:在體視顯微鏡下取E13.5、E14.5和E15.5的小鼠胚胎的腭突,免疫組織化學(xué)(IHC)觀察Dhcr7蛋白在腭突中的表達(dá)定位,逆轉(zhuǎn)錄式聚合酶鏈?zhǔn)椒磻?yīng)(RT-PCR)法檢測Dhcr7 mRNA的表達(dá)變化,Western blot法檢測蛋白的表達(dá)變化。結(jié)果:Dhcr7主要表達(dá)在胚胎腭突間充質(zhì)細(xì)胞(EPM);E13.5、E14.5和E15.5 mRNA相對表達(dá)量(Dhcr7/β-actin)分別是0.692±0.084、0.595±0.051和0.369±0.061;E13.5與E14.5比較,P0.05;E13.5、E14.5分別與E15.5比較,P0.01;蛋白相對表達(dá)量(Dhcr7/GAPDH)分別是0.857±0.008、0.840±0.005和0.300±0.007,E13.5與E14.5相比,P0.05;E15.5分別與E13.5和E14.5相比P0.01。結(jié)論:Dhcr7在腭突發(fā)育中的關(guān)鍵階段有表達(dá),并且表達(dá)量與其生長發(fā)育有密切關(guān)系。
[Abstract]:Aim: to observe the expression of 7-dehydrocholesterol reductase (Dhcr7) gene in the development of mouse palatal process. Methods: the palatine process of mouse embryos of E13.5E14.5 and E15.5 were taken under stereoscopic microscope, and the expression of Dhcr7 protein in palatine process was observed by immunohistochemistry. Reverse transcriptase polymerase chain reaction (RT-PCR) was used to detect the expression of Dhcr7 mRNA and Western blot was used to detect the protein expression. 緇撴灉:Dhcr7涓昏琛ㄨ揪鍦ㄨ儦鑳庤叚紿侀棿鍏呰川緇嗚優(yōu)(EPM);E13.5,E14.5鍜孍15.5 mRNA鐩稿琛ㄨ揪閲，

本文編號(hào)：1944223