本文選題：酪酸 + 成骨　； 參考：《廣東醫(yī)學(xué)》2016年11期

【摘要】：目的研究酪酸調(diào)節(jié)大鼠成骨細胞系(ROS17/2.8)中骨唾液酸蛋白(BSP)轉(zhuǎn)錄的作用機制。方法使用能生成BSP的大鼠成骨細胞系(ROS17/2.8),分為試驗組和對照組。試驗組首先加入無血清α-基本必須培養(yǎng)基(α-MEM)配制的(10-8~10~(-2))mol/L濃度梯度的酪酸12 h,確定最優(yōu)刺激濃度為10~(-4)mol/L,然后使用10~(-4)mol/L的酪酸作用于ROS17/2.8細胞3、6、12 h,對照組加入等量不含酪酸的無血清α-MEM。用Northern雜交試驗檢測BSP mRNA的表達;用瞬時轉(zhuǎn)染方法檢測BSP啟動子的轉(zhuǎn)錄活性變化;用凝膠遷移實驗驗證酪酸對核蛋白與相關(guān)基因區(qū)段結(jié)合的作用。結(jié)果酪酸作用ROS17/2.8細胞12 h后BSP mRNA的表達水平升高。酪酸對BSP基因啟動子轉(zhuǎn)錄活性的上調(diào)作用主要通過啟動子p LUC3(-116~+60)中的纖維母細胞生長因子2響應(yīng)區(qū)段(FRE),在凝膠遷移試驗中,酪酸促進了FRE片段與相應(yīng)核蛋白的結(jié)合。結(jié)論酪酸通過BSP啟動子中的FRE片段增強BSP基因的轉(zhuǎn)錄,從而在成骨早期增強成骨細胞活性。
[Abstract]:Objective to study the mechanism of tyroic acid regulating the transcription of bone sialic acid protein (BSPs) in rat osteoblast cell line (ROS17 / 2.8). Methods the rat osteoblast cell line, ROS17 / 2.8, which could produce BSP, was divided into two groups: experimental group and control group. The experimental group was treated with serum-free 偽 -essential medium (偽 -MEM) for 12 h with a concentration gradient of 10 ~ (-8) ~ 10 ~ (-10) ~ 10 ~ (-1) O ~ (-1) mol 路L ~ (-1). The optimal concentration of tyroic acid was determined to be 10 ~ (4) 渭 mol / L. Then, the ROS17/2.8 cells were treated with 10~(-4)mol/L for 3 ~ 612 h. The control group was added a serum-free 偽 -MEM without butyric acid. The expression of BSP mRNA was detected by Northern hybridization assay, the transcriptional activity of BSP promoter was detected by transient transfection method, and the effect of casein on the binding of nucleoprotein to related gene regions was verified by gel migration assay. Results the expression of BSP mRNA in ROS17/2.8 cells increased 12 h after treatment with butyric acid. The upregulation of the transcriptional activity of BSP gene promoter by caseic acid was mainly through the FRE2 response region of promoter pLUC3- 116-60. In the gel migration test, casein promoted the binding of FRE fragment to the corresponding nucleoprotein. Conclusion tyroic acid enhances the transcription of BSP gene through the FRE fragment of BSP promoter, thus enhances the activity of osteoblasts in the early stage of osteogenesis.
【作者單位】： 天津市口腔醫(yī)院口腔頜面外科;山西省人民醫(yī)院口腔頜面外科;日本大學(xué)松戶齒學(xué)部;
【分類號】：R781.4

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