發(fā)布時(shí)間：2018-05-23 19:16

  本文選題：IL-23R + 基因多態(tài)性��； 參考：《吉林大學(xué)》2015年碩士論文

【摘要】：口腔扁平苔蘚(oral lichen planus，OLP)是常見的口腔黏膜疾病，病程遷延，不易治愈。是T細(xì)胞介導(dǎo)的慢性炎癥性疾病，CD4+T淋巴細(xì)胞的免疫異常在其發(fā)病中起關(guān)鍵作用，以上皮細(xì)胞不全角化、基底層液化變性以及固有層密集的淋巴細(xì)胞浸潤帶為病理特征。 IL-23是針對(duì)炎癥性疾病、自身免疫疾病和腫瘤研究的細(xì)胞因子[1]，作為IL-23信號(hào)傳遞因子的IL-23R是其復(fù)合物的兩個(gè)亞基之一，在2002年被首次報(bào)道后，逐漸引起人們的重視。相關(guān)文獻(xiàn)已報(bào)道了IL-23R在炎性疾病、自身免疫性疾病的發(fā)病機(jī)制以及惡性腫瘤的易感性里占重要地位，，但其在口腔扁平苔癬的研究幾近空白。 目的： 研究IL-23R基因多態(tài)性與口腔扁平苔癬的相關(guān)性。 方法： 采用聚合酶鏈反應(yīng)—限制性片段長度多態(tài)（PCR-RFLP）檢測(cè)66例口腔扁平苔癬患者（其中糜爛型21例）和70例健康對(duì)照者的IL-23R的兩個(gè)SNP位點(diǎn)（rs10889677和rs11465817），分析IL-23R基因多態(tài)性與口腔扁平苔癬的相關(guān)性。 結(jié)果： 1.IL-23R的rs10889677、 rs11465817兩個(gè)位點(diǎn)均檢測(cè)出AA、AC、CC三種基因型。 2.rs10889677、 rs11465817位點(diǎn)等位基因、基因型分布頻率在病例組和對(duì)照組間差異無統(tǒng)計(jì)學(xué)意義(P0.05)； 3.rs10889677位點(diǎn)基因型分布頻率在糜爛型與對(duì)照組比較差異有統(tǒng)計(jì)學(xué)意義(P=0.024)， CC+CA基因型個(gè)體發(fā)生糜爛型的風(fēng)險(xiǎn)是AA基因型的3.115倍(95%CI:1.135-8.548)。 結(jié)論： 1.受檢OLP人群IL-23R基因的兩個(gè)位點(diǎn)（rs10889677、rs11465817）均存在基因多態(tài)性。 2.IL-23R基因rs11465817位點(diǎn)SNP與OLP疾病易感性無明顯相關(guān)性。 3.rs10889677位點(diǎn)存在多態(tài)性變異，可能與OLP的疾病易感性和嚴(yán)重程度有關(guān)。
[Abstract]:Oral lichen planus (OLP) is a common oral mucosal disease. It is a chronic inflammatory disease mediated by T cells that the immune abnormality of CD4 T lymphocytes plays a key role in the pathogenesis of the disease. It is characterized by incomplete keratosis of epithelial cells liquefaction of the basal layer and dense lymphocytic infiltrating zone of the lamina propria. IL-23 is a cytokine for the study of inflammatory diseases, autoimmune diseases and tumors. IL-23R, as a signal transduction factor of IL-23, is one of the two subunits of its complex. After it was first reported in 2002, it has attracted more and more attention. It has been reported that IL-23R plays an important role in the pathogenesis of inflammatory diseases autoimmune diseases and the susceptibility of malignant tumors. However the study of IL-23R in oral lichen planus is almost blank. Objective: To study the association between IL-23R gene polymorphism and oral lichen planus. Methods: Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to detect two SNP loci of IL-23R in 66 patients with oral lichen planus (including 21 patients with erosive type) and 70 healthy controls. The polymorphism of IL-23R gene and oral cavity were analyzed. The association of lichen planus. Results: Rs10889677 of 1.IL-23R, three genotypes of ACCC were detected at two rs11465817 loci. 2. Rs10889677, the allele of rs11465817 locus and the frequency of genotype distribution had no significant difference between the case group and the control group (P 0.05). The frequency of genotype distribution of 3.rs10889677 locus in erosive type was significantly higher than that in control group (P 0.024). The risk of erosion of CC CA genotype individuals was 3.115 times that of AA genotype (95% CI: 1.135-8.548). Conclusion: 1. There were two loci of IL-23R gene in OLP population, rs10889677 rs11465817). SNP at rs11465817 site of 2.IL-23R gene had no significant correlation with susceptibility to OLP disease. The polymorphic variation of 3.rs10889677 locus may be related to the susceptibility and severity of OLP.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R781.5

【參考文獻(xiàn)】

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