本文選題：人細(xì)胞分裂控制蛋白 + c-Myc��； 參考：《重慶醫(yī)學(xué)》2014年31期

【摘要】：目的檢測(cè)人細(xì)胞分裂控制蛋白4(hCDC4)在口腔鱗狀細(xì)胞癌(OSCC)中的表達(dá)情況,分析其與臨床病理參數(shù)之間的相關(guān)性。方法收集52例手術(shù)切除的OSCC組織及12例正常組織,運(yùn)用免疫組織化學(xué)技術(shù)檢測(cè)hCDC4表達(dá)情況,分析hCDC4蛋白表達(dá)與臨床病理參數(shù)之間的相關(guān)性。應(yīng)用hCDC4-siRNA瞬時(shí)轉(zhuǎn)染OSCC細(xì)胞系Tca8113,MTT及蛋白免疫印跡(Western blot)檢測(cè)細(xì)胞增殖能力及c-Myc和Cyclin E蛋白表達(dá)變化。結(jié)果 hCDC4蛋白在正常組織中的表達(dá)顯著高于對(duì)應(yīng)的OSCC組織(83.3%vs.25.0%,P0.05);hCDC4蛋白低表達(dá)與較大腫瘤直徑(≥4cm)和高臨床分期(Ⅲ+Ⅳ)顯著相關(guān)(P0.05);敲低Tca8113細(xì)胞中hCDC4表達(dá),導(dǎo)致細(xì)胞增殖能力顯著增強(qiáng)(P0.05)以及c-Myc和Cyclin E蛋白蓄積。結(jié)論 hCDC4表達(dá)缺失導(dǎo)致c-Myc和Cyclin E蛋白蓄積可能是促進(jìn)腫瘤進(jìn)展的重要因素。
[Abstract]:Objective to detect the expression of human mitotic control protein 4hCDC4 in oral squamous cell carcinoma (OSCC) and to analyze its correlation with clinicopathological parameters. Methods Immunohistochemical technique was used to detect the expression of hCDC4 in 52 OSCC tissues and 12 normal tissues. The correlation between the expression of hCDC4 protein and clinicopathological parameters was analyzed. The cell proliferation and the expression of c-Myc and Cyclin E were detected by hCDC4-siRNA transient transfection into OSCC cell line Tca8113 and Western blot. Results the expression of hCDC4 protein in normal tissues was significantly higher than that in corresponding OSCC tissues (83.3vs.25.0). The low expression of hCDC4 protein was significantly correlated with the larger tumor diameter (鈮，

本文編號(hào)：1897530