本文選題：十肽 + 抗菌性能��； 參考：《華西口腔醫(yī)學雜志》2014年06期

【摘要】：目的評價人工合成抗菌肽(十肽)對口腔常見感染性疾病主要致病菌的抑菌活性。方法采用瓊脂擴散法及液體稀釋法體外評價十肽對變異鏈球菌、表兄鏈球菌、嗜酸乳桿菌、血鏈球菌、格氏鏈球菌、黏性放線菌、內氏放線菌、牙齦卟啉單胞菌、中間普雷沃菌、具核梭桿菌、伴放線嗜血桿菌及白色假絲酵母菌的抑菌性能,并測定十肽對變異鏈球菌的時間-殺菌曲線。結果十肽對所選實驗菌株均表現(xiàn)出不同的抑菌性能,對主要致齲菌的最小抑菌濃度MIC值為62.5~125μg·m L-1,而對主要牙周致病菌的MIC值為250~1 000μg·m L-1,其中,十肽對齲病主要致病菌變異鏈球菌有較強抑菌作用。時間-殺菌曲線結果顯示,十肽作用20 min后開始殺菌,30 min之后可完全殺滅細菌,且在24 h之內無細菌生長。結論新型人工合成抗菌肽十肽對口腔常見感染性疾病主要致病菌具有抑菌性能,其中對致齲變異鏈球菌的抑菌效果最為明顯。
[Abstract]:Objective to evaluate the antibacterial activity of synthetic antimicrobial peptides (CTPs) against the main pathogens of common oral infectious diseases. Methods Agar diffusion method and liquid dilution method were used to evaluate in vitro the effects of decapeptide on Streptococcus mutans, Streptococcus, Lactobacillus acidophilus, Streptococcus sanguis, Streptococcus gravis, Actinomyces viscosity, Actinomyces Neisseri and Porphyromonas gingivalis. The bacteriostatic properties of Prevobacter intermedia, Clostridium nucleatum, Haemophilus actinophilus and Candida albicans were determined, and the time-bactericidal curves of decapeptide against Streptococcus mutans were determined. Results the decapeptide showed different bacteriostatic properties against the selected strains. The minimum inhibitory concentration (MIC) was 62.5 ~ 125 渭 g mL ~ (-1) for the main cariogenic bacteria, while the MIC value for the main periodontal pathogenic bacteria was 250 ~ 1 000 渭 g / L ~ (-1). Decapeptide has strong bacteriostatic effect on Streptococcus mutans, the main pathogen of caries. The results of time-bactericidal curve showed that the decapeptide could kill the bacteria completely after 20 min treatment and no bacteria growth within 24 h after sterilization for 30 min. Conclusion the new synthetic antimicrobial peptide decapeptide has antimicrobial activity against the main pathogenic bacteria of common oral infectious diseases, among which the bacteriostasis effect is the most obvious for Streptococcus mutans caused by caries.
【作者單位】： 四川省醫(yī)學科學院·四川省人民醫(yī)院口腔科;大連醫(yī)科大學口腔醫(yī)院口腔內科;口腔疾病研究國家重點實驗室華西口腔醫(yī)院老年口腔科(四川大學);
【基金】：四川省科技支撐計劃基金資助項目(2008FZ0173)
【分類號】：R781.5

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