本文選題：羧甲基殼聚糖 + 溫敏凝膠�。� 參考：《鄭州大學(xué)學(xué)報(醫(yī)學(xué)版)》2014年05期

【摘要】：目的:觀察羧甲基殼聚糖(CMCS)溫敏凝膠對病變牙根面NIH3T3細胞附著和增殖的影響。方法:60顆患牙制成60個牙根片,分為5組。A組:超聲刮治+EDTA處理3 min+CMCS浸提液涂擦牙根表面5 min;B組:超聲刮治+EDTA處理3 min+無菌生理鹽水涂擦牙根表面5 min;C組:手動刮治+EDTA處理3 min+CMCS浸提液涂擦牙根表面5 min;D組:手動刮治+EDTA處理3 min+無菌生理鹽水涂擦牙根表面5 min;E組(對照組):EDTA處理3 min+無菌生理鹽水涂擦牙根表面5 min。將NIH3T3細胞與5組牙根片分別共培養(yǎng)16、24、72 h,采用細胞計數(shù)法和MTT法觀察結(jié)果。結(jié)果:培養(yǎng)16 h時,A、B、C、D組細胞計數(shù)均高于對照組。培養(yǎng)24、72 h時,A、B、C、D組細胞增殖能力均高于對照組,且使用CMCS浸提液的A組及C組的細胞增殖能力高于使用無菌生理鹽水的B組及D組(F=31.813和41.579,P0.05)。結(jié)論:采用手動或超聲進行牙周刮治及根面平整均有助于NIH3T3細胞的附著;聯(lián)合使用CMCS溫敏凝膠處理牙根表面可以促進NIH3T3細胞在牙根面的附著與增殖。
[Abstract]:Aim: to observe the effect of carboxymethyl chitosan (CMCs) thermosensitive gel on the attachment and proliferation of NIH3T3 cells. Methods Sixty affected teeth were made into 60 root slices. Divided into 5 groups. Group A: ultrasonic scraping EDTA treatment 3 min CMCS extraction solution smeared root surface 5 minutes B group: ultrasonic scraping EDTA 3 min sterile saline smeared root surface 5 minutes C group: manual scraping EDTA treatment 3 min CMCS extraction Group D was treated with EDTA for 3 min and treated with normal saline for 3 min. Group E (control group) was treated with sterile saline for 3 min, and the root surface was smeared with normal saline for 5 minutes. The NIH3T3 cells were co-cultured with 5 groups of teeth for 24 ~ 72 h, and the results were observed by cell count and MTT method. Results: after 16 hours of culture, the cell counts in group D were higher than those in control group. The proliferative ability of group D was higher than that of control group, and the proliferative ability of group A and C with CMCS extract was higher than that of group B and D with aseptic normal saline and group D (F31.813 and 41.579P0.05N). Conclusion: manual or ultrasonic periodontal curettage and root surface leveling are beneficial to the attachment of NIH3T3 cells and the treatment of root surface with CMCS thermo-sensitive gel can promote the adhesion and proliferation of NIH3T3 cells on the root surface.
【作者單位】： 河北聯(lián)合大學(xué)口腔醫(yī)學(xué)院;廈門市口腔醫(yī)院牙體牙髓病科;
【基金】：唐山市科技計劃項目13130283z 河北聯(lián)合大學(xué)科學(xué)研究基金項目z201144
【分類號】：R781.42

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