本文選題：根尖倒充填術 + 儲存方法��； 參考：《華北理工大學》2015年碩士論文

【摘要】：目的1比較不同的離體牙儲存方法及儲存時間對根尖微滲漏的影響,以期得出與新鮮離體牙實驗結果相近的儲存方法。2比較使用不同的根尖倒充填材料時,根管壁玷污層去除與否對根尖微滲漏的影響。方法1將收集的55顆新鮮單根管離體牙隨機分入:1)實驗組:采用4種不同的儲存方式(每組各10顆)分別是4℃蒸餾水、4℃10%甲醛溶液、4℃2%氯亞明溶液和-20℃冷凍儲存;2)對照組(5顆):新鮮離體牙(30分鐘內(nèi)用于實驗);3)陽性對照與陰性對照組(每組各5顆)。在儲存的第30天和第60天,隨機從實驗組抽取5個樣本用于實驗。所有樣本常規(guī)根管預備后,冷牙膠側向加壓法充填根管,根尖切除后進行根管倒預備,實驗組與對照組用GIC進行倒充填,去除根管上段充填物。所有樣本在牙根表面(除牙根橫斷面)均勻涂布2層透明指甲油,恒溫箱干燥。將樣本置于檢測微滲漏模型中。于第1、2、4、7、10、15、20天和30天檢測從冠方向根方漏出的葡萄糖的濃度。2對所收集的130顆單根管牙進行常規(guī)根管預備后,冷牙膠側向加壓法充填根管,根尖切除后進行根管倒預備,將樣本隨機分為實驗組和對照組(每組各65顆)。實驗組:用17%EDTA+1%氯亞明蕩洗根管;對照組:用5m L生理鹽水蕩洗根管。從實驗組和對照組中各隨機抽取5顆牙做掃描電鏡觀察。各組余下60顆分3組分別用MTA、GIC和銀汞合金進行倒充填,將根管內(nèi)上段充填物去除。所有樣本在牙根表面(除牙根橫斷面)均勻涂布2層透明指甲油,恒溫箱干燥。所有樣本置于微滲漏模型中,于第1、2、4、7、10、15、20天和30天檢測從冠方向根方漏出的葡萄糖的濃度。結果1陽性對照組在第1天可見乳膠管內(nèi)液體顯著下降;陰性對照組在觀察期間未檢測到葡萄糖的滲出。在實驗組和對照組中各時間點上均能檢測到不同程度的滲漏值。儲存30天與儲存60天測得的微滲漏值相比無顯著差異(P0.05)。所有實驗組中,-20℃冷凍儲存方法的滲漏值最小,與其他實驗組相比存在統(tǒng)計學差異(P0.05)。-20℃冷凍儲存與對照組(新鮮牙)相比,滲漏值變化不大,無統(tǒng)計學差異(P0.05)。2實驗組與對照組在各個時間點均能檢測到葡萄糖。在離體牙去除或者保留玷污層中,MTA與GIC、銀汞合金相比,MTA的滲漏值最小,而銀汞合金的滲漏值最大,其差異具有統(tǒng)計學意義(P0.05)。MTA和GIC進行倒充填時,去除根管壁玷污層較保留玷污層的滲透值小,此差異具有統(tǒng)計學意義(P0.05);而銀汞合金做倒充填時,去除與保留玷污層的滲透值相比無顯著差異(P0.05)。結論1不同儲存時間的離體牙用于根尖微滲漏實驗,對根尖微滲漏實驗不存在影響。在根尖微滲漏實驗中,-20℃冷凍儲存方法與新鮮離體牙所得實驗結果相近。2MTA用于根尖倒充填所引起的根尖微滲漏明顯低于GIC、銀汞合金。應用MTA、GIC做根尖倒充填時,去除根管壁玷污層較之保留對其根尖封閉性更好,而用銀汞合金做倒充填時玷污層去除與否對提高根尖封閉性無顯著意義。
[Abstract]:Objective 1 to compare the effect of different storage methods and storage time on the microleakage of the root apex, in order to obtain the effect of the storage method,.2, which is close to the results of the fresh tooth test, to compare the effect of the root canal wall smear layer on the root tip microleakage when using different apex filling materials. Method 1 55 fresh single root canals were collected. Body teeth were randomly divided into 1) experimental group: 4 different storage methods (10 of each group) were 4 degrees of distilled water, 4 centigrade 10% Formaldehyde Solution, 4 C 2% chloramine solution and -20 centigrade freezing storage; 2) the control group (5): fresh isolated teeth (in 30 minutes for experiment), 3) positive control and negative control group (each of each 5). 5 samples were selected randomly from the experimental group for the experiment. After the routine root canal preparation, the root canals were filled with cold tooth glue, and the root canal was prepared after the root apex resection. The experimental group and the control group filled the root canal with GIC to remove the upper part of the root canal. All samples were evenly coated on the root surface (except the root cross section) with 2 layers of transparency. The samples were placed in the microleakage model. At the 1,2,4,7,10,15,20 and 30 days, the concentration of glucose leaked from the root square of the crown was detected by.2. The root canals were filled with the cold tooth gum lateral pressure method and the root canal was prepared after the apex resection, and the samples were followed. The experimental group was divided into the experimental group and the control group (65 in each group). The experimental group was used to wash the root canal with 17%EDTA+1% chloramine, and the control group was washed with 5m L saline. The 5 teeth were randomly selected from the experimental group and the control group for scanning electron microscopy. The remaining 60 3 groups were filled with MTA, GIC and amalgam, and the upper part of the root canal was filled. All samples were evenly coated on the root surface (except the root cross section) with 2 layers of transparent nail oil and the constant temperature box was dry. All samples were placed in the microleakage model, and the concentration of glucose leaked from the root square was detected at day 1,2,4,7,10,15,20 and 30 days. The results of 1 positive pairs showed a significant drop in the emulsion in first days. The negative control group did not detect the exudation of glucose during the observation period. The leakage values of different degrees were detected at all time points in the experimental group and the control group. There was no significant difference between the 30 days storage and the 60 day storage microleakage (P0.05). In all experimental groups, the leakage value of the freezing storage method at -20 centigrade was the smallest, with the other experimental groups. Compared with the control group (fresh teeth), the leakage value changed little compared with the control group (P0.05). There was no statistical difference between the control group and the control group (P0.05). The.2 experiment group and the control group were able to detect the glucose at all time points. In the removal or retention of the stained layer, the MTA and GIC, the silver amalgam, the minimum leakage value of MTA, and the silver mercury. The leakage value of the alloy is the largest, and the difference has statistical significance (P0.05).MTA and GIC for backfilling, the removal of the smear layer of the root canal wall is smaller than that of the retaining layer. The difference has statistical significance (P0.05), but when the silver amalgam is filled, there is no significant difference (P0.05) between the removal and the retention of the smear layer (P0.05). Conclusion 1 different reservoirs are stored. In the experiment of root tip microleakage, the -20 centigrade cryopreservation method was similar to that of fresh fresh teeth, and the apex microleakage caused by.2MTA for root apex filling was significantly lower than that of GIC, and silver amalgam was used to fill the root tip with MTA and GIC. The removal of the smear layer of the root canal wall is better than that of the retention of the root canal, but the removal of the tarnished layer is not significant to the enhancement of the apex sealing ability when the amalgam is used for backfilling.

【學位授予單位】：華北理工大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R781.05

【共引文獻】

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2 王曉超;繆羽;;MTA的相關性能以及應用在牙體牙髓疾病治療中的研究進展[J];內(nèi)蒙古醫(yī)學雜志;2015年03期

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6 郭香君;不同沖洗液的抗菌性和玷污層清除效果的相關性研究[D];天津醫(yī)科大學;2014年

7 盛君曼;EDTA不同濃度和作用時間對根管壁及牙根抗折力影響的研究[D];天津醫(yī)科大學;2014年

8 王麗萍;牙本質小管方向對牙本質粘結強度的影響[D];南昌大學;2014年

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