本文選題：牙漂白 + 牙釉質(zhì)　； 參考：《北京大學(xué)學(xué)報(醫(yī)學(xué)版)》2014年01期

【摘要】：目的:觀察高濃度過氧化物美白劑對于釉質(zhì)表面變形鏈球菌生物膜的影響。方法:選擇拔除的離體牙,制備直徑5 mm的帶有天然釉質(zhì)表面的圓盤20個;分為兩組,其中10個圓盤用含有35%(質(zhì)量分?jǐn)?shù))過氧化氫凝膠(Beyond公司,美國)進行美白,另外10個為對照組。20個釉質(zhì)圓盤于滅菌人唾液中浸泡3.5 h形成獲得性膜,加入腦心浸液肉湯(brain heart infusion broth,BHI)和變形鏈球菌(UA 159菌株)混懸液,放入5%(體積分?jǐn)?shù))CO2孵箱進行孵育。在孵育3、7、14、21、28 d后,每組各取出2個圓盤,其中一個圓盤刮去釉質(zhì)表面的生物膜,系列稀釋,接種于腦心浸液瓊脂(BHI agar supplemented with blood,BHIS))血平皿菌落計數(shù);另一個圓盤以熒光染色后,用激光共聚焦顯微鏡(confocal laser scanning microscope,CLSM)分層掃描觀察生物膜。結(jié)果:培養(yǎng)3、7、14 d后美白牙面的生物膜內(nèi)活菌數(shù)均顯著少于對照組。在培養(yǎng)3 d后,美白釉質(zhì)表面生物膜內(nèi)活菌面積[(3 595±2 903)μm2vs.(89 155±65 963)μm2,t=8.71,P=0.00]和活菌比例[(26.0%±16.4%)vs.(92.2%±10.9%),t=19.93,P=0.00)]顯著低于對照組;但培養(yǎng)21 d后出現(xiàn)逆轉(zhuǎn):美白釉質(zhì)表面生物膜內(nèi)活菌面積[(66 262±23 772)μm2vs.(51 184±20 502)μm2,t=2.59,P=0.012]顯著超過對照組。傳統(tǒng)培養(yǎng)法得出的活菌數(shù)與CLSM結(jié)果一致。結(jié)論:美白后釉質(zhì)表面短期內(nèi)可抑制變形鏈球菌生物膜的生長,但在21 d后,美白組生物膜生長高于對照組。美白治療在短期內(nèi)有一定抗齲作用,但長期作用有待進一步研究。
[Abstract]:Objective: to observe the effect of high concentration peroxide whitening agent on Streptococcus mutans biofilm on enamel surface. Methods: 20 discs with natural enamel surface were prepared from extracted teeth, and were divided into two groups: 10 discs were whitened with 35% hydrogen peroxide gel beyond (USA). 20 enamel discs were immersed in sterilized human saliva for 3.5h to form acquired membrane, and the mixture of brain heart infusion brothi and Streptococcus mutans (UA159 strain) was added into the suspension and incubated in 5 (volume fraction of CO _ 2 incubator). After incubation for 28 days, 2 disks were removed in each group, one of which was scraped off the enamel surface biofilm, diluted by a series of dilution, inoculated with BHI agar supplemented with blood agar supplemented with BHISH) blood plate colony count, the other disc was stained with fluorescence, the other disk was stained with fluorescence. Confocal laser scanning microscopes were used to observe biofilm. Results: the number of living bacteria in the biofilm of the white teeth was significantly lower than that of the control group. After 3 days of culture, the living bacteria area [3 595 鹵2 903 渭 m2vs.(89 155 鹵65 963 渭 m] and the ratio of live bacteria [26.0% 鹵16.4vs.cu 92.2% 鹵10.9t 19.93P0. 00] were significantly lower than those of the control group. However, after 21 days of culture, the living bacteria area on the surface of enamel [66 262 鹵23 772 渭 m2vs.(51 184 鹵20 502 渭 m] 2.59 渭 m Pu 0.012 was significantly larger than that of the control group. The number of live bacteria obtained by traditional culture method was consistent with that of CLSM. Conclusion: the surface of enamel after whitening can inhibit the growth of Streptococcus mutans biofilm in a short period of time, but after 21 days, the growth of biofilm in whitening group is higher than that in control group. Whitening therapy has some anti-caries effect in short term, but the long-term effect needs further study.
【作者單位】： 北京大學(xué)口腔醫(yī)學(xué)院·口腔醫(yī)院牙體牙髓科;北京大學(xué)口腔醫(yī)學(xué)院·口腔醫(yī)院綜合科;
【基金】：北京大學(xué)口腔醫(yī)院歸國基金資助~~
【分類號】：R783.9

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本文編號：1841936