本文選題：氯化鋰 + 正畸性牙齒移動��； 參考：《吉林大學》2014年碩士論文

【摘要】：目的：檢測氯化鋰對大鼠正畸牙移動過程中牙根吸收和牙移動的影響，并探討將Wnt通路作為調控牙周組織改建靶點的可行性。 方法：選擇鼠齡在8周，體重約200g的成年健康雄性SD大鼠10只進行飼養(yǎng)。將大鼠隨機分為實驗組和對照組，每組各5只并建立正畸牙移動模型。即在全身麻醉條件下，在上頜中切牙和上頜右側第一磨牙之間放置鎳鈦拉簧，以上頜中切牙為支抗拉第一磨牙向近中，彈簧施加的力值為50g。實驗組大鼠每2天通過灌胃法按200mg/kg劑量給予LiCl溶液，對照組給予同等劑量的NaCl溶液。在加力14天后脫臼法處死大鼠，分離第一、二磨牙及其牙周組織，修剪后進行后續(xù)檢測。首先用Micro-CT對組織塊進行三維掃描并進行圖像重建，隨后通過軟件分析第一二磨牙牙冠最突點之間的距離；在完成Micro-CT的拍攝后，將組織塊進行修整，并在5%的次氯酸鈉溶液中浸泡12小時，小心的剝離第一磨牙牙根，并盡量擦去周圍軟組織。待牙根表面干燥后，，進行掃描電鏡拍攝，重點分析遠中頰側根和遠中腭側根，通過Image J軟件計算上述兩根近中面的吸收面積比率；最后通過spss軟件進行統(tǒng)計分析。 結果：實驗組和對照組大鼠第一磨牙和第二磨牙之間均有間隙出現(xiàn)，實驗組大鼠第一磨牙和第二磨牙之間的距離略大于對照組，但是二者并無顯著統(tǒng)計學差異（P=0.224）。實驗組和對照組大鼠第一磨牙遠中腭側根和遠中頰根近中面都觀察到了不同程度的牙根吸收。實驗組大鼠牙根吸收比率小于對照組，且二者間有顯著統(tǒng)計學差異(P0.05)。 結論：通過本實驗可以得出，氯化鋰能夠減輕正畸性牙齒移動過程中牙根吸收的嚴重程度，而對牙齒移動無顯著的影響。
[Abstract]:Aim: to investigate the effects of lithium chloride on root resorption and tooth movement during orthodontic tooth movement in rats, and to explore the feasibility of using Wnt pathway as a target for regulating periodontal tissue remodeling. Methods: ten adult male SD rats aged 8 weeks and weighing about 200 g were selected for feeding. Rats were randomly divided into experimental group and control group with 5 rats in each group and established orthodontic tooth movement model. Under general anesthesia, a nickel titanium pull spring was placed between the maxillary central incisor and the right first molar of the maxilla. The maxillary central incisor was used as the Anchorage to pull the first molar to the near center, and the force applied by the spring was 50 g. Rats in the experimental group were given LiCl solution at the dose of 200mg/kg every 2 days, while the rats in the control group were given the same dose of NaCl solution. The first and second molars and their periodontal tissues were isolated and pruned for subsequent detection. First, the tissue block was scanned and reconstructed by Micro-CT, and then the distance between the most protruding points of the first and second molars was analyzed by software. Soaking in 5% sodium hypochlorite solution for 12 hours, carefully stripping the root of the first molar and erasing the surrounding soft tissue as much as possible. After the root surface was dried, scanning electron microscopy was used to analyze the distal buccal lateral root and distal palatal lateral root. The absorptive area ratio of the two proximal midplane was calculated by Image J software. Finally, the statistical analysis was carried out by spss software. Results: there was a gap between the first molar and the second molar in the experimental group and the control group. The distance between the first molar and the second molar in the experimental group was slightly larger than that in the control group, but there was no significant difference between the two groups. Different degrees of root resorption were observed in the distal palatine lateral root and distal buccal root of the first molar in the experimental group and the control group. The ratio of root resorption in the experimental group was lower than that in the control group, and there was a significant difference between the two groups (P 0.05). Conclusion: lithium chloride can reduce the severity of root resorption during orthodontic tooth movement, but has no significant effect on tooth movement.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R783.5

【參考文獻】

相關期刊論文 前1條

1 牛銀波;李宇華;伍煥杰;李倩;孫陽;崔猛;王婷梅;梅其炳;;氯化鋰對去卵巢大鼠骨密度的影響研究[J];解放軍醫(yī)學雜志;2010年01期

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