本文選題：牙齦卟啉單胞菌 + 小鼠��； 參考：《北京大學(xué)學(xué)報(醫(yī)學(xué)版)》2017年01期

【摘要】：目的:比較絲線結(jié)扎和涂抹牙齦卟啉單胞菌誘導(dǎo)小鼠牙周炎模型的牙槽骨骨吸收程度、激活破骨細胞及去除誘因后成骨的過程。方法:48只C57BL6小鼠隨機分配,采用分口設(shè)計,根據(jù)涂抹2%(質(zhì)量分數(shù))羧甲基纖維素(carboxymethylcellulose,CMC)或10~9個菌落形成單位(colony-forming units,CFU)的牙齦卟啉單胞菌以及是否絲線結(jié)扎分為4組(n=24),分別為對照組(右上第二磨牙單純涂抹CMC)、絲線結(jié)扎組(圍繞左上第二磨牙結(jié)扎9-0絲線)、涂菌組(右上第二磨牙單純涂抹牙齦卟啉單胞菌)和絲線結(jié)扎+涂菌組(結(jié)扎左上第二磨牙并涂抹牙齦卟啉單胞菌),測量釉牙骨質(zhì)界至牙槽骨嵴頂(cementoenamel junction to the alveolar bone crest,CEJ-ABC)的距離,監(jiān)測牙槽骨的吸收。48只C57BL6小鼠設(shè)計和分組同上,牙槽骨骨面的破骨細胞采用抗酒石酸酸性磷酸酶(tartrateresistant acid phosphatase,TRAP)染色并計數(shù)。36只C57BL6小鼠隨機分配,其中30只小鼠在左側(cè)上頜第二磨牙結(jié)扎9-0絲線,觀察3周,有12只小鼠在絲線結(jié)扎1周后拆除絲線,繼續(xù)觀察2周,分別在絲線結(jié)扎1、2、3周以及絲線拆除1周和2周共5個時間點(n=6)各處死6只小鼠,檢測CEJ-ABC距離;未行絲線結(jié)扎的小鼠CEJ-ABC距離(n=6)為基線水平。采用單因素方差分析(ANOVA)法對所有數(shù)據(jù)進行統(tǒng)計學(xué)分析。結(jié)果:絲線結(jié)扎組的小鼠牙周炎,在3、6、9和12周后的CEJ-ABC距離分別為(0.16±0.04)mm、(0.16±0.02)mm、(0.18±0.03)mm、(0.17±0.02)mm,與對照組[(0.09±0.03)mm、(0.10±0.01)mm、(0.12±0.04)mm、(0.12±0.01)mm]和涂菌組[(0.09±0.03)mm、(0.12±0.01)mm、(0.12±0.02)mm、(0.10±0.01)mm]相比差異有統(tǒng)計學(xué)意義(P0.05)。與對照組[(0.09±0.03)mm]相比,絲線結(jié)扎組第3周的CEJ-ABC距離為(0.16±0.04)mm,比涂菌組[(0.09±0.03)mm]誘導(dǎo)的牙槽骨骨吸收更迅速,絲線結(jié)扎和涂抹牙齦卟啉單胞菌結(jié)合并未進一步增加牙槽骨的吸收破壞。在絲線結(jié)扎1 d后,牙槽骨表面的破骨細胞即被激活,與對照組[(2±2)個]相比在第3天達到高峰[(12±4)個,P0.01]。絲線結(jié)扎誘導(dǎo)的小鼠牙周炎,在絲線去除2周后CEJ-ABC距離為(0.07±0.02)mm,與去除絲線前[(0.13±0.01)mm]相比顯著降低,提示有顯著的牙槽骨再生(P0.01)。結(jié)論:絲線結(jié)扎術(shù)是一種迅速且有效的誘導(dǎo)小鼠牙周炎牙槽骨骨吸收的方式,其誘導(dǎo)的破骨細胞活化在絲線結(jié)扎后24 h內(nèi)發(fā)生,3 d達到高峰,去除實驗性牙周炎的誘導(dǎo)因素即去除結(jié)扎的絲線有助于牙槽骨骨再生。
[Abstract]:Aim: to compare the degree of alveolar bone resorption, the activation of osteoclasts and the osteogenesis after removing the inducement of porphyromonas gingivalis in mouse periodontitis model induced by ligation of filaments and smear of porphyromonas gingivalis. Methods A total of 48 C57BL6 mice were randomly assigned. Porphyromonas gingivalis smeared with carboxymethylcellulose carboxymethylcellulosec (CMCC) or 109 colony forming units (CFU) and whether or not filaments were ligated were divided into 4 groups: control group (right second molars coated with CMCC alone, filamentous knot). Group B (ligation of 9-0 filaments around left second molar, smear group (right upper second molar) and filaments ligation group (ligation of left upper second molar and smear of porphyromonas gingivalis) were measured. The distance between the cementum boundary of the enamel and the crest of the alveolar crest, the distance between enamel junction to the alveolar bone crestCase (ABJ-ABC), The absorption of alveolar bone was monitored in 48 C57BL6 mice. The osteoclasts on the alveolar bone surface were stained with tartrateresistant acid phosphataseTRAPAPs and counted. 36 C57BL6 mice were randomly assigned. The 9-0 thread was ligated in the left maxillary second molar in 30 mice. For 3 weeks, 12 mice removed the silk thread after 1 week of ligation and continued to observe for 2 weeks. Six mice were killed at 3 weeks after ligation of silk thread and 1 week and 2 weeks after removal of silk thread. The CEJ-ABC distance was detected in each group, and the CEJ-ABC distance of mice without ligation of silk thread was determined as baseline level. All the data were statistically analyzed by ANOVA (single factor ANOVA) method. Compared with the control group [0.09 鹵0.03)mm], the CEJ-ABC distance of the filaments ligation group was 0.16 鹵0.04mm at the 3rd week, which was faster than the alveolar bone resorption induced by the smear bacteria group [0.09 鹵0.03)mm], and the absorption damage of alveolar bone was not further increased by the ligation of filaments and the application of porphyromonas gingivalis. The osteoclasts on the surface of alveolar bone were activated 1 day after the ligation of the filaments, and reached a peak on the 3rd day compared with the control group [2 鹵2] [12 鹵4] (P0.01). The CEJ-ABC distance of periodontitis induced by ligation of filaments in mice was 0.07 鹵0.02mm. the distance of CEJ-ABC was 0.07 鹵0.02mm. it was significantly lower than that before filamentral ligation [0.13 鹵0.01)mm], indicating that there was significant alveolar bone regeneration (P0.01). Conclusion: the ligation of filaments is a rapid and effective way to induce alveolar bone resorption in mice with periodontitis. The activation of osteoclasts reached its peak within 24 hours after ligation of filaments. Removal of experimental periodontitis induces removal of ligated filaments to facilitate alveolar bone regeneration.
【作者單位】： 北京大學(xué)口腔醫(yī)學(xué)院·口腔醫(yī)院牙周科口腔數(shù)字化醫(yī)療技術(shù)和材料國家工程實驗室口腔數(shù)字醫(yī)學(xué)北京市重點實驗室;
【基金】：國家自然科學(xué)基金青年基金(81000440) 教育部留學(xué)回國人員科研啟動基金項目資助~~
【分類號】：R781.4

【相似文獻】

相關(guān)期刊論文 前10條

1 林龍英;李捷;王家興;何永;馬良;蒲琦;;兩孔絲線結(jié)扎法腹腔鏡膽囊切除術(shù)的臨床應(yīng)用[J];中國微創(chuàng)外科雜志;2010年05期

2 范吉利;黃和邦;商志遠;;腔內(nèi)絲線結(jié)扎法腹腔鏡闌尾切除術(shù)臨床分析[J];中國內(nèi)鏡雜志;2008年12期

3 李延豹;牟新東;杜洪超;;絲線結(jié)扎法行腹腔鏡闌尾切除術(shù)的臨床應(yīng)用研究[J];大家健康(學(xué)術(shù)版);2013年20期

4 徐紅方;陸紅全;方登峰;張大宏;;絲線結(jié)扎法腹腔鏡精索靜脈結(jié)扎術(shù)[J];浙江醫(yī)學(xué);2007年10期

5 蔣新德;陳學(xué)榮;;腹腔鏡膽囊切除術(shù)中絲線結(jié)扎56例臨床分析[J];肝膽胰外科雜志;2010年04期

6 李元紅 ,歐陽時婕 ,曲健;絲線結(jié)扎代替鈦夾在腹腔鏡手術(shù)中應(yīng)用41例[J];腹腔鏡外科雜志;2004年01期

7 易先武;徐其銀;樊建新;黃君;;絲線結(jié)扎闌尾及系膜在腹腔鏡闌尾切除術(shù)中的臨床應(yīng)用分析[J];中國醫(yī)藥科學(xué);2013年03期

8 程云霄,呂忠,葛敦鈞;LC術(shù)中絲線結(jié)扎應(yīng)用體會[J];中國微創(chuàng)外科雜志;2001年04期

9 楊媛媛;牛剛;丁憲群;;腹腔鏡膽囊切除術(shù)中改良絲線結(jié)扎膽囊動脈、膽囊管80例的臨床觀察[J];貴陽中醫(yī)學(xué)院學(xué)報;2013年05期

10 渠時學(xué);孫峰;丁碩;李青;宋瑞麗;;應(yīng)用腹腔鏡多功能夾持鉗施行腹腔鏡膽囊切除術(shù)105例報告[J];肝膽胰外科雜志;2008年02期

，

本文編號：1787910