本文選題：下齒槽神經(jīng) + 損傷�。� 參考：《遼寧醫(yī)學(xué)院》2014年碩士論文

【摘要】：目的 通過建立口腔種植體植入術(shù)中下齒槽神經(jīng)損傷的動物實(shí)驗(yàn)?zāi)Ｐ�，局部�?yīng)用神經(jīng)營養(yǎng)因子（NGF），促進(jìn)種植手術(shù)過程中受損的下齒槽神經(jīng)功能的恢復(fù)，從而為種植術(shù)中下齒槽神經(jīng)損傷后的系統(tǒng)性治療開辟一個新的思路。 方法 建立犬口腔種植術(shù)后下齒槽神經(jīng)損傷動物實(shí)驗(yàn)?zāi)Ｐ�，�?shí)驗(yàn)組于損傷后局部給予NGF微球，對照組給予空白微球，空白組不損傷神經(jīng)，于1d、3d、7d、4w取神經(jīng)損傷處遠(yuǎn)段制作標(biāo)本，應(yīng)用免疫熒光、Western Blot方法觀察標(biāo)本中NF-kappaB、GAP43的表達(dá)及神經(jīng)生長情況。 結(jié)果 術(shù)后各組犬均成活，精神狀態(tài)、飲食、活動正常，切口愈合良好，免疫熒光結(jié)果為空白組NF-kB陽性表達(dá)率低；對照側(cè)術(shù)后1至3天后陽性表達(dá)率逐漸增加，7天時陽性表達(dá)率最高，至術(shù)后4周陽性表達(dá)率有所降低；實(shí)驗(yàn)側(cè)術(shù)后1天后陽性表達(dá)率即增加，3天陽性表達(dá)率最高，并于7天至4周后陽性表達(dá)率逐漸下降。對照側(cè)術(shù)后1至3天陽性表達(dá)率逐漸增加，7天陽性表達(dá)率最高，至4周后陽性表達(dá)率逐漸減弱；實(shí)驗(yàn)側(cè)術(shù)后1到3天陽性表達(dá)率達(dá)到高峰，7天至4周陽性表達(dá)率迅速減弱。Western Blot結(jié)果顯示NF-kB在空白組極少量表達(dá)；對照側(cè)在術(shù)后1至3天顯著表達(dá)，在7天達(dá)到峰值，，此后表達(dá)迅速下降，表達(dá)量均大于空白組；實(shí)驗(yàn)側(cè)術(shù)后顯著表達(dá)，3天達(dá)到峰值，此后有所下降，表達(dá)量均大于空白組。GAP43在空白組極少量表達(dá)；對照側(cè)在術(shù)后1天和3天表達(dá)呈上升趨勢，7天達(dá)到峰值，此后迅速下降，表達(dá)量均大于空白組；實(shí)驗(yàn)側(cè)術(shù)后表達(dá)顯著，3天達(dá)峰值，此后表達(dá)緩慢下降，表達(dá)量均大于空白組。 結(jié)論 神經(jīng)生長因子緩釋劑可為一種良好的修復(fù)周圍神經(jīng)損傷的蛋白，其臨床前景廣闊。
[Abstract]:Purpose By establishing the animal model of the injury of the inferior alveolar nerve in the dental implant implantation, the neurotrophic factor NGF was used locally to promote the recovery of the damaged inferior alveolar nerve function during the implant operation. Thus, a new idea for systematic treatment of inferior alveolar nerve injury in implant operation is opened up. Method The experimental model of the injury of the inferior alveolar nerve was established. The experimental group was given NGF microspheres locally after the injury, the control group was given the blank microsphere, the blank group did not injure the nerve, and the distal segment of the injured nerve was taken at 1 day, 3 days, 7 days and 4 weeks after the injury. The expression of NF-kappa Bon GAP43 and nerve growth were observed by Western Blot. Result All the dogs survived, mental state, diet, activity were normal, wound healing was good, the positive expression rate of NF-kB in the blank group was low, the positive expression rate of NF-kB in the control group increased gradually after 1 to 3 days after operation, the positive expression rate was the highest at 7 days after operation. The positive expression rate decreased at 4 weeks after operation, and the positive expression rate increased at 3 days after operation on the experimental side, and decreased gradually from 7 days to 4 weeks after operation. The positive expression rate of the control side increased gradually from 1 to 3 days after operation, the highest positive expression rate was on the 7th day, and the positive expression rate gradually decreased after 4 weeks. The positive expression rate of NF-kB decreased rapidly from 7 days to 4 weeks after operation on the experimental side. The results of Western Blot showed that the expression of NF-kB was very small in the blank group, while the positive expression rate in the control side reached the peak on the 1st to 3rd day after operation and reached the peak on the 7th day. After that, the expression of GAP43 decreased rapidly, and the expression of GAP43 was higher than that of the blank group, and the expression of GAP43 reached the peak 3 days after operation, and the expression of GAP43 was lower than that of the blank group. On the 1st and 3rd day after operation, the expression of the control side reached its peak value on the 1st and 3rd day after operation, and then decreased rapidly, and the expression level was higher than that in the blank group, and the expression reached the peak on the 3rd day after operation, and then the expression decreased slowly, and the expression was higher than that in the blank group. Conclusion Nerve growth factor (NGF) sustained-release agent is a good protein for repairing peripheral nerve injury.
【學(xué)位授予單位】：遼寧醫(yī)學(xué)院
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R782.12

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 曾晗冰;徐華梓;李萬里;李士;;改良復(fù)乳法制備神經(jīng)生長因子微球及其體外釋藥性能[J];中國組織工程研究與臨床康復(fù);2009年42期

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