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  本文選題：下齒槽神經 + 損傷��； 參考：《遼寧醫(yī)學院》2014年碩士論文

【摘要】：目的 通過建立口腔種植體植入術中下齒槽神經損傷的動物實驗模型，局部應用神經營養(yǎng)因子（NGF），促進種植手術過程中受損的下齒槽神經功能的恢復，從而為種植術中下齒槽神經損傷后的系統(tǒng)性治療開辟一個新的思路。 方法 建立犬口腔種植術后下齒槽神經損傷動物實驗模型，實驗組于損傷后局部給予NGF微球，對照組給予空白微球，空白組不損傷神經，于1d、3d、7d、4w取神經損傷處遠段制作標本，應用免疫熒光、Western Blot方法觀察標本中NF-kappaB、GAP43的表達及神經生長情況。 結果 術后各組犬均成活，精神狀態(tài)、飲食、活動正常，切口愈合良好，免疫熒光結果為空白組NF-kB陽性表達率低；對照側術后1至3天后陽性表達率逐漸增加，7天時陽性表達率最高，至術后4周陽性表達率有所降低；實驗側術后1天后陽性表達率即增加，3天陽性表達率最高，并于7天至4周后陽性表達率逐漸下降。對照側術后1至3天陽性表達率逐漸增加，7天陽性表達率最高，至4周后陽性表達率逐漸減弱；實驗側術后1到3天陽性表達率達到高峰，7天至4周陽性表達率迅速減弱。Western Blot結果顯示NF-kB在空白組極少量表達；對照側在術后1至3天顯著表達，在7天達到峰值，，此后表達迅速下降，表達量均大于空白組；實驗側術后顯著表達，3天達到峰值，此后有所下降，表達量均大于空白組。GAP43在空白組極少量表達；對照側在術后1天和3天表達呈上升趨勢，7天達到峰值，此后迅速下降，表達量均大于空白組；實驗側術后表達顯著，3天達峰值，此后表達緩慢下降，表達量均大于空白組。 結論 神經生長因子緩釋劑可為一種良好的修復周圍神經損傷的蛋白，其臨床前景廣闊。
[Abstract]:Purpose By establishing the animal model of the injury of the inferior alveolar nerve in the dental implant implantation, the neurotrophic factor NGF was used locally to promote the recovery of the damaged inferior alveolar nerve function during the implant operation. Thus, a new idea for systematic treatment of inferior alveolar nerve injury in implant operation is opened up. Method The experimental model of the injury of the inferior alveolar nerve was established. The experimental group was given NGF microspheres locally after the injury, the control group was given the blank microsphere, the blank group did not injure the nerve, and the distal segment of the injured nerve was taken at 1 day, 3 days, 7 days and 4 weeks after the injury. The expression of NF-kappa Bon GAP43 and nerve growth were observed by Western Blot. Result All the dogs survived, mental state, diet, activity were normal, wound healing was good, the positive expression rate of NF-kB in the blank group was low, the positive expression rate of NF-kB in the control group increased gradually after 1 to 3 days after operation, the positive expression rate was the highest at 7 days after operation. The positive expression rate decreased at 4 weeks after operation, and the positive expression rate increased at 3 days after operation on the experimental side, and decreased gradually from 7 days to 4 weeks after operation. The positive expression rate of the control side increased gradually from 1 to 3 days after operation, the highest positive expression rate was on the 7th day, and the positive expression rate gradually decreased after 4 weeks. The positive expression rate of NF-kB decreased rapidly from 7 days to 4 weeks after operation on the experimental side. The results of Western Blot showed that the expression of NF-kB was very small in the blank group, while the positive expression rate in the control side reached the peak on the 1st to 3rd day after operation and reached the peak on the 7th day. After that, the expression of GAP43 decreased rapidly, and the expression of GAP43 was higher than that of the blank group, and the expression of GAP43 reached the peak 3 days after operation, and the expression of GAP43 was lower than that of the blank group. On the 1st and 3rd day after operation, the expression of the control side reached its peak value on the 1st and 3rd day after operation, and then decreased rapidly, and the expression level was higher than that in the blank group, and the expression reached the peak on the 3rd day after operation, and then the expression decreased slowly, and the expression was higher than that in the blank group. Conclusion Nerve growth factor (NGF) sustained-release agent is a good protein for repairing peripheral nerve injury.
【學位授予單位】：遼寧醫(yī)學院
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R782.12

【參考文獻】

相關期刊論文 前1條

1 曾晗冰;徐華梓;李萬里;李士;;改良復乳法制備神經生長因子微球及其體外釋藥性能[J];中國組織工程研究與臨床康復;2009年42期

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