本文選題：交感神經(jīng) + 失交感神經(jīng)支配��； 參考：《福建醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的： 骨的新陳代謝受到神經(jīng)系統(tǒng)支配的影響。目前大量的研究表明神經(jīng)系統(tǒng)，特別是交感神經(jīng)系統(tǒng)參與對骨改建的調(diào)控。其調(diào)控的媒介為成骨細(xì)胞和破骨細(xì)胞。本實驗通過外科手術(shù)的方法創(chuàng)建失交感神經(jīng)支配的大鼠模型，研究骨改建中交感神經(jīng)對破骨細(xì)胞生理功能的調(diào)節(jié)作用。證明失交感神經(jīng)支配可以影響破骨細(xì)胞的生理功能進(jìn)而影響骨改建，為臨床應(yīng)用提供實驗依據(jù)。 材料和方法： 1、利用外科手術(shù)方法將大鼠單側(cè)頸上神經(jīng)節(jié)摘除，創(chuàng)建大鼠失交感神經(jīng)支配的動物模型。2、拔除大鼠實驗側(cè)（失交感神經(jīng)支配側(cè)）與對照側(cè)（交感神經(jīng)支配側(cè)）上頜第一磨牙，創(chuàng)建拔牙窩骨愈合的動物模型。3、分別于拔牙術(shù)后01天、03天、07天、14天和21天5個時間點處死動物，進(jìn)行TRAP陽性染色破骨細(xì)胞計數(shù)及RANKL，OPG蛋白免疫組化染色。 結(jié)果： 1、 TRAP染色檢測結(jié)果表明：拔牙窩在各個時間點均顯示實驗組（失交感神經(jīng)支配組）破骨細(xì)胞數(shù)目少于對照組（交感神經(jīng)支配組），在03天、07天和14天時,實驗組與對照組相比存在統(tǒng)計學(xué)差異(P0.01)。 2、 RANKL，OPG蛋白免疫組化染色的組織學(xué)觀察結(jié)果表明：（1）、大鼠拔牙窩RANKL的平均光密度值在各個時間點實驗組均小于對照組，01天時實驗組與對照組相比無統(tǒng)計學(xué)差異（P0.05);在03天、07天、14天、21天時實驗組與對照組相比存在統(tǒng)計學(xué)差異（P0.01）。（2）、大鼠OPG的平均光密度值在拔牙窩內(nèi)陽性表達(dá)隨著拔牙時間的增加而增強;在各個時間點實驗組均大于對照組；在01天、03天、07天、14天、21天時實驗組與對照組相比均存在統(tǒng)計學(xué)差異（P0.01）。（3）、大鼠拔牙窩內(nèi)RANKL/OPG含量比值在各個時間點實驗組均小于對照組，在01天、03天、07天、14天、21天時實驗組與對照組相比均存在統(tǒng)計學(xué)差異（P0.01）。 結(jié)論： 本實驗結(jié)果表明利用外科手術(shù)方法建立的失交感神經(jīng)支配能有效地抑制頜骨骨吸收，使破骨細(xì)胞數(shù)目明顯減少，破骨細(xì)胞活性明顯降低，骨吸收減少，骨形成增加，從而證實了交感神經(jīng)系統(tǒng)確實參與了對頜骨骨改建的調(diào)控，，交感神經(jīng)系統(tǒng)調(diào)控頜骨骨改建的機制可能是通過影響破骨細(xì)胞的數(shù)量和活性來完成的。
[Abstract]:Objective:The metabolism of bone is influenced by the nervous system.A large number of studies have shown that the nervous system, especially the sympathetic nervous system, is involved in the regulation of bone remodeling.Osteoblasts and osteoclasts are the mediators of its regulation.In this study, a rat model of denervated sympathetic nerve was established by surgical operation to study the effect of sympathetic nerve on the physiological function of osteoclasts in bone remodeling.It is proved that denervation can affect the physiological function of osteoclasts and bone remodeling, which provides experimental basis for clinical application.Materials and methods:1. The unilateral superior cervical ganglion was removed by surgical method, and the animal model of denervated sympathetic nerve was established. The experimental side (the anaesthetized side) and the control side (the sympathetic innervation side) were extracted from the maxillary first molar.The animal model of bone healing was established. The animals were killed at 01d, 03d, 07d, 14d and 21d, respectively. The osteoclast count of TRAP positive staining and the immunohistochemical staining of RANKL-OPG protein were performed.Results:1. The results of TRAP staining showed that the number of osteoclasts in the experimental group was lower than that in the control group at all time points, and the number of osteoclasts in the experimental group was lower than that in the control group.There was statistical difference between the experimental group and the control group (P 0.01).2. The histological observation of RANKL OPG protein immunohistochemical staining showed that the average optical density of RANKL in the tooth extraction fossa of rats was lower than that of the control group at every time point, and there was no significant difference between the experimental group and the control group on the 01st day, and the difference was not significant between the experimental group and the control group on the 03rd day (P 0.05).There was a statistical difference between the experimental group and the control group at 14 days and 21 days. The average optical density of OPG in the tooth extraction fossa increased with the increase of extraction time.At each time point, the experimental group was larger than the control group, and the experimental group was significantly different from the control group on the 1st day, 03rd day, 07 day, 14 day and 21 day, and the ratio of RANKL/OPG content in the tooth extraction fossa was smaller in the experimental group than that in the control group at each time point, and the ratio of RANKL/OPG content in the experimental group was lower than that in the control group at each time point.There was a statistical difference between the experimental group and the control group at 01 days, 03 days, 07 days, 14 days and 21 days, and there was a significant difference between the experimental group and the control group (P 0.01).Conclusion:The results showed that the aposymphatic innervation established by surgical method could effectively inhibit the resorption of jaw bone, decrease the number of osteoclasts, decrease the activity of osteoclasts, decrease bone resorption and increase bone formation.It is proved that the sympathetic nervous system is involved in the regulation of jaw bone remodeling, and the mechanism of sympathetic nerve system regulating the reconstruction of jaw bone may be accomplished by affecting the number and activity of osteoclasts.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R782.1

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相關(guān)期刊論文 前2條

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