本文選題：脂肪間充質(zhì)干細胞 + 多向分化　； 參考：《口腔醫(yī)學(xué)研究》2017年08期

【摘要】：目的:進行脂肪干細胞的分離、培養(yǎng)和鑒定,闡明脂肪干細胞的生物學(xué)特性。方法:無菌條件下取新西蘭大耳白兔腹股溝處脂肪組織,從中獲得純化的脂肪來源干細胞。HE染色觀察細胞形態(tài);流式細胞術(shù)檢測細胞表面標(biāo)志物;多向誘導(dǎo)后檢測其成脂、成骨及成軟骨分化能力。結(jié)果:提取的兔脂肪干細胞形態(tài)為成纖維細胞樣,生長旺盛,可以穩(wěn)定增殖20代以上。流式細胞術(shù)分析顯示CD44呈陽性表達,CD34、CD14呈陰性表達;經(jīng)體外誘導(dǎo)培養(yǎng)后,向成脂細胞、成骨細胞及成軟骨細胞分化。結(jié)論:本實驗成功分離、培養(yǎng)和鑒定了脂肪干細胞,脂肪干細胞體外增殖穩(wěn)定,并且具有成脂、成骨及成軟骨多向分化潛能。
[Abstract]:Objective: to isolate, culture and identify adipose stem cells and elucidate the biological characteristics of adipose stem cells.Methods: adipose tissue in inguinal region of New Zealand white rabbit was harvested under aseptic condition and purified adipose-derived stem cells were obtained. He staining was used to observe cell morphology, flow cytometry was used to detect cell surface markers, and lipid production was detected after multi-directional induction.Ability of osteogenesis and cartilage differentiation.Results: the extracted adipose stem cells were fibroblast-like and could proliferate steadily for more than 20 generations.Flow cytometry showed that the expression of CD44 was positive and CD34 + CD14 was negative, and differentiated into adipoblasts, osteoblasts and chondroblasts after induction and culture in vitro.Conclusion: adipose stem cells were isolated, cultured and identified successfully in this experiment. Adipose stem cells proliferate stably in vitro and have the potential of adipogenic, osteogenic and cartilage differentiation.
【作者單位】： 吉林大學(xué)口腔醫(yī)院口腔頜面外科;吉林大學(xué)口腔醫(yī)院正畸科;
【基金】：國家自然科學(xué)青年基金(編號:81400532) 吉林省衛(wèi)生計生青年科研課題(編號:2015q017) 2015年度吉林大學(xué)白求恩培育計劃(編號:2015301) 吉林大學(xué)優(yōu)秀青年教師培養(yǎng)計劃(編號:419080500367)
【分類號】：R782

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