本文選題：頜骨　切入點：牙源性角化囊性瘤　出處：《青島大學》2014年碩士論文

【摘要】：目的： 頜骨牙源性角化囊性瘤(KCOT)發(fā)現(xiàn)時往往己較巨大,可采用微創(chuàng)的開窗灌洗治療,最大限度地保留了頜骨的功能,但治療周期較長,其內(nèi)在信號調(diào)控通路還缺少研究。Wnt信號通路與人類腫瘤的發(fā)生發(fā)展具有密切關系,其在KCOT及其轉(zhuǎn)歸中發(fā)揮何種作用尚需深入研究。本研究應用基因芯片,檢測頜骨KCOT中Wnt信號通路的相關基因,探討Wnt信號通路相關基因在頜骨KCOT中發(fā)揮的作用,并用Wnt信號通路阻斷劑臨床治療KCOT病例,以期縮短臨床療程,并驗證Wnt信號通路相關基因在頜骨KCOT微創(chuàng)功能治療中發(fā)揮的調(diào)控作用。 方法： 1.應用基因芯片,檢測KCOT中的Wnt信號通路的相關基因,探討Wnt信號通路相關基因在頜骨KCOT中發(fā)揮的作用。 2.臨床診斷為頜骨KCOT的病例,分為實驗組與對照組,行全景片與CT檢查,開窗取活檢,明確病理診斷,并行開窗灌洗治療。實驗組應用Wnt信號通路阻斷劑(EGCG)進行囊腔灌洗,對照組應用NS作常規(guī)灌洗。將實驗組和對照組的病例進行比較,觀察阻斷Wnt信號通路相關基因的表達對頜骨KCOT微創(chuàng)功能治療的影響。 結果： 1.基因芯片檢測發(fā)現(xiàn)KCOT中存在Wnt信號通路相關基因的差異表達,其中Wnt分子中的Wnt5a表達上調(diào)；Wnt分子受體FZD3表達上調(diào),Wnt信號傳導通路中的MAPK10, PRKX基因表達上調(diào),CAMK2A表達下調(diào)。 2.實驗組應用Wnt信號通路阻斷劑(EGCG)開窗灌洗治療頜骨KCOT,僅需5至7個月囊腔即消退,對照組應用NS常規(guī)開窗灌洗治療頜骨KCOT,則需1.5年至2年。實驗組應用Wnt信號通路阻斷劑EGCG,使得開窗灌洗治療頜骨KCOT的臨床治療周期大大縮短。 結論： 1.頜骨KCOT中存在Wnt信號通路相關基因的差異表達。 2.Wnt信號通路阻斷劑EGCG開窗灌洗治療頜骨KCOT,大大縮短臨床治療周期。
[Abstract]:Objective:KCOT (Odontogenic keratocystic tumor of jaw) is often found to be very large. It can be treated with minimally invasive fenestration and lavage, and the function of jaw is preserved to the maximum extent, but the treatment period is longer.Wnt signaling pathway is closely related to the occurrence and development of human tumors. The role of Wnt signaling pathway in KCOT and its outcome needs further study.In this study, the gene chip was used to detect the genes related to the Wnt signaling pathway in the jaw KCOT, to explore the role of the Wnt signal pathway related genes in the jaw KCOT, and to use Wnt signal pathway blocker to treat the KCOT cases in order to shorten the clinical course of treatment.The regulatory role of Wnt signaling pathway related genes in KCOT minimally invasive functional therapy of jaw was verified.Methods:1.The gene chip was used to detect the genes related to Wnt signaling pathway in KCOT and to explore the role of Wnt signaling pathway related genes in jaw KCOT.2.The patients diagnosed as KCOT were divided into experimental group and control group. Panoramic film and CT were performed, biopsy was performed, pathological diagnosis was confirmed, and fenestration lavage was performed.The experimental group was treated with Wnt signal pathway blocker (EGCG) and the control group was treated with NS.The effects of blocking Wnt signaling pathway related gene expression on KCOT minimally invasive functional therapy were compared between the experimental group and the control group.Results:1.The differential expression of Wnt signaling pathway related genes was found in KCOT by microarray analysis, in which the expression of Wnt5a in Wnt was up-regulated and the expression of FZD3 was up-regulated in Wnt signaling pathway. The expression of PRKX was up-regulated and the expression of CAMK2A was down-regulated.2.In the experimental group, Wnt signal pathway blocker (EGCG) was used for the treatment of maxillary KCOT. It only took 5 to 7 months for the capsule cavity to dissipate, while for the control group, it took 1.5 years to 2 years to treat the maxillary KCOT with NS routine fenestration lavage.The experimental group was treated with Wnt signal pathway blocker, which shortened the clinical treatment period of KCOT.Conclusion:1.Differential expression of genes related to Wnt signaling pathway exists in jaw KCOT.2.Wnt signal pathway blocker EGCG fenestration in the treatment of jaws KCOT greatly shortened the clinical treatment cycle.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R739.8

【參考文獻】

相關期刊論文 前10條

1 譚曉華,btamail.net.cn,張亞歷,周殿元;EGCG誘導胃癌和肝癌細胞凋亡及bcl-2表達下調(diào)的研究[J];癌癥;2000年07期

2 彭輝;鐘雪云;劉坤平;李素梅;;應用組織芯片檢測食管鱗狀細胞癌中APC、β-catenin、E-cadherin與cyclin D1的表達及其意義[J];癌癥;2009年01期

3 龍建武;梁慶模;;Wnt信號通路與腫瘤及腫瘤靶點治療研究新進展[J];癌癥進展;2009年02期

4 王偉;滿曉華;鄭建明;李兆申;高軍;龔燕芳;;Wnt5a在胰腺癌及癌前病變中的表達差異分析[J];第二軍醫(yī)大學學報;2012年03期

5 江穗;陳錫林;丁詠;陳仲偉;朱李軍;馮航;王啟朋;甄茂川;汪謙;;沒食子兒茶素沒食子酸酯誘導人口腔上皮癌細胞G1期阻滯[J];南方醫(yī)科大學學報;2009年07期

6 蔣自強,趙怡芳;負壓吸引術和減壓術治療牙源性角化囊腫的對比研究[J];華西口腔醫(yī)學雜志;2002年04期

7 胡銳;田超;孟文建;張劍輝;李卉;張蒲容;龍啟明;陶蘋;;Wnt-1誘導分泌蛋白-1在乳腺浸潤性導管癌中的表達及其臨床意義[J];四川大學學報(醫(yī)學版);2010年02期

8 環(huán)奕;;WNT信號通路與腫瘤[J];基礎醫(yī)學與臨床;2006年08期

9 辛俊彤;袁榮濤;卜令學;賈暮云;李寧毅;;開窗減壓術治療頜骨巨大囊性病變的臨床研究[J];口腔醫(yī)學;2011年04期

10 鐘鳴,王巖,岳陽麗,王潔;人成釉細胞瘤中上皮性鈣黏附蛋白和β-連環(huán)素的表達[J];口腔醫(yī)學研究;2003年03期

，

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