本文選題：牙齦卟啉單胞菌　切入點：脂多糖　出處：《細胞與分子免疫學雜志》2017年03期

【摘要】：目的研究牙齦卟啉單胞菌脂多糖(Pg-LPS)對人牙齦成纖維細胞(HGF)自噬功能的影響。方法以10μg/mL Pg-LPS刺激HGF 12 h或24 h,采用雷帕霉素作為陽性對照,Western blot法檢測微管相關(guān)蛋白1輕鏈3B(LC3B)的表達,間接免疫熒光技術(shù)檢測自噬體的分布;同時使用MitoSOX Red熒光探針標記線粒體活性氧(mtROS)、間接免疫熒光法檢測自噬體,觀察Pg-LPS處理后HGF mtROS與線粒體自噬水平;分別以叔丁基-4-羥基茴香醚(BHA)、N-乙酰半胱氨酸(NAC)以及輔酶Q10(CoQ10)阻斷ROS,Western blot法檢測Pg-LPS刺激后LC3B的表達量。結(jié)果 Pg-LPS處理后LC3BⅡ/LC3BⅠ的比值與自噬體數(shù)量均顯著升高,并且24 h處理組高于12 h處理組,同時mtROS生成量與線粒體自噬均明顯增加。此外CoQ10可有效降低Pg-LPS誘導的HGF自噬。結(jié)論 Pg-LPS在HGF中通過觸發(fā)mtROS活化自噬,并且自噬參與受損線粒體的降解以維持細胞內(nèi)環(huán)境穩(wěn)態(tài)。
[Abstract]:Objective to study the effect of Pg-LPSs from porphyromonas gingivalis on the autophagy of human gingival fibroblasts (HGF).Methods 10 渭 g/mL Pg-LPS was used to stimulate HGF for 12 h or 24 h. The expression of microtubule-associated protein 1 light chain 3BC3LC _ 3B was detected by Western blot, and the distribution of autophagy was detected by indirect immunofluorescence technique.At the same time, MitoSOX Red fluorescence probe was used to label mitochondrial reactive oxygen species (Ros) and mtROSs, and indirect immunofluorescence assay was used to detect autophagy. The levels of HGF mtROS and mitochondrial autophagy after Pg-LPS treatment were observed.The expression of LC3B after Pg-LPS stimulation was detected by blot method with blot and coenzyme Q10 CoQ10 with tert-#china_person0#-4-hydroxyanisole (BHA) and coenzyme Q10-CoQ10 (n-acetylcysteine).Results after Pg-LPS treatment, the ratio of LC3B 鈪，

本文編號：1702851