本文選題：口腔感染　切入點(diǎn)：假絲酵母菌　出處：《中國病原生物學(xué)雜志》2017年11期

【摘要】：目的研究口腔感染假絲酵母菌構(gòu)成以及耐藥情況。方法選取2016年1月-2017年6月本院診治口腔感染假絲酵母菌患者462例,收集口腔患處樣本并培養(yǎng)。采用顯色培養(yǎng)基和VITEK全自動微生物分析儀鑒定菌株。采用K-B紙片法檢測假絲酵母菌對臨床常用的5種抗生素耐藥情況,判讀標(biāo)準(zhǔn)依據(jù)CLSI 2017。將CAP1-mRNA基因進(jìn)行熒光定量擴(kuò)增,并比較白色假絲酵母菌耐藥株和敏感株的CAP1-mRNA表達(dá)水平。對耐藥株ERG11基因PCR擴(kuò)增,陽性產(chǎn)物雙向測序。結(jié)果共培養(yǎng)出假絲酵母菌屬173株,其中白色假絲酵母菌、光滑假絲酵母菌、熱帶假絲酵母菌、近平滑假絲酵母菌和克柔假絲酵母菌分別為105、38、17、9和4株,分別占60.69%、21.97%、9.83%、5.20%和2.31%。12株白色假絲酵母菌對除兩性霉素外其余四種臨床常用抗真菌藥物均產(chǎn)生不同程度耐藥,光滑假絲酵母菌僅2株對氟康唑產(chǎn)生耐藥,其他假絲酵母菌未產(chǎn)生耐藥。12株白色假絲酵母菌中6株是多重耐藥菌,2株對四種抗生素同時產(chǎn)生耐藥性。12株白色假絲酵母菌耐藥株CAP1-mRNA表達(dá)水平為9.5±2.21,敏感株表達(dá)水平為7.2±1.87,差異有統(tǒng)計學(xué)意義(t=2.45,P0.05);12株菌株ERG11基因不同程度發(fā)生位點(diǎn)突變,共計發(fā)生同義突變11處,錯義突變14處。結(jié)論白色假絲酵母菌是口腔假絲酵母菌感染中的優(yōu)勢菌種。白色假絲酵母菌耐藥與CAP1-mRNA表達(dá)水平和ERG11基因位點(diǎn)突變關(guān)系密切。
[Abstract]:Objective to study the composition and drug resistance of Candida cerevisiae in oral infection. Methods 462 patients with Candida cerevisiae from January 2016 to June 2017 were selected. The strains were identified by color medium and VITEK automatic microbiological analyzer. K-B disk method was used to detect the resistance of Candida cerevisiae to 5 kinds of antibiotics commonly used in clinic. The standard of interpretation was based on CLSI 2017.The CAP1-mRNA gene was amplified by fluorescence quantitative amplification, and the CAP1-mRNA expression levels of resistant and sensitive strains of Candida albicans were compared. PCR amplification of ERG11 gene was performed on resistant strains. Results 173 strains of Candida, including Candida albicans, Candida smoothing, Candida tropicalis, Candida tropicalis, Candida tropicalis, Candida tropicalis and Candida cerevisiae, were isolated from 173 strains of Candida. The resistance of 12 strains of Candida albicans to four common antifungal drugs except amphotericin was found to be different degree, and only 2 strains of Candida smooth were resistant to fluconazole. Other Candida albicans did not produce Drug Resistance. 6 of 12 Candida albicans strains were multidrug resistant. 2 strains were resistant to four antibiotics. 12 Candida albicans resistant strains showed CAP1-mRNA expression level of 9.5 鹵2.21, and sensitive strains expressed water. The level was 7.2 鹵1.87, the difference was statistically significant (P 0.05). The ERG11 gene mutation occurred in different degree in 12 strains. There were 11 synonymous mutations and 14 missense mutations. Conclusion Candida albicans is the dominant strain in oral Candida. Drug resistance of Candida albicans is closely related to CAP1-mRNA expression level and ERG11 gene mutation.
【作者單位】： 鄭州大學(xué)附屬鄭州中心醫(yī)院;北京中醫(yī)藥大學(xué)東方醫(yī)院;開封衛(wèi)校附屬醫(yī)院;濮陽市第三人民醫(yī)院;
【分類號】：R446.5;R781.5

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