本文選題：牙髓干細胞　切入點：同種異體　出處：《新疆醫(yī)科大學》2017年碩士論文

【摘要】：目的:初步探究小鼠牙髓干細胞同種異體移植后的全身免疫反應。方法:牙髓干細胞分離獲得自C57BL/6小鼠牙髓組織,培養(yǎng)至第3代,與納米羥基磷灰石(n-HA)復合培養(yǎng)形成復合物,掃描電鏡觀察其生長黏附情況。40只4周齡的C57BL/6小鼠隨機分成兩組。實驗組將復合物移植入上背部皮下,對照組將納米羥基磷灰石植入上背部皮下,術后均使用外科方法嚴密縫合創(chuàng)口。兩組動物術后均觀察4周、6周處死,分離脾臟,制成脾淋巴細胞懸液,流式細胞儀測定CD4~+、CD8~+的值。結果:牙髓干細胞在體外與納米羥基磷灰石結合良好;免疫組化染色實驗組4周、6周均可見牙本質涎蛋白(dentin sialoprotein,DSP)陽性表達;實驗組與對照組相比,術后4周、6周CD4~+/CD8~+值均無明顯差異(P0.05)。結論:牙髓干細胞同種異體移植后,尚不能夠引起機體的免疫排斥反應,其在體內仍具有低免疫原性,有望成為組織工程同種異體種子細胞來源。
[Abstract]:Objective: to investigate the systemic immune response of mouse dental pulp stem cells after allograft transplantation. Methods: dental pulp stem cells were isolated from C57BL/6 mouse dental pulp tissue, cultured to the third generation, and co-cultured with nano-hydroxyapatite (HA) to form complex. 40 C57BL/6 mice aged 4 weeks were randomly divided into two groups: the experimental group transplanted the composite into the upper back subcutaneously, the control group implanted nano-hydroxyapatite into the upper back subcutaneously, and the control group implanted nano-hydroxyapatite into the upper back subcutaneously. The wounds were sutured closely after operation. The animals in both groups were killed at 4 weeks and 6 weeks after operation, and the spleen was separated and made into splenic lymphocyte suspensions. Results: dental pulp stem cells combined well with nano-hydroxyapatite in vitro, the positive expression of dentin sialoprotein DSPs was observed in experimental group after 4 weeks and 6 weeks of immunohistochemical staining. There was no significant difference in CD4 ~ / CD8 ~ value between 4 and 6 weeks postoperatively. Conclusion: after dental pulp stem cell allograft transplantation, it can not cause immune rejection, and it still has low immunogenicity in vivo. It is expected to be a source of allogeneic seed cells for tissue engineering.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R781

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