本文選題：金屬蛋白酶解離素　切入點：反義核酸　出處：《上�？谇会t(yī)學》2014年05期

【摘要】：目的:研究金屬蛋白酶解離素28(a disintegrin and metalloproteinase 28,ADAM28)反義核酸(antisense oligodeoxynucleotide,AS-ODN)對人牙齦成纖維細胞(human gingival fibroblasts,HGFs)增殖、分化、凋亡的影響,并分析可能的作用機制。方法:采用細胞培養(yǎng)、基因轉(zhuǎn)染、四甲基唑藍(MTT)比色法、酶動力學法和流式細胞術(shù)(FCM)檢測ADAM28反義核酸轉(zhuǎn)染HGFs后對細胞生物學特性的影響。采用SPSS16.0軟件包中的SNK檢驗對數(shù)據(jù)進行統(tǒng)計學分析。結(jié)果:ADAM28 AS-ODN組HGFs的增殖活性顯著降低。AS-ODN組HGFs處于S期的細胞百分比顯著低于SODN組和未轉(zhuǎn)染組,G2+M期的細胞百分比顯著低于未轉(zhuǎn)染組,AS-ODN組的細胞增殖指數(shù)(PI=S+G2M)顯著低于其他2組,差異顯著。AS-ODN組堿性磷酸酶(ALP)分泌活性、凋亡細胞百分比顯著升高。結(jié)論:ADAM28反義核酸可顯著抑制HGFs的增殖,并影響細胞周期的變化,促進其分化,顯著誘導(dǎo)HGFs的凋亡。
[Abstract]:Objective: To study the disintegrin metalloproteinase 28 (a disintegrin and metalloproteinase 28, ADAM28) antisense RNA (antisense oligodeoxynucleotide, AS-ODN) on human gingival fibroblasts (human gingival, fibroblasts, HGFs) proliferation, differentiation, apoptosis, and the possible mechanism. Methods: cell culture, gene transfection, four methyl tetrazolium (MTT) colorimetric assay, enzyme kinetics assay and flow cytometry (FCM) effect on the biological characteristics of cells detected antisense ADAM28 transfected HGFs. SNK test was used in the SPSS16.0 software package for statistical analysis of data. Results: ADAM28 AS-ODN group HGFs.AS-ODN group HGFs significantly decreased the proliferation activity in the percentage of cells in S phase was significantly lower than that in SODN group and non transfection group, the percentage of cells in G2+M phase was significantly lower than the non transfection group, AS-ODN group, the cell proliferation index (PI=S+G2M) was significantly lower than that of the other 2 groups, Significant difference was observed in alkaline phosphatase (ALP) secreting activity and percentage of apoptotic cells in group.AS-ODN. Conclusion: ADAM28 antisense nucleic acid can significantly inhibit HGFs proliferation, influence cell cycle change, promote differentiation and induce HGFs apoptosis.

【作者單位】： 解放軍第401醫(yī)院口腔科;
【基金】：中國博士后科學基金特別資助課題(201003774)~~
【分類號】：R781

【參考文獻】

相關(guān)期刊論文 前4條

1 趙征;劉洪臣;黃征難;;ADAM28反義核酸對人牙周膜干細胞增殖、分化和凋亡的影響[J];上�？谇会t(yī)學;2009年05期

2 王琨;許彥枝;朱f趂，

本文編號：1655590