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  本文選題：新型3F-PAc樹脂基質(zhì)　切入點：Bis-GMA　出處：《吉林大學(xué)》2017年碩士論文

【摘要】：簡介:復(fù)合樹脂類牙科修復(fù)用材料因其操作簡單快捷,色澤美觀性佳,與天然牙相似度高而越來越被普遍應(yīng)用于口腔科的修復(fù)治療中,然而盡管隨著材料工藝的不斷發(fā)展,復(fù)合樹脂類材料有了很大的進(jìn)步,但仍舊存在著三大缺陷,即聚合收縮、耐磨性差及生物相容性的問題。近年來,隨著生活水平的提高,口腔材料的生物安全性問題逐漸引起人們的重視,而樹脂材料的聚合收縮率與材料的生物安全性具有一定的相關(guān)性,基于以上原因推動了國內(nèi)外學(xué)者致力于研發(fā)出新型的機(jī)械性能更好,安全性更佳的口腔修復(fù)用牙科樹脂材料。每一種新研發(fā)的口腔材料更需經(jīng)過生物安全性檢測,細(xì)胞毒性檢測是其中一項重要的參考內(nèi)容,本研究即針對一種新研發(fā)的具有較低聚合收縮性能的樹脂材料的細(xì)胞毒性性進(jìn)行檢測,為該復(fù)合樹脂材料在臨床應(yīng)用提供生物安全性的依據(jù),同時也對樹脂材料中的一種重要稀釋劑TEGDMA成分對樹脂材料細(xì)胞毒性的影響做了初步研究。目的:評價一種新型低聚合收縮樹脂材料的浸提液對L929小鼠成纖維細(xì)胞的細(xì)胞毒性,以及不同濃度梯度的材料浸提液對細(xì)胞增殖的影響,進(jìn)而判斷其毒性的大小,并初步分析復(fù)合樹脂基質(zhì)中的稀釋劑TEGDMA對材料毒性的影響,為后續(xù)研究奠定基礎(chǔ)。方法:由本課題組于實驗室合成新型低聚合收縮樹脂基質(zhì)——間三氟甲基苯基型丙烯酸酯3F-PAc,與臨床上傳統(tǒng)常用樹脂基質(zhì)Bis-GMA作比較,將實驗組分為三組,分別為Bis-GMA組、3F-PAc 1組和3F-PAc 0組,3F-PAc 0組不添加稀釋劑TEGDMA。制備三組材料浸提液,連續(xù)稀釋為4種濃度100%、50%、25%、12.5%;采用甲基噻唑基四唑比色法(methyl thiazolyl tetrazolium,MTT)檢測小鼠成纖維細(xì)胞L929與各稀釋液共培養(yǎng)1、3、5d后的細(xì)胞相對增值率并與陰性對照組作比較,評價細(xì)胞毒性等級。陰性對照組只加含10%胎牛血清的DMEM完全培養(yǎng)基,陽性對照組為0.64%苯酚的浸提液。結(jié)果與結(jié)論:三組材料的浸漬液均表現(xiàn)出了程度不等的細(xì)胞毒性作用,除培養(yǎng)3d和5d時Bis-GMA和3F-PAc 1組的100%浸液毒性等級為2級外,其余各實驗組浸提液細(xì)胞毒性的等級在0~1級之間。3F-PAc 0組浸提液吸光度值大于3F-PAc1組與Bis-GMA組,其差異具有統(tǒng)計學(xué)意義(P0.05),3F-PAc 1組與Bis-GMA組之間差異不顯著(P0.05)。綜上所述得出結(jié)論:三種復(fù)合樹脂材料的細(xì)胞毒性的評級都在安全的范圍內(nèi),新型3F-PAc樹脂基質(zhì)材料細(xì)胞毒性等級符合標(biāo)準(zhǔn),具有較好的生物安全性;稀釋劑TEGDMA可能對樹脂材料的細(xì)胞毒性有一定影響。
[Abstract]:Summary: composite resin dental restoration materials are more and more widely used in stomatology because of their simple and quick operation, beautiful color and high similarity with natural teeth. However, with the development of material technology, Composite resin materials have made great progress, but there are still three major defects, namely, polymerization shrinkage, poor wear resistance and biocompatibility. In recent years, with the improvement of living standards, People pay more and more attention to the biosafety of oral materials, and the polymerization shrinkage rate of resin materials has a certain correlation with the biosafety of materials. For these reasons, scholars at home and abroad are committed to developing new dental resin materials with better mechanical properties and better safety. Cytotoxicity detection is one of the important reference contents. In this study, the cytotoxicity of a new resin material with low polymerization shrinkage was studied. It provides the basis of biosafety for the clinical application of the composite resin material. At the same time, the effects of an important diluent TEGDMA on the cytotoxicity of resin materials were studied. Objective: to evaluate the cytotoxicity of a new type of low polymerization shrinkage resin to L929 mouse fibroblasts. The effects of different concentration gradient material extract on cell proliferation and the toxicity of TEGDMA in composite resin matrix were also analyzed, and the effects of the diluent TEGDMA in the composite resin matrix on the toxicity of the composite resin matrix were preliminarily analyzed, and the effects of the diluent TEGDMA on the toxicity of the composite resin matrix were analyzed. Methods: a new type of low polymerization shrinkage resin matrix, m-trifluoromethylphenyl acrylate (3F-PAc), was synthesized in our laboratory. The experimental group was divided into three groups, compared with the traditional resin matrix Bis-GMA. Three groups of material extracts were prepared from Bis-GMA group and 3F-PAc group 0 group without adding diluent, respectively. The relative proliferation rate of mouse fibroblasts L929 was detected by methyl thiazolyl tetrazolium MTT, and the cell proliferation rate was compared with that of the negative control group after co-cultured with each diluent for 5 days. To evaluate the cytotoxicity grade, the negative control group was only added with 10% fetal bovine serum DMEM complete medium, and the positive control group was 0.64% phenol extract. Results and conclusion: the three groups showed different cytotoxic effects. Except that the toxicity grade of 100% extract of Bis-GMA and 3F-PAc 1 group was grade 2 at 3 and 5 days after culture, the cell toxicity of the other experimental groups was higher than that of 3F-PAc1 group and Bis-GMA group in the range of 0 ~ (-1). 3 F-PAc _ 0 group was higher than that of 3F-PAc1 group and Bis-GMA group. The difference was statistically significant. There was no significant difference between Bis-GMA group and P0.05 group. In summary, it was concluded that the cytotoxicity ratings of the three composite resin materials were all within the safe range. The cytotoxic grade of the new 3F-PAc resin matrix material is up to the standard and has good biological safety, and the diluent TEGDMA may have some influence on the cytotoxicity of the resin material.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R783.1

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