本文選題：Twisted　切入點(diǎn)：Gastrulation　出處：《南昌大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：研究背景： 骨纖維異常增殖癥又稱骨纖維結(jié)構(gòu)不良，是一種先天非遺傳性骨組織疾病，男女均可患病，通常在兒童時(shí)期起病，青少年后才出現(xiàn)癥狀，病程較長(zhǎng)，好發(fā)于頜面部，，頜面部FD臨床表現(xiàn)為慢性無(wú)痛性腫脹隆起，進(jìn)一步生長(zhǎng)時(shí)引起面部左右不對(duì)稱，侵犯臨近組織時(shí)可表現(xiàn)如鼻塞、膿涕，咬合錯(cuò)位，眼移位伴復(fù)視，聽(tīng)力下降，頭痛等癥狀。多數(shù)患者需要通過(guò)手術(shù)切除病損，往往造成外觀畸形和功能障礙，給患者帶來(lái)極大痛苦，并嚴(yán)重影響患者的生活質(zhì)量，且局部切除術(shù)后復(fù)發(fā)概率高，給患者造成了極大的痛苦。研究與骨纖維異常增殖癥發(fā)生和發(fā)展的相關(guān)因子，為骨纖維異常增殖癥的非手術(shù)治療尋找新的方法，降低治療后的復(fù)發(fā)率具有重要的意義。 目的: 通過(guò)檢測(cè)twisted gastrulation(TWSG1)蛋白在顱頜面骨纖維異常增殖癥中的表達(dá)情況，分析其表達(dá)與顱頜面骨纖維異常增殖癥患者的表達(dá)水平與年齡因素及預(yù)后關(guān)系，旨在研究TWSG1的表達(dá)在顱頜面骨纖維異常增殖癥中的作用，可以進(jìn)一步加強(qiáng)對(duì)TWSG1基因的認(rèn)識(shí)，且可利用TWSG1進(jìn)行顱頜面骨纖維異常增殖癥的分子及基因治療提供相關(guān)理論依據(jù)。 方法： 取44例顱頜面骨纖維異常增殖癥石蠟標(biāo)本及10例頜骨骨折外傷骨碎片標(biāo)本，采用免疫組織化學(xué)Envision法檢測(cè)TWSG1蛋白在頜面骨纖維異常增殖癥和正常頜骨組織中的表達(dá)水平，將檢測(cè)結(jié)果與患者的臨床特征進(jìn)行相關(guān)性分析。所有資料均運(yùn)用SPSS19.0進(jìn)行統(tǒng)計(jì)學(xué)分析，采用秩和檢驗(yàn)，P0.05為差異有顯著性意義。 結(jié)果： 1、顱頜面部骨纖維異常增殖癥中TWSG1的陽(yáng)性表達(dá)（44/44）在細(xì)胞質(zhì)/膜內(nèi)呈棕黃色顆粒如圖所示，主要見(jiàn)于骨母細(xì)胞及成纖維細(xì)胞。而正常頜骨組織中TWSG1的表達(dá)陽(yáng)性（2/10）。 2、FD組織TWSG1表達(dá)與年齡及預(yù)后關(guān)系FD組織TWSG1表達(dá)與年齡及預(yù)后關(guān)系見(jiàn)表1。FD患者TWSG1皆陽(yáng)性表達(dá)，明顯高于正常頜骨陰性對(duì)照組（P＜0.05）；未成年FD患者TWSG1表達(dá)也明顯高于成年組（P＜0.05）；以預(yù)后復(fù)發(fā)與否分類，亦可見(jiàn)預(yù)后良好的FD患者TWSG1表達(dá)明顯低于預(yù)后差的患者（P＜0.05）。 結(jié)論： 1、TWSG1蛋白在顱頜骨骨纖維異常增殖癥組織中表達(dá)上升并高表達(dá)，TWSG1可能促進(jìn)了頜面部骨化性纖維瘤的發(fā)生與發(fā)展，并可能在其中發(fā)揮了重要作用。 2、FD的TWSG1的陽(yáng)性表達(dá)水平與疾病發(fā)展進(jìn)程密切相關(guān)，是FD患者預(yù)后不良的重要生物學(xué)指標(biāo)。
[Abstract]:Background:. Dysplasia of bone fiber, also known as dysplasia of bone fiber, is a congenital non-hereditary bone tissue disease. Both men and women are ill. It usually starts in childhood, and symptoms appear only after adolescence. The course of disease is relatively long, and it is prone to occur in the maxillofacial region. The clinical manifestations of FD in maxillofacial region were chronic painless swelling and protuberance, which caused asymmetry of left and right face during further growth, and may be shown as nasal obstruction, purulent snot, malocclusion, eye displacement with diplopia and hearing loss when invading adjacent tissues. Headache and other symptoms. Most patients need surgery to remove the lesions, often resulting in appearance deformities and dysfunction, bring great pain to patients, and seriously affect the quality of life of patients, and local resection after the recurrence rate is high, It is of great significance to study the factors related to the occurrence and development of osteofibrillary dysplasia, to find a new method for non-operative treatment of osteofibrillary dysplasia, and to reduce the recurrence rate after treatment. Objective:. The expression of twisted gastrulation TWSG1 protein in craniomaxillofacial fibrous dysplasia was detected, and the relationship between the expression level and age factors and prognosis of patients with craniomaxillofacial fibrous dysplasia was analyzed. The purpose of this study was to study the role of TWSG1 expression in craniomaxillofacial fibrous dysplasia, and to further enhance the understanding of TWSG1 gene, and to use TWSG1 for molecular and gene therapy of craniomaxillofacial fibrous dysplasia. Methods:. Paraffin wax specimens from 44 cases of craniomaxillofacial fibrous dysplasia and 10 cases of traumatic bone fragments from maxillofacial fractures were used to detect the expression of TWSG1 protein in maxillofacial fibrous dysplasia and normal maxillofacial tissues by immunohistochemical Envision method. All the data were statistically analyzed by SPSS19.0, and the difference was significant by rank sum test (P0.05). Results:. 1. The positive expression of TWSG1 in craniomaxillofacial fibrous dysplasia (44 / 44) was brown in cytoplasm / membrane, mainly in osteoblasts and fibroblasts, while in normal maxillofacial tissues, the positive expression of TWSG1 was 2 / 10 / 10. 2the expression of TWSG1 and age and prognosis in FD tissue; the expression of TWSG1 in FD tissue and the relationship between age and prognosis were all positive in 1.FD patients. The expression of TWSG1 in the juvenile patients with FD was significantly higher than that in the adult group (P < 0.05), and the expression of TWSG1 in the patients with good prognosis was significantly lower than that in the patients with poor prognosis (P < 0.05). Conclusion:. 1the expression of TWSG1 protein in craniomaxillary fibrous dysplasia tissues increased and the high expression of TWSG1 may promote the occurrence and development of ossifying fibroma of maxillofacial region, and may play an important role in it. 2the positive expression level of TWSG1 in FD is closely related to the progression of the disease and is an important biological indicator of poor prognosis in FD patients.
【學(xué)位授予單位】：南昌大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R782

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相關(guān)期刊論文 前1條

1 李恩超;BMP_2、IL-6在骨的纖維結(jié)構(gòu)不良中的表達(dá)及臨床意義[J];中國(guó)骨腫瘤骨病;2005年03期

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