本文選題：葉酸　切入點：殼聚糖　出處：《廣西醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:合成性質(zhì)穩(wěn)定的葉酸殼聚糖載地西他濱納米粒,觀察其對體外培養(yǎng)人鱗狀上皮舌癌SCC-9細(xì)胞的抑制率,探討納米粒對腫瘤細(xì)胞的靶向作用機(jī)制。方法:采用離子交聯(lián)法合成葉酸殼聚糖載地西他濱納米粒,透射電鏡觀察其形態(tài),納米粒度分析儀測量納米粒平均粒徑、電位,檢測載藥率以及包封率。將體外培養(yǎng)的處于對數(shù)期的人鱗狀上皮舌癌SCC-9細(xì)胞分為0、1、2、3、4、5、6、7八組。1、2、3組選用三個梯度濃度1×l0-7mol/L、1×l0-6mol/L、1×l0-5mol/L的DAC處理細(xì)胞,4、5、6組采用同樣三個濃度梯度1×l0-7mol/L、1×l0-6 mol/L、1×l0-5mol/L的葉酸殼聚糖載地西他濱納米粒處理細(xì)胞,7組為葉酸殼聚糖空載體組,0組為空白對照組。MTT法檢測各組對舌癌細(xì)胞的抑制率。結(jié)果:葉酸殼聚糖載地西他濱納米粒形態(tài)為球形,分布均勻,平均粒徑為212nm,Zeta電位絕對值平均值為31.8mv,性質(zhì)穩(wěn)定。載藥納米粒的包封率為75%,載藥率為18%。葉酸殼聚糖載地西他濱納米粒組較單純地西他濱組對人鱗狀上皮舌癌SCC-9細(xì)胞抑制率高,且差異具有統(tǒng)計學(xué)意義。結(jié)論:(1)本實驗成功合成葉酸殼聚糖載地西他濱納米粒;(2)葉酸殼聚糖載地西他濱納米粒性質(zhì)穩(wěn)定,載藥率以及包封率較高;(3)葉酸殼聚糖載地西他濱納米粒可能靶向作用于人鱗狀上皮舌癌SCC-9細(xì)胞,抑制SCC-9的生長。
[Abstract]:Aim: to synthesize stable chitosan-loaded dicitabine nanoparticles and observe its inhibitory rate on human squamous cell carcinoma of tongue (SCC-9) cells in vitro. Methods: chitosan folate loaded dicitabine nanoparticles were synthesized by ion crosslinking method. The morphology of nanoparticles was observed by transmission electron microscope. The average particle size and potential of nanoparticles were measured by nano-particle size analyzer. The SCC-9 cells in logarithmic phase of human squamous cell carcinoma of tongue were divided into three groups: 1 脳 l0-7 mol / L 1 脳 l0-6 mol / L 1 脳 l0-6 mol / L 1 脳 l0-6 mol / L DAC with the same concentration gradient 1 脳 l0-7 mol / L 1 脳 l0-6 mol / L ~ (-1) 脳 l0-5 mol / L ~ (-1) 脳 l0-5 mol / L ~ (-1). The inhibitory rate of folate chitosan loaded dietabine nanoparticles on tongue cancer cells was detected by MTT method. Results: the morphology of the nanoparticles was spherical. Evenly distributed, The mean particle size was 212nmPe, the mean absolute value of Zeta potential was 31.8mv.The entrapment efficiency of drug loaded nanoparticles was 75 and the drug loading rate was 18.The inhibitory rate of dietabine nanoparticles loaded with folate chitosan on SCC-9 cells of human squamous epithelial tongue carcinoma was higher than that of dietabine group. ConclusionChitosan-loaded dietabine nanoparticles containing folate chitosan were successfully synthesized in this experiment. The drug loading rate and encapsulation efficiency were higher than that of the control group) chitosan / dicitabine nanoparticles might target SCC-9 cells and inhibit the growth of SCC-9 in human squamous cell carcinoma of tongue.
【學(xué)位授予單位】：廣西醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R739.86

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