本文選題：骨保護(hù)素　切入點：聚乳酸羥基乙酸　出處：《安徽醫(yī)科大學(xué)學(xué)報》2017年11期 　論文類型：期刊論文

【摘要】：目的以聚乳酸羥基乙酸(PLGA)為載體構(gòu)建載有骨保護(hù)素(OPG)的微球,篩選出緩釋效果最佳的制備條件,并研究載藥微球的體外釋放特性。方法采用復(fù)乳溶劑揮發(fā)法,以不同的攪拌速度、聚乙烯醇(PVA)濃度、PLGA濃度制備OPG-PLGA微球并測定其載藥量和包封率,通過正交試驗優(yōu)化制備條件;以磷酸鹽緩沖液作為釋放介質(zhì)考察載藥微球的體外釋放特性。結(jié)果以PLGA聚合物濃度400 mg/ml、攪拌速度400 r/min、PVA濃度2%為條件制備的載藥微球具有最優(yōu)的載藥量和包封率,分別為6.21×10-7和75.10%,體外釋藥試驗顯示微球持續(xù)釋放時間達(dá)到30 d,具有良好緩釋效果。結(jié)論采用優(yōu)化條件制備的OPG-PLGA微球具有較高的包封率和載藥量,同時具有良好的緩釋效果,為用于拔牙位點保存術(shù)的緩釋藥物研究提供了基礎(chǔ)。
[Abstract]:Objective to construct the microspheres containing osteoprotegerin (OPG) with polylactic acid glycolate (PLGA) as the carrier, to select the best preparation conditions for the sustained release, and to study the release characteristics of the drug loaded microspheres in vitro. Methods the compound emulsion solvent volatilization method was used. OPG-PLGA microspheres were prepared with different stirring speed and PVA concentration and their drug loading and encapsulation efficiency were determined. The preparation conditions were optimized by orthogonal test. The release characteristics of drug loaded microspheres were investigated by using phosphate buffer solution as release medium. Results the drug loaded microspheres prepared with the concentration of PLGA polymer 400 mg / ml and stirring speed of 400 r / min PVA concentration 2% had the best drug loading and encapsulation efficiency. The drug release test in vitro showed that the sustained release time of the microspheres was 30 days, and the sustained release effect was good. Conclusion the OPG-PLGA microspheres prepared under the optimized conditions have higher encapsulation efficiency, higher drug loading and better sustained release effect. It provides a basis for the study of sustained-release drugs for tooth extraction site preservation.
【作者單位】： 鄭州大學(xué)第一附屬醫(yī)院口腔種植科;四川大學(xué)華西口腔醫(yī)院粘膜科;
【基金】：河南省醫(yī)學(xué)科技攻關(guān)計劃項目(編號:201303096)
【分類號】：R782.1

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