本文選題：樁核冠　切入點(diǎn)：變異鏈球菌　出處：《華西口腔醫(yī)學(xué)雜志》2014年01期 　論文類型：期刊論文

【摘要】：目的研究老年人樁核冠修復(fù)前后不同時(shí)期根面菌斑中主要致齲菌定植數(shù)量變化。方法以單純隨機(jī)法選擇下頜第一磨牙無咬合功能的患者30人,將一側(cè)需行樁核冠修復(fù)的第一磨牙和對(duì)側(cè)健康第一磨牙分為受試牙和對(duì)照牙。受試牙預(yù)備前、預(yù)備后72 h、預(yù)備后1周及冠修復(fù)后1個(gè)月,分別采集受試牙與對(duì)照牙根面菌斑,然后進(jìn)行厭氧培養(yǎng)、分離,通過菌落形態(tài)學(xué)檢查、生化特征及聚合酶鏈反應(yīng)(PCR)對(duì)其中變異鏈球菌、內(nèi)氏放線菌和黏性放線菌進(jìn)行鑒定和菌落計(jì)數(shù)。結(jié)果受試牙變異鏈球菌預(yù)備前、預(yù)備后72 h、預(yù)備后1周、修復(fù)后1個(gè)月菌落計(jì)數(shù)的變化差異有統(tǒng)計(jì)學(xué)意義(P0.05)。受試牙黏性放線菌菌落計(jì)數(shù)在預(yù)備前、預(yù)備后72 h、預(yù)備后1周、修復(fù)后1個(gè)月的變化差異有統(tǒng)計(jì)學(xué)意義(P0.05)。受試牙內(nèi)氏放線菌菌落計(jì)數(shù)在預(yù)備前、預(yù)備后72 h及1周、修復(fù)后1月的變化差異有統(tǒng)計(jì)學(xué)意義(P0.05)。結(jié)論變異鏈球菌、內(nèi)氏放線菌及黏性放線菌在預(yù)備后1周菌落計(jì)數(shù)增加,修復(fù)后1個(gè)月菌落計(jì)數(shù)降低。以上結(jié)果提示在牙體預(yù)備后初期,應(yīng)指導(dǎo)患者進(jìn)行菌斑控制。
[Abstract]:Objective to study the changes of the number of major cariogenic bacteria in root plaque before and after post core crown restoration in the elderly. Methods Thirty patients with no occlusal function of mandibular first molar were selected by random method. The first molar and the contralateral healthy first molar were divided into two groups. Before preparation, 72 hours after preparation, 1 week after preparation and 1 month after crown restoration, the first molar and the control dental plaque were collected respectively. Then anaerobic culture, isolation, colony morphology, biochemical characteristics and polymerase chain reaction (PCR) were used to detect Streptococcus mutans. Results before preparation, 72 hours after preparation, 1 week after preparation, actinomycetes and actinomycetes were identified and counted. The change of colony count in 1 month after restoration was statistically significant (P 0.05). The count of actinomycetes in teeth was before preparation, 72 hours after preparation, and 1 week after preparation. The change of actinomycetes in teeth was significantly different at 1 month after repair (P 0.05). The count of actinomycetes in teeth was significantly different before preparation, 72 h and 1 week after preparation, and on January after repair. The colony count of actinomycetes Nei and actinomycetes viscosity increased at 1 week after preparation and decreased at 1 month after repair. These results suggest that plaque control should be guided in the early stage of dental preparation.
【作者單位】： 解放軍總醫(yī)院口腔醫(yī)學(xué)研究所;口腔疾病研究國(guó)家重點(diǎn)實(shí)驗(yàn)室華西口腔醫(yī)院(四川大學(xué));
【基金】：四川省科技計(jì)劃基金資助項(xiàng)目(2013SZ0008) 解放軍總醫(yī)院臨床扶持基金資助項(xiàng)目(2012FC-TSYS-2003)
【分類號(hào)】：R783.2

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