本文關(guān)鍵詞： 等離子體 丙烯酸樹脂 白色念珠菌 生物膜 消毒 根管銼 表面殘留物 氬氣等離子體 清理　出處：《南京醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：第一部分等離子體對(duì)樹脂基托表面白色念珠菌的殺滅研究實(shí)驗(yàn)一等離子體對(duì)樹脂基托表面浮游白色念珠菌的殺滅試驗(yàn)?zāi)康?比較氬氣、30%H_2O_2和50%H_2O_2等離子體對(duì)樹脂基托表面浮游白色念珠菌的殺滅效果。方法:用聚甲基丙烯酸甲酯材料制作成9mm×9mm×2mm的試件56塊,在濃度為1.0×108CFU/ml白色念珠菌PBS菌懸液中培養(yǎng)2h后取出,PBS輕漂兩次,分為14組,每組4塊試件。對(duì)照組不做處理,氬氣等離子體分別處理20s、40s、60s、80s、100s,30%H_2O_2和50%H_2O_2等離子體分別處理5s、10s、15s、20s。處理后PBS液洗脫、系列稀釋、涂板。48h后計(jì)數(shù)菌落形成單位,計(jì)算殺滅對(duì)數(shù)值。參考?xì)W洲標(biāo)準(zhǔn)EN 1275-2005,處理后真菌減少4 log10CFU視為有效。結(jié)果:氬氣等離子體處理100s、30%H_2O_2和50%H_2O_2等離子體處理15s后,基托表面浮游白色念珠菌數(shù)量降低均4 log10CFU,均能達(dá)到有效殺滅。結(jié)論:氬氣、30%H_2O_2和50%H_2O_2等離子體均能有效殺滅樹脂基托表面浮游白色念珠菌。30%H_2O_2和50%H_2O_2等離子體的殺菌性能優(yōu)于氬氣等離子體。實(shí)驗(yàn)二等離子體對(duì)樹脂基托表面白色念珠菌生物膜的殺滅試驗(yàn)?zāi)康?評(píng)價(jià)氬氣、30%H_2O_2和50%H_2O_2等離子體對(duì)樹脂基托表面生物膜內(nèi)白色念珠菌的殺滅效果。方法:將聚甲基丙烯酸甲酯材料制作成大小為9mm×9mm×2mm試件68塊,在試件表面培養(yǎng)48h白色念珠菌生物膜。取64塊試件隨機(jī)分為對(duì)照組和15個(gè)處理組(n=4),對(duì)照組不做處理,氬氣等離子體分別處理 60s、120s、180s、240s、300s,30%H_2O_2 和 50%H_2O_2 等離子體分別處理20s、40s、60s、80s、100s。處理后超聲清洗、系列稀釋、涂板。48h后計(jì)數(shù)菌落形成單位,計(jì)算殺滅對(duì)數(shù)值,評(píng)估殺菌效果。參考?xì)W洲標(biāo)準(zhǔn)EN 1275-2005,處理后真菌減少4 log10CFU視為有效。剩余4塊生物膜試件:1塊作為對(duì)照組,其余3塊分別用氬氣、30%H_2O_2和50%H_2O_2等離子體處理300s,通過掃描電鏡觀察生物膜內(nèi)白色念珠菌的損傷。結(jié)果:氬氣等離子體處理300s、30%H_2O_2等離子體處理100s、50%H_2O_2等離子體處理80s后,基托表面生物膜內(nèi)白色念珠菌數(shù)量減少均4 log10CFU。電鏡下等離子體處理后的細(xì)胞膜表面粗糙,有孔洞,完整性破壞。結(jié)論:氬氣、30%H_2O_2和50%H_2O_2等離子體均能有效殺滅生物膜內(nèi)的白色念珠菌。30%H_2O_2和50%H_2O_2等離子體對(duì)白色念珠菌生物膜的殺滅效果較氬氣等離子體更強(qiáng)。第二部分氬氣等離子體對(duì)根管銼表面殘留物清除效果的研究目的:研究氬氣等離子體對(duì)根管銼表面殘留污染物的清除效果。方法:取經(jīng)過常規(guī)清洗、消毒流程的根管銼30根,分為對(duì)照組和4個(gè)實(shí)驗(yàn)組(n=6),對(duì)照組不做處理,實(shí)驗(yàn)組采用氬氣等離子體分別處理5min、10min、15min、20min。掃描電鏡觀察根管銼表面污染物的清除情況,并對(duì)根管銼表面污染物做EDX分析。結(jié)果:氬氣等離子體處理20min能有效清除根管銼表面的污染物。結(jié)論:氬氣等離子體能有效清除根管銼表面殘留的污染物,可用于口腔診療中根管銼的表面清理。
[Abstract]:The first part of the study on killing Candida albicans on resin base surface by plasma. Experiment on killing Candida albicans on resin base surface by plasma. Objective: to compare argon gas with 30H _ 2O _ 2 and 50H _ 2O _ 2 plasma on resin base. Killing effect of Candida albicans on Surface plankton methods: 56 specimens of 9 mm 脳 9 mm 脳 2 mm were made from polymethyl methacrylate material. After being cultured in 1.0 脳 10 8 CFU / ml Candida albicans PBS suspension for 2 hours, we took out the light bleaching of Candida albicans for 2 hours and divided them into 14 groups. The control group was untreated, and the argon plasma was treated with 20 s ~ 40 s ~ (60 s) ~ (60 s) ~ (60 sL) ~ (80 sL) ~ (30) H ~ (2 +) C ~ (2 +) O _ (2) and 50 ~ (10) H _ 2O _ (2) plasma for 5 s ~ (10) s ~ (10) s ~ (-1) ~ 15 s ~ (20) s ~ (20) s respectively. The PBS solution was eluted and removed after the treatment. Series dilution, coating plate .48h, count colony forming units, calculate logarithmic value of kill. Referring to European standard en 1275-2005, the fungal reduction of 4 log10CFU after treatment is regarded as effective. Results: argon plasma treatment of 100s ~ 30H _ 2O _ 2 and 50H _ 2O _ 2 plasma treatment for 15s, The number of Candida albicans on the base surface was reduced by 4 log10 CFU and the number of Candida albicans was reduced by 4 log10 CFU. Conclusion: the plasma can effectively kill Candida albicans. 30H _ 2O _ 2 and 50H _ 2O _ 2 plasma in Argon base base base. Conclusion: the plasma can kill effectively the bactericidal properties of the plasma of Argon base base surface of Candida albicans .30H _ 2O _ 2 and 50H _ 2O _ 2. Experiments on killing Candida albicans Biofilm on Resin-base Surface by Plasma compared with Argon Plasma objective: to evaluate the efficacy of Argon 30H _ 2O _ 2 and H _ 2O _ 2 Plasma on Candida albicans in Biofilm on Resin-base Surface. Methods: the material of polymethyl methacrylate was made into 68 pieces of specimen of 9 mm 脳 9 mm 脳 2 mm. Candida albicans biofilm was cultured on the surface of the specimen for 48 h. 64 specimens were randomly divided into control group and 15 treatment groups. The control group was not treated, and the argon plasma was treated with 60 sn 120 s1 180 s20 s20 s 40 s, 60 s0 60 s0 60 s1 80 s10 100 s, respectively. Series dilution, coating plate .48h, counting colony forming units, calculating logarithmic value of killing, evaluating bactericidal effect. Referring to European standard en 1275-2005, the fungus reduction of 4 log10CFU after treatment was regarded as effective. The remaining 4 biofilm specimen: 1 as control group, The other three pieces were treated with Argon 30 and 50 H2O2 plasma respectively for 300 s. The damage of Candida albicans in the biofilm was observed by scanning electron microscope. Results: after argon plasma treatment with 30% H2O2 plasma, the plasma was treated with 100 sg 50 H 2O 2 plasma for 80 s, and after the treatment of 50 H 2O 2 plasma for 80 s, the damage of Candida albicans in the biofilm was observed by SEM. The number of Candida albicans in biofilm on the base surface decreased by 4 log10 CFU. The surface of cell membrane treated by plasma under electron microscope was rough and porous. Conclusion: the plasma can effectively kill Candida albicans in the biofilm. The second part of argon plasma can kill Candida albicans in biofilm more effectively than argon plasma can kill Candida albicans biofilm with the plasma of 50 H2O2 and the plasma of 50 H2O2 can kill the biofilm of Candida albicans more effectively than that of Argon gas, the second part of argon can kill Candida albicans biofilm more effectively than Argon plasma. Study on the removal effect of gas plasma on the surface residue of root canal file objective: to study the effect of argon plasma on the removal of residual pollutants on the surface of root canal file. Thirty root canal files in the disinfection process were divided into control group and 4 experimental groups. The control group was not treated, the experimental group was treated with argon plasma for 5 min, 10 min, 15 min and 20 min, respectively. The surface contamination removal of root canal file was observed by scanning electron microscope (SEM). The surface contamination of root canal file was analyzed by EDX. Results: argon plasma treatment for 20 minutes could effectively remove the pollutants on the root canal file surface. Conclusion: argon plasma can effectively remove the residual pollutants on the root canal file surface. Can be used in oral diagnosis and treatment of root canal file surface cleaning.
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類號(hào)】：R783

【參考文獻(xiàn)】

相關(guān)期刊論文 前4條

1 何智妍;王玉霞;黃正蔚;;變形鏈球菌luxS基因高表達(dá)對(duì)生物膜形成的影響[J];中華口腔醫(yī)學(xué)雜志;2015年09期

2 張燕萍;周培剛;吳鳳鳴;;義齒清潔劑對(duì)熱固化型基托樹脂物理及機(jī)械性能的影響[J];口腔醫(yī)學(xué);2014年12期

3 錢雅潔;蔣文暉;王翔;段寧;黃曉峰;王文梅;;白色念珠菌白斑中核苷酸結(jié)合寡聚化結(jié)構(gòu)域1、核因子κB及人β-防御素的表達(dá)[J];中華口腔醫(yī)學(xué)雜志;2014年08期

4 李金華;馬長(zhǎng)柏;張雄;;聚甲基丙烯酸樹脂義齒基托消毒的研究進(jìn)展[J];口腔醫(yī)學(xué);2013年08期

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