本文關(guān)鍵詞： 牙齦卟啉單胞菌 血管內(nèi)膜粘附因子 金屬蛋白酶 動(dòng)脈粥樣硬化　出處：《青島大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：通過給大鼠注射牙齦卟啉單胞菌(為牙周病的主要致病菌),觀察其對(duì)血管內(nèi)膜粘附因子和金屬蛋白酶的影響,證實(shí)牙周疾病為動(dòng)脈粥樣硬化斑塊形成的可能機(jī)制之一。 方法：120只大鼠隨機(jī)分為對(duì)照組、低劑量組和高劑量組,各40只,肌注牙齦卟啉單胞菌(Pg),每3天注射一次,且均不進(jìn)行抗生素干預(yù),歷時(shí)12周。取其靜脈血,檢測三組血液中的細(xì)胞間粘附分子-1、血管細(xì)胞粘附分子-1、基質(zhì)金屬蛋白酶-3及基質(zhì)金屬蛋白酶-9的水平。 結(jié)果：與對(duì)照組相比,低劑量組與高劑量組大鼠靜脈血中ICAM-1[(175.79±14.30)ng/ml比(182.62±15.07)ng/ml.(189.39±14.93)ng/ml].VCAM-1[(256.49±37.17)ng/ml比(271.58±32.85)ng/ml.(286.66±30.66)ng/ml] MMP-3[3.23±0.69) ng/ml比(3.61±0.8)ng/ml.(3.97±0.83)ng/ml]及MMP-9[(1.30±0.39)mg/L比(1.48±0.39)mg/L、(1.67±0.45)mg/L]水平明顯升高(P均0.05),且與低劑量組相比,高劑量組上述因子水平明顯升高,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。 結(jié)論：牙齦卟啉單胞菌會(huì)導(dǎo)致大鼠血清中細(xì)胞間粘附分子-1、血管細(xì)胞粘附分子-1、基質(zhì)金屬蛋白酶-3及基質(zhì)金屬蛋白酶-9含量的增加,而粘附分子可促進(jìn)炎癥的發(fā)生,基質(zhì)金屬蛋白酶可以降低彈性蛋白與膠原蛋白的含量,又可使包裹膽固醇的纖維帽分解、破裂,增加了冠心病的患病風(fēng)險(xiǎn)。進(jìn)一步說明P.gingivalis的感染可以促進(jìn)冠狀動(dòng)脈粥樣硬化性心臟病。
[Abstract]:Objective: to investigate the effects of porphyromonas gingivalis (Porphyromonas gingivalis) on vascular intima adhesion factor (intima) and metalloproteinase (MMP) in rats, and to confirm that periodontal disease is one of the possible mechanisms of atherosclerotic plaque formation. Methods one hundred and twenty rats were randomly divided into control group, low-dose group and high-dose group, 40 rats in each group, injected intramuscularly with Porphyromonas gingivalis once every 3 days without antibiotic intervention for 12 weeks. The levels of intercellular adhesion molecule-1, vascular cell adhesion molecule-1, matrix metalloproteinase-3 and matrix metalloproteinase-9 were measured in three groups. Results: compared with the control group, ICAM-1 (175.79 鹵14.30 ng / ml vs 182.62 鹵15.07 ng / ml vs 182.62 鹵15.07 鹵14.93 ng / ml) .VCAM-1 [256.49 鹵37.17 ng / ml vs 271.58 鹵32.85 ng / ml vs 271.58 鹵32.85 ng / ml] MMP-3 [3.23 鹵0.69 ng/ml vs 3.61 鹵0.83ngg. ml] and MMP-9 [1.30 鹵0.39 mg / L = 1.48 鹵0.39 mg / L] the level of MMP-3 [3.23 鹵0.69 ng/ml vs 3.61 鹵0.83ngg. ml] and MMP-9 [1.30 鹵0.39 mg / L = 1.48 鹵0.39 鹵0.39 mg / L] significantly increased compared with the control group, and increased significantly compared with the above dosage group, and the dose was significantly higher than that in the control group (3.23 鹵0.69 vs 182.62 鹵15.07 ng / ml vs 182.62 鹵15.07 鹵14.93 鹵14.93 ng / ml vs 271.58 鹵32.85 ng / ml vs 271.58 鹵32.85 ng / ml vs 3.23 鹵0.69 vs 3.61 鹵0.69 vs 3.61 鹵0.83ngg. / ml) and MMP-9 (1.30 鹵0.39 mg / L vs 1.48 鹵0.39 mg / ml vs 271.58 鹵32.85 ng / ml vs 271.58 鹵32.85 ng / ml). The difference was statistically significant (P 0.05). Conclusion: porphyromonas gingivalis can increase the contents of intercellular adhesion molecule -1, vascular cell adhesion molecule -1, matrix metalloproteinase-3 and matrix metalloproteinase-9 in serum of rats. Matrix metalloproteinases reduce the content of elastin and collagen, and break down the fibrous cap that encapsulates cholesterol. The infection of P.gingivalis may promote coronary atherosclerosis heart disease.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R541.4;R781.4

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