本文關鍵詞： 牙齒缺失 學習記憶 海馬組織 細胞凋亡　出處：《新疆醫(yī)科大學》2017年碩士論文　論文類型：學位論文

【摘要】：目的:觀察磨牙缺失后對幼年大鼠海馬CA1區(qū)神經元細胞凋亡及海馬神經元中Caspase-3的表達的影響,以探討磨牙缺失幼年大鼠海馬神經細胞凋亡可能的機制。方法:雄性SD大鼠,4-5周齡60只,按完全隨機設計平均分成對照組、實驗組(磨牙缺失組)(n=30),再依據造模后斷頭取腦時間將30只大鼠隨機分成4周組、8周組、20周組,采用HE染色及免疫組織化學法檢測Caspase-3蛋白的表達情況及末端標記法(TUNEL)法觀察大鼠腦內神經細胞凋亡的變化。結果:1.對照組海馬CA1區(qū)細胞形態(tài)結構正常,無明顯病理性改變。實驗組細胞出現(xiàn)腫脹、空泡,結構排列紊亂,神經元細胞明顯減少。2.在4周、8周、20周,對照組Caspase-3無統(tǒng)計學差異(P=0.301),實驗組海馬CA1區(qū)Caspase-3陽性細胞數增加,差異有統(tǒng)計學意義(P=0.000),任意兩組相比Caspase-3表達差異均有統(tǒng)計學意義,P0.001;3.實驗組TUNEL陽性細胞數有統(tǒng)計學差異(P=0.000),對照組TUNEL陽性細胞數無統(tǒng)計學差異(P=0.276)任意兩組相比TUNEL陽性細胞數表達差異均有統(tǒng)計學意義(P0.001)。結論:長時期咀嚼刺激減少會導致大鼠海馬區(qū)細胞凋亡增多,同時可上調Caspase-3蛋白的表達,提示長時期磨牙缺失誘導加快致發(fā)育期的大鼠海馬組織細胞發(fā)生凋亡。
[Abstract]:Aim: to observe the effect of molar tooth loss on apoptosis of hippocampal CA1 neurons and expression of Caspase-3 in hippocampal neurons of young rats. To explore the possible mechanism of neuronal apoptosis in hippocampal neurons of young rats with molar absence methods: 60 male SD rats aged 4-5 weeks were divided into two groups according to the complete randomized design. In the experimental group, 30 rats were randomly divided into 4 weeks group, 8 weeks group and 20 weeks group. The expression of Caspase-3 protein and the changes of neuronal apoptosis in rat brain were detected by HE staining and immunohistochemical method. Results: 1. The morphology and structure of hippocampal CA1 cells in the control group were normal. There was no obvious pathological change. In the experimental group, there were swelling, vacuole, disorder of structure and decrease of neuronal cells. After 4 weeks, 8 weeks and 20 weeks, there was no significant difference in Caspase-3 in the control group (P < 0. 301). The number of Caspase-3 positive cells in the hippocampal CA1 area of the experimental group was increased. There was significant difference in the expression of Caspase-3 between any two groups. There was a statistical difference in the number of TUNEL positive cells in the experimental group and in the control group. There was no statistical difference in the number of TUNEL positive cells in the control group (P < 0. 276). The number of TUNEL positive cells in the experimental group was smaller than that in the TUNEL group (P < 0. 276). Conclusion: the decrease of masticatory stimulation for a long period of time will lead to the increase of apoptosis in the hippocampus of rats. At the same time, the expression of Caspase-3 protein could be upregulated, suggesting that long term molar deletion could accelerate the apoptosis of hippocampal tissue cells in developing rats.
【學位授予單位】：新疆醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2017
【分類號】：R781

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