本文關(guān)鍵詞： 牙齒缺失 學(xué)習(xí)記憶 海馬組織 細(xì)胞凋亡　出處：《新疆醫(yī)科大學(xué)》2017年碩士論文　論文類(lèi)型：學(xué)位論文

【摘要】：目的:觀察磨牙缺失后對(duì)幼年大鼠海馬CA1區(qū)神經(jīng)元細(xì)胞凋亡及海馬神經(jīng)元中Caspase-3的表達(dá)的影響,以探討磨牙缺失幼年大鼠海馬神經(jīng)細(xì)胞凋亡可能的機(jī)制。方法:雄性SD大鼠,4-5周齡60只,按完全隨機(jī)設(shè)計(jì)平均分成對(duì)照組、實(shí)驗(yàn)組(磨牙缺失組)(n=30),再依據(jù)造模后斷頭取腦時(shí)間將30只大鼠隨機(jī)分成4周組、8周組、20周組,采用HE染色及免疫組織化學(xué)法檢測(cè)Caspase-3蛋白的表達(dá)情況及末端標(biāo)記法(TUNEL)法觀察大鼠腦內(nèi)神經(jīng)細(xì)胞凋亡的變化。結(jié)果:1.對(duì)照組海馬CA1區(qū)細(xì)胞形態(tài)結(jié)構(gòu)正常,無(wú)明顯病理性改變。實(shí)驗(yàn)組細(xì)胞出現(xiàn)腫脹、空泡,結(jié)構(gòu)排列紊亂,神經(jīng)元細(xì)胞明顯減少。2.在4周、8周、20周,對(duì)照組Caspase-3無(wú)統(tǒng)計(jì)學(xué)差異(P=0.301),實(shí)驗(yàn)組海馬CA1區(qū)Caspase-3陽(yáng)性細(xì)胞數(shù)增加,差異有統(tǒng)計(jì)學(xué)意義(P=0.000),任意兩組相比Caspase-3表達(dá)差異均有統(tǒng)計(jì)學(xué)意義,P0.001;3.實(shí)驗(yàn)組TUNEL陽(yáng)性細(xì)胞數(shù)有統(tǒng)計(jì)學(xué)差異(P=0.000),對(duì)照組TUNEL陽(yáng)性細(xì)胞數(shù)無(wú)統(tǒng)計(jì)學(xué)差異(P=0.276)任意兩組相比TUNEL陽(yáng)性細(xì)胞數(shù)表達(dá)差異均有統(tǒng)計(jì)學(xué)意義(P0.001)。結(jié)論:長(zhǎng)時(shí)期咀嚼刺激減少會(huì)導(dǎo)致大鼠海馬區(qū)細(xì)胞凋亡增多,同時(shí)可上調(diào)Caspase-3蛋白的表達(dá),提示長(zhǎng)時(shí)期磨牙缺失誘導(dǎo)加快致發(fā)育期的大鼠海馬組織細(xì)胞發(fā)生凋亡。
[Abstract]:Aim: to observe the effect of molar tooth loss on apoptosis of hippocampal CA1 neurons and expression of Caspase-3 in hippocampal neurons of young rats. To explore the possible mechanism of neuronal apoptosis in hippocampal neurons of young rats with molar absence methods: 60 male SD rats aged 4-5 weeks were divided into two groups according to the complete randomized design. In the experimental group, 30 rats were randomly divided into 4 weeks group, 8 weeks group and 20 weeks group. The expression of Caspase-3 protein and the changes of neuronal apoptosis in rat brain were detected by HE staining and immunohistochemical method. Results: 1. The morphology and structure of hippocampal CA1 cells in the control group were normal. There was no obvious pathological change. In the experimental group, there were swelling, vacuole, disorder of structure and decrease of neuronal cells. After 4 weeks, 8 weeks and 20 weeks, there was no significant difference in Caspase-3 in the control group (P < 0. 301). The number of Caspase-3 positive cells in the hippocampal CA1 area of the experimental group was increased. There was significant difference in the expression of Caspase-3 between any two groups. There was a statistical difference in the number of TUNEL positive cells in the experimental group and in the control group. There was no statistical difference in the number of TUNEL positive cells in the control group (P < 0. 276). The number of TUNEL positive cells in the experimental group was smaller than that in the TUNEL group (P < 0. 276). Conclusion: the decrease of masticatory stimulation for a long period of time will lead to the increase of apoptosis in the hippocampus of rats. At the same time, the expression of Caspase-3 protein could be upregulated, suggesting that long term molar deletion could accelerate the apoptosis of hippocampal tissue cells in developing rats.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2017
【分類(lèi)號(hào)】：R781

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