本文關(guān)鍵詞： 牙髓干細(xì)胞 SOX 神經(jīng)分化 神經(jīng)前體細(xì)胞 細(xì)胞增殖 細(xì)胞治療　出處：《吉林大學(xué)學(xué)報(醫(yī)學(xué)版)》2016年06期 　論文類型：期刊論文

【摘要】：目的:探討轉(zhuǎn)錄因子SOX2對牙髓干細(xì)胞(DPSCs)神經(jīng)分化的影響,為研究DPSCs神經(jīng)分化的機(jī)制提供依據(jù)。方法:從人源第三磨牙牙髓中分離DPSCs(DPSCs組),通過逆轉(zhuǎn)錄病毒感染方法構(gòu)建過表達(dá)SOX2的DPSCs(DPSCs-SOX2組),以空白載體感染的DPSCs作為對照組(DPSCs-vector組),采用神經(jīng)分化培養(yǎng)基誘導(dǎo)各組DPSCs分化為神經(jīng)前體細(xì)胞(NPCs)。采用CCK-8法分析各組NPCs的增殖指數(shù)(PI);采用qPCR和流式細(xì)胞術(shù)檢測各組NPCs中Nestin和Pax6基因的表達(dá)水平;采用免疫熒光和流式細(xì)胞術(shù)分析各組NPCs在神經(jīng)元分化方面的能力和分化后β3-Tubulin的表達(dá)效率。結(jié)果:通過神經(jīng)分化培養(yǎng)基的定向誘導(dǎo),正常DPSCs、DPSCs-vector和DPSCs-SOX2均可通過分化形成NPCs,但DPSCs-SOX2組NPCs的PI和NPCs中Nestin和Pax6表達(dá)水平明顯高于DPSCs組和DPSCs-vector組(P0.05);DPSCs-SOX2組NPCs在神經(jīng)元分化方面也具有一定的優(yōu)勢,分化后細(xì)胞的β3-Tubulin表達(dá)效率明顯高于其他2組(P0.05)。結(jié)論:SOX2對DPSCs的神經(jīng)分化具有一定的促進(jìn)作用。
[Abstract]:Objective: to investigate the effect of transcription factor SOX2 on neural differentiation of dental pulp stem cells (DPSCs). Methods: DPSCs(DPSCs group was isolated from the pulp of human third molar tooth. DPSCs(DPSCs-SOX2 group which expressed SOX2 was constructed by retrovirus infection. DPSCs infected by blank vector was used as control group. The proliferation index of NPCs in each group was analyzed by CCK-8 method, and the expression of Nestin and Pax6 genes in NPCs were detected by qPCR and flow cytometry. The ability of NPCs in neuronal differentiation and the expression efficiency of 尾 3-tubulin after differentiation were analyzed by immunofluorescence and flow cytometry. The expression of Nestin and Pax6 in NPCs in DPSCs-SOX2 group was significantly higher than that in DPSCs group and DPSCs-vector group (P 0.05). The expression of Nestin and Pax6 in NPCs of DPSCs-SOX2 group was significantly higher than that of DPSCs group and DPSCs-vector group (P 0.05) and DPSCs-SOX2 group had some advantages in neuronal differentiation, but the expression of Nestin and Pax6 in NPCs of DPSCs-SOX2 group was significantly higher than that in DPSCs group and DPSCs-vector group. The expression efficiency of 尾 _ 3-tubulin in differentiated cells was significantly higher than that in the other two groups (P _ (0.05)). Conclusion: the expression of 尾 _ 3-tubulin in differentiated cells can promote the neural differentiation of DPSCs to some extent.
【作者單位】： 暨南大學(xué)第二臨床醫(yī)學(xué)院深圳市人民醫(yī)院麻醉科;吉林大學(xué)藥學(xué)院再生醫(yī)學(xué)系;
【基金】：吉林省科技廳科技發(fā)展計劃項目資助課題(20120955) 廣東省深圳市科技局知識創(chuàng)新計劃資助課題(JCYJ20140416122812052) 廣東省深圳市衛(wèi)計委衛(wèi)生計生系統(tǒng)科研項目資助課題(201401010) 吉林大學(xué)研究生創(chuàng)新基金資助課題(2015009)
【分類號】：R781

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