本文關(guān)鍵詞： 低溫等離子體 低溫等離子體活性水 致病菌 生物膜 活性氧　出處：《蘭州大學(xué)》2014年碩士論文　論文類型：學(xué)位論文

【摘要】：低溫等離子體因其可以有效的殺滅存在于空氣中以及液體中的致病菌,而逐漸被眾多學(xué)者所認(rèn)可。低溫等離子體活性水作為低溫等離子體一種新的殺菌形式出現(xiàn),其殺菌作用以及殺菌機(jī)理還處于初步階段,這需要我們進(jìn)一步去研究發(fā)現(xiàn)。 目的：通過(guò)低溫等離子體活性水處理金黃色葡萄球菌、大腸桿菌、變形鏈球菌、白色念珠菌及變形鏈球菌生物膜,探討低溫等離子體活性水的殺菌效應(yīng)以及殺菌機(jī)理。 方法：通過(guò)直流大氣壓Ar/O2(2%)低溫等離子體處理20mL蒸餾水20min,來(lái)制備低溫等離子體活性水。制備完成的低溫等離子體活性水即刻處理金黃色葡萄球菌,大腸桿菌,變形鏈球菌,白色念珠菌和變形鏈球菌生物膜。菌落形成單位數(shù)和熒光染色評(píng)價(jià)低溫等離子體活性水的殺菌效果。用掃描電子顯微鏡觀察變形鏈球菌游離菌和變形鏈球菌生物膜的表面形態(tài)結(jié)構(gòu)。共聚焦激光掃描顯微鏡用來(lái)顯示低溫等離子體活性水對(duì)變形鏈球菌生物膜的殺菌效果。過(guò)氧化氫檢測(cè)試劑盒和發(fā)射光譜儀檢測(cè)低溫等離子體活性水中的活性物質(zhì),并分析討論低溫等離子體活性水的殺菌機(jī)理。 結(jié)果：菌落計(jì)數(shù)法、熒光染色法的檢測(cè)結(jié)果：低溫等離子體活性水在20min內(nèi)可以有效的殺滅金黃色葡萄球菌、大腸桿菌和變形鏈球菌,對(duì)白色念珠菌及變形鏈球菌生物膜的殺滅作用甚微；掃描電子顯微鏡顯示我們成功建立了變形鏈球菌生物膜模型,并通過(guò)共聚焦激光掃描顯微鏡觀測(cè)到生物膜的三維立體結(jié)構(gòu)；在波長(zhǎng)為777.2nm處可以清楚地觀察到原子氧的存在,并在低溫等離子體活性水中檢測(cè)到濃度為3.0mg/L的過(guò)氧化氫,而這些活性物質(zhì)可能在PAW殺菌過(guò)程中起著不同的作用. 結(jié)論：低溫等離子體活性水可以有效的殺滅細(xì)菌(金黃色葡萄球菌、大腸桿菌和變形鏈球菌),但是對(duì)真菌以及變形鏈球菌生物膜的殺菌作用較弱。
[Abstract]:Low temperature plasma has been recognized by many scholars because it can effectively kill pathogenic bacteria in air and liquid. Low temperature plasma active water as a new form of sterilization of low temperature plasma appears. Its bactericidal action and mechanism are still in the preliminary stage, which needs to be further studied and discovered. Objective: to study the bactericidal effect and bactericidal mechanism of low temperature plasma active water in treating the biofilms of Staphylococcus aureus, Escherichia coli, Streptococcus mutans, Candida albicans and Streptococcus mutans. Methods: 20 mL distilled water was treated with low temperature plasma at DC atmospheric pressure for 20 min to prepare low temperature plasma active water. The finished low temperature plasma active water was immediately treated with Staphylococcus aureus, Escherichia coli, Streptococcus mutans. Candida albicans and Streptococcus mutans biofilms. Colony formation units and fluorescent staining to evaluate the bactericidal efficacy of low temperature plasma active water. Observation of streptococcus mutans free bacteria and Streptococcus mutans by scanning electron microscopy. Surface morphology of the film. Confocal laser scanning microscopy is used to show the bactericidal effect of low temperature plasma active water on Streptococcus mutans biofilms. Hydrogen peroxide detection kit and emission spectrometer for low temperature plasma detection. Active substances in active water, The bactericidal mechanism of low temperature plasma active water was analyzed and discussed. Results: the results of colony counting and fluorescent staining showed that low temperature plasma active water could effectively kill Staphylococcus aureus, Escherichia coli and Streptococcus mutans within 20 minutes. The biofilm of Candida albicans and Streptococcus mutans had little effect on killing, scanning electron microscope showed that we had successfully established the biofilm model of Streptococcus mutans, and the three-dimensional structure of biofilm was observed by confocal laser scanning microscope. The existence of atomic oxygen can be clearly observed at 777.2 nm and the concentration of hydrogen peroxide is 3.0 mg / L in low temperature plasma active water. These active substances may play different roles in the sterilization of PAW. Conclusion: low temperature plasma active water can effectively kill bacteria (Staphylococcus aureus, Escherichia coli and Streptococcus mutans), but the bactericidal effect on fungi and Streptococcus mutans biofilms is weak.
【學(xué)位授予單位】：蘭州大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R780.2

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本文編號(hào)：1504525