本文關(guān)鍵詞：富血小板纖維蛋白和濃縮生長因子對施萬細胞影響的比較　出處：《中國醫(yī)科大學學報》2016年12期 　論文類型：期刊論文

  更多相關(guān)文章： 富血小板纖維蛋白 濃縮生長因子 施萬細胞 神經(jīng)生長因子 細胞周期

【摘要】：目的以施萬細胞作為周圍神經(jīng)模型,比較富血小板纖維蛋白(PRF)與濃縮生長因子(CGF)對其的影響。方法隨機選取18~55歲身體健康志愿者10人,無菌條件下采集靜脈血10 m L,制備PRF和CGF。將細胞隨機分為對照組、PRF組和CGF組。倒置相差顯微鏡下觀察細胞形態(tài);MTT比色法測定細胞增殖能力;酶聯(lián)免疫吸附法檢測細胞上清液中神經(jīng)生長因子的分泌量;流式細胞儀檢測細胞周期。結(jié)果倒置相差顯微鏡下觀察各組細胞形態(tài)無明顯差異;MTT結(jié)果顯示PRF組和CGF組與對照組相比光密度值明顯升高(P0.05),細胞上清液中神經(jīng)生長因子分泌量均明顯升高(P0.05),處于S+G2M期的細胞數(shù)明顯增多(P0.01),而PRF組與CGF組相比各項指標的差異無統(tǒng)計學意義。結(jié)論 PRF和CGF均可以促進施萬細胞增殖并增加神經(jīng)生長因子的分泌量,但就改善細胞增殖能力、促進神經(jīng)生長因子分泌來看,二者無明顯差異。
[Abstract]:Objective to use Schwann cells as peripheral nerve model. The effects of platelet rich fibrin (PRF) and concentrated growth factor (CGF) on it were compared. Methods 10 healthy volunteers aged from 18 to 55 years were randomly selected. PRF and CGF were prepared by collecting 10 mL venous blood under aseptic condition. The cells were randomly divided into two groups: control group and CGF group. The morphology of the cells was observed under inverted phase contrast microscope. Cell proliferation was measured by MTT colorimetry. Enzyme linked immunosorbent assay (Elisa) was used to detect the secretion of nerve growth factor (NGF) in the supernatant. Results there was no significant difference in cell morphology under inverted phase contrast microscope. MTT results showed that the optical density of PRF group and CGF group was significantly higher than that of control group, and the secretion of nerve growth factor in cell supernatant was significantly higher than that of control group (P 0.05). The number of cells in S G 2m phase increased significantly (P 0.01). Conclusion both PRF and CGF can promote the proliferation of Schwann cells and increase the secretion of nerve growth factor. However, there was no significant difference between the two groups in terms of improving cell proliferation and promoting the secretion of nerve growth factor (NGF).
【作者單位】： 錦州醫(yī)科大學附屬第二醫(yī)院口腔種植中心;中國醫(yī)科大學附屬盛京醫(yī)院集團撫順醫(yī)院口腔科;
【基金】：遼寧省科技廳優(yōu)秀人才培育項目(2014022025)
【分類號】：R783.6
【正文快照】： 113000)口腔種植技術(shù)正處于飛速發(fā)展與日益完善的黃金時期,越來越多的牙齒缺失患者放棄了傳統(tǒng)鑲牙而選擇口腔種植修復(fù),后者無論是在美觀還是實用性上與傳統(tǒng)鑲牙相比都有較大優(yōu)勢。牙周膜組織中含有大量魯菲尼小體,它們是天然牙的機械感受器,對于牙齒缺失的患者來說,牙周膜組織，

本文編號：1436006