本文關(guān)鍵詞：RANKL和OPG對(duì)不同骨移植材料行位點(diǎn)保存中骨構(gòu)建的作用　出處：《新疆醫(yī)科大學(xué)》2015年碩士論文　論文類型：學(xué)位論文

  更多相關(guān)文章： 拔牙位點(diǎn)保存 骨移植 骨保護(hù)因子 核因子kB受體活化因子配體 破骨細(xì)胞

【摘要】：目的:通過(guò)觀察破骨細(xì)胞核因子kB受體活化因子配體和骨保護(hù)因子在使用不同骨移植材料行拔牙位點(diǎn)保存時(shí)骨構(gòu)建過(guò)程中的表達(dá)變化,探討破骨細(xì)胞對(duì)不同骨移植材料植入拔牙窩內(nèi)在引導(dǎo)骨再生中的作用與意義。材料及方法:選取六只12月齡雄性beagle犬,微創(chuàng)拔除其上下頜雙側(cè)第一側(cè)切牙,將四個(gè)位點(diǎn)隨機(jī)做不同處理,分組為:A組:填入富血小板纖維蛋白(Platelet-rich fibrin,PRF),B組:填入骨誘導(dǎo)活性材料(osteoinduction active material,OAM),C組:填入羥基磷灰石生物陶瓷(coralline hydroxyapatite,CHA),D組:空置(對(duì)照)。六只犬隨機(jī)分別于術(shù)后4周、術(shù)后12周各處死3只,制作牙槽骨標(biāo)本。采用免疫組化技術(shù)檢測(cè)24塊拔牙位點(diǎn)保存制作的犬牙槽骨標(biāo)本中RANKL與OPG的表達(dá)變化。結(jié)果:4周處死標(biāo)本骨組織中OPG的表達(dá)A組依次高于B組,C組和D組(P0.05),RANKL的表達(dá)D組依次高于C組,B組和A組(P0.05),12周處死標(biāo)本骨組織中OPG的表達(dá)D組依次高于C組,B組和A組(P0.05)。RANKL的表達(dá)C組依次高于B組,A組和D組(P0.05)。結(jié)論:骨移植材料在骨改建過(guò)程中通過(guò)促進(jìn)成骨細(xì)胞分化成熟而刺激OPG表達(dá)升高,抑制破骨細(xì)胞,從而加強(qiáng)成骨作用,有效提高新骨形成量,提示骨移植材料通過(guò)調(diào)控RANKL和OPG在拔牙窩新骨形成的過(guò)程中發(fā)揮著重要的作用。
[Abstract]:Objective: to observe the expression changes of osteoclast nuclear factor kB receptor activating factor ligand and bone protection factor during bone construction during the preservation of extraction sites using different bone graft materials. To explore the role and significance of osteoclasts in guiding bone regeneration by implantation of different bone graft materials into extraction fossa. Materials and methods: six 12-month-old male beagle dogs were selected. The maxillary and mandibular first incisors were extracted by minimally invasive extraction, and the four sites were randomly divided into two groups: group A: Platelet-rich fibrin filled with platelet-rich fibrin. Group B: osteoinductive active material (OAM). Group C: hydroxyapatite bioceramics: group D: empty (control group): 6 dogs were randomly assigned 4 weeks after operation. Three rats died 12 weeks after operation. The expression of RANKL and OPG in 24 canine alveolar bone specimens preserved at extraction sites were detected by immunohistochemical technique. The expression of OPG in bone tissue of group A was higher than that of group B at 4 weeks. The expression of OPG in group D was higher than that in group C (P 0.05) and group A (P 0.05) at 12 weeks. The expression of OPG in group D was higher than that in group C (P 0.05). The expression of P0.05, RANKL in group B and group A was higher than that in group B, respectively. Conclusion: during bone remodeling, bone graft materials stimulate the expression of OPG and inhibit osteoclasts in the process of bone remodeling by promoting the differentiation and maturation of osteoblasts, thus enhancing the osteogenic effect. It is suggested that bone graft material plays an important role in the formation of new bone in the extraction of dental fossa by regulating RANKL and OPG.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2015
【分類號(hào)】：R782.11
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本文編號(hào)：1416746