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  本文關(guān)鍵詞：TLR2和TLR4在人慢性根尖周病組織肥大細胞上的表達　出處：《中國病理生理雜志》2016年03期 　論文類型：期刊論文

  更多相關(guān)文章： 慢性根尖周病 肥大細胞 Toll樣受體 Toll樣受體

【摘要】：目的:觀察人不同類型慢性根尖周病組織中肥大細胞(mast cells,MCs)上Toll樣受體2(Toll-like receptor 2,TLR2)和TLR4的表達情況,探討TLR2-類胰蛋白酶(tryptase)和TLR4-tryptase雙陽性MCs在慢性根尖周疾病發(fā)病機制中的作用。方法:將60例自愿參與本研究的受試者按根尖周病分類標(biāo)準分為3組:(1)正常對照組;(2)根尖周肉芽腫組;(3)根尖周囊腫組。將根尖周組織標(biāo)本置于4%甲醛固定液中浸泡48 h以上,制作連續(xù)組織切片。HE染色,光學(xué)顯微鏡下觀察各組根尖周標(biāo)本的組織學(xué)變化;免疫熒光雙染色后于熒光顯微鏡下觀察TLR2-tryptase和TLR4-tryptase雙陽性MCs在根尖周組織中的分布情況。結(jié)果:根尖周病變組TLR2-tryptase和TLR4-tryptase雙陽性MCs比正常牙周膜組明顯增多(P0.01);與根尖肉芽腫組相比,根尖囊腫組TLR2-tryptase及TLR4-tryptase雙陽性MCs數(shù)目明顯增高(P0.01)。結(jié)論:TLR2及TLR4在慢性根尖周疾病組織中MCs上表達增加,提示TLR2-tryptase及TLR4-tryptase雙陽性MCs可能參與慢性根尖周病發(fā)病過程的免疫調(diào)節(jié)。
[Abstract]:Objective: to observe the Toll like receptor 2 Toll-like receptor 2 on mast cells of different types of chronic periapical disease. TLR2) and TLR4 expression. Study on TLR2-Tryptase. Methods: 60 subjects who volunteered to participate in this study were divided into 3 groups according to the classification criteria of periapical disease. 1) normal control group; 2) periapical granuloma group; 3) periapical cyst group. The periapical tissue samples were soaked in 4% formaldehyde fixative solution for more than 48 hours, and the tissue sections were stained with HE. The histological changes of periapical specimens were observed under optical microscope. The distribution of TLR2-tryptase and TLR4-tryptase double positive MCs in periapical tissues was observed under fluorescence microscope after double immunofluorescence staining. The number of TLR2-tryptase and TLR4-tryptase double positive MCs in periapical lesion group was significantly higher than that in normal periodontal ligament group. P0.01; Compared with the apical granuloma group. The number of TLR2-tryptase and TLR4-tryptase double positive MCs in the apical cyst group was significantly higher than that in the control group (P 0.01). Conclusion the expression of TLR2 and TLR4 in the tissues of chronic periapical diseases is increased. The results suggest that TLR2-tryptase and TLR4-tryptase double positive MCs may be involved in the immunomodulation of chronic periapical disease.
【作者單位】： 暨南大學(xué)醫(yī)學(xué)院口腔醫(yī)學(xué)系;
【基金】：廣東省社會發(fā)展領(lǐng)域科技計劃(No.2013B021800043;No.2014A020212212)
【分類號】：R781.34
【正文快照】： 慢性根尖周疾病是口腔常見疾病之一,主要由來源于感染根管的細菌及其代謝產(chǎn)物擴散至根尖周組織所致,表現(xiàn)為根尖周組織的炎癥與骨吸收。傳統(tǒng)認為,肥大細胞(mast cells,MCs)是過敏性炎癥反應(yīng)的主要效應(yīng)細胞,近年的研究發(fā)現(xiàn),MCs作為效應(yīng)和調(diào)節(jié)性免疫細胞在固有免疫的建立和適應(yīng)性，

本文編號：1413186