本文關鍵詞：應用于牙科種植體的新型鈦合金生物安全性檢測　出處：《吉林大學》2016年碩士論文　論文類型：學位論文

  更多相關文章： 生物安全性 鈦合金 種植體 細胞毒性 遺傳毒性

【摘要】：目的與背景:口腔種植體的出現(xiàn)里程碑式的改變了牙列缺損和牙列缺失的修復方式。骨結合(Osseointergration)是種植體能夠成功植入的理論基礎,其指骨和種植體之間達到無纖維組織的穩(wěn)定結合。為了能更好的達到骨結合,人們通過研發(fā)新型種植體材料及進行種植體表面改性來達到這一目的,隨著材料學的發(fā)展和進步,越來越多的材料應用于種植體領域,種植所應用的材料要求具有良好的生物相容性,良好的抗腐蝕性,骨結合性及機械性能,其中無毒金屬材料為種植體材料的首選。鈦金屬由于其出色的生物學性能,靈活性及抗腐蝕能力在臨床具有廣泛的應用,鋯,鉭,鈮已經(jīng)被證實具有出色的生物安全性和機械性能,對成骨細胞的增殖分泌具有促進作用。根據(jù)國際標準ISO-10993-5-2009和ISO-10993-3-2009中所描述的試驗方法:植入生物體內30天的材料,必須進行生物安全性檢測。本研究從對一種新型的含有鋯,鉭,鈮的鈦合金進行了體內及體外生物安全性的檢測。方法:本實驗采用華南理工大學提供的新型鈦合金鈦片。將陰性對照組選擇為DMEM高糖培養(yǎng)基組。按照ISO-10993-12-2012制備各組鈦片的浸提液,通過CCK-8實驗評價各實驗組鈦片的細胞毒性,應用單細胞凝膠電泳實驗檢測實驗組鈦片的遺傳毒性,評價各實驗組鈦片對成骨細胞DNA造成損傷的水平,通過皮下植入實驗和急性毒性實驗對各組鈦片的體內安全性進行檢測,對實驗結果分別進行大體觀察和組織學評價,通過SPSS軟件將各實驗組的實驗結果進行統(tǒng)計學分析。結果:1:CCK-8實驗結果:各實驗組試件對成骨細胞均不會產生細胞毒性,對細胞增殖無抑制作用;2.SCGE結果:各組實驗圖像數(shù)據(jù)的OTM:(Olive tail moment:尾部DNA占總DNA的百分比與頭、尾部中心間距的乘積)和%tail DNA(尾部DNA百分量,尾部和總彗星熒光百分比)與DMEM陰性對照組之間差異無顯著性;提示各實驗組,相對于純鈦,不會對成骨細胞造成DNA損傷,不具有遺傳毒性。3.皮下植入實驗結果:通過大體觀察可以看出商業(yè)純鈦和兩種鈦合金對動物均無刺激,周圍組織未見炎癥,組織學觀察可以看出Si組囊壁略不規(guī)整,有少量毛細血管充血。4.急性全身毒性實驗結果:急性全身毒性實驗結果:未見任何實驗動物表現(xiàn)出較對照組明顯的生物學反應,未見體重下降。結論:1.新型鈦合金無細胞毒性,不會抑制成骨細胞的生長;2.新型鈦合金對成骨細胞造成的DNA原始損傷與商業(yè)純鈦純鈦相同;3.新型鈦合金對不對皮膚產生毒性反應,沒有刺激作用,不產生炎癥及過敏反應。4.新型鈦合金無急性全身毒性。實驗提示新型鈦合金不具有細胞毒性、遺傳毒性以、植入后局部反應及急性全身毒性,該種植體具有良好的體內體外生物安全性,肯定了其將來應用于臨床的可能性,并為其提供了理論支持。
[Abstract]:Objective and background: the landmark appearance of dental implants has changed the repair of dentition defects and dentition. It is the theoretical basis for the successful implantation of implants. In order to achieve better osseointegration, people develop new implant materials and implant surface modification to achieve this goal. With the development and progress of material science, more and more materials are used in implant field. The materials used in implant are required to have good biocompatibility, good corrosion resistance, bone bonding and mechanical properties. Titanium is widely used in clinic because of its excellent biological properties, flexibility and corrosion resistance, zirconium and tantalum. Niobium has been proven to have excellent biological safety and mechanical properties. Promote the proliferation and secretion of osteoblasts. According to the test methods described in international standards ISO-10993-5-2009 and ISO-10993-3-2009:. The material implanted in the organism for 30 days. Biosafety tests must be performed. This study is based on a new type containing zirconium and tantalum. The biological safety of titanium alloy of niobium was determined in vivo and in vitro. Methods:. In this experiment, a new titanium alloy titanium tablet provided by South China University of Technology was used. The negative control group was selected as DMEM high sugar medium group. The extraction of each titanium slice was prepared according to ISO-10993-12-2012. Liquid. The cytotoxicity of titanium tablets in each experimental group was evaluated by CCK-8 assay, and the genotoxicity of titanium tablets in experimental group was detected by single cell gel electrophoresis (SCGE). To evaluate the level of DNA damage of osteoblasts induced by titanium tablets in each experimental group, and to detect the safety of titanium tablets in each group by subcutaneous implantation test and acute toxicity test. Gross observation and histological evaluation of the experimental results were carried out. The experimental results of each experimental group were statistically analyzed by SPSS software. Results: the results of 1: 1: CCK-8 experiment showed that no cytotoxicity to osteoblasts was found in all experimental groups. There was no inhibitory effect on cell proliferation. 2. SCGE results: OTM:(Olive tail momentum of each group of experimental image data: the percentage and head of tail DNA in total DNA. There was no significant difference between tail DNA (tail DNA 100 component, tail and total comet fluorescence percentage) and DMEM negative control group. These results suggest that the experimental group will not cause DNA damage to osteoblasts compared with pure titanium. Experimental results of subcutaneous implantation: commercial pure titanium and two kinds of titanium alloys were not irritated to animals, and no inflammation was found in the surrounding tissues. Histological observation shows that the cystic wall of Si group is slightly irregular. There was a small amount of capillary hyperemia. 4. Acute systemic toxicity test results: acute systemic toxicity test results: no experimental animals showed a more obvious biological response than the control group. Conclusion: the new titanium alloy has no cytotoxicity and does not inhibit the growth of osteoblasts. 2. The original damage of DNA to osteoblasts caused by new titanium alloy is the same as that of commercial pure titanium. 3. The new titanium alloy has no toxic reaction to the skin, no irritation, no inflammation and no allergic reaction. The new titanium alloy has no acute systemic toxicity. The experiment shows that the new titanium alloy has no cytotoxicity. Genetic toxicity, local reaction after implantation and acute systemic toxicity, the implant has good biological safety in vivo and in vitro, confirmed the possibility of its clinical application in the future, and provided theoretical support for it.
【學位授予單位】：吉林大學
【學位級別】：碩士
【學位授予年份】：2016
【分類號】：R783.6

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