【摘要】：目的觀察孤獨(dú)癥大鼠前額葉皮質(zhì)神經(jīng)元凋亡相關(guān)蛋白Caspase-3和Bcl-2表達(dá)的變化,探討細(xì)胞凋亡在孤獨(dú)癥發(fā)病中的作用。方法健康繁殖期Wistar雌鼠20只,體質(zhì)量250~260 g,隨機(jī)選取10只在E12.5腹腔注射丙戊酸鈉(VPA),其子代為孤獨(dú)癥模型組;其余10只在E12.5腹腔注射生理鹽水,其子代為對照組。通過比較負(fù)向性實(shí)驗(yàn)、自梳理實(shí)驗(yàn)驗(yàn)證模型是否成功;Western blotting方法對比對照組與孤獨(dú)癥模型組大鼠P42前額葉皮質(zhì)凋亡相關(guān)蛋白Caspase-3和Bcl-2表達(dá)的變化。結(jié)果成功建立孤獨(dú)癥動物模型。與對照組比較,孤獨(dú)癥模型組大鼠旋轉(zhuǎn)調(diào)頭時(shí)間顯著延長(P0.05),理毛時(shí)間顯著增加(P0.05);P42前額葉皮質(zhì)凋亡相關(guān)蛋白Caspase-3表達(dá)顯著降低(P0.05),Bcl-2表達(dá)顯著升高(P0.05)。結(jié)論孤獨(dú)癥大鼠P42前額葉皮質(zhì)凋亡受到抑制,提示調(diào)節(jié)凋亡可能是一個(gè)潛在的孤獨(dú)癥治療策略。
[Abstract]:Objective To observe the changes of the expression of Caspase-3 and Bcl-2 in the prefrontal cortex of autistic rats, and to explore the role of apoptosis in the pathogenesis of autism. Methods Twenty-two female Wistar rats were randomly selected to be injected with sodium valproate (VPA) in the abdominal cavity of E12.5. The rest of the 10 rats were injected with normal saline in the abdominal cavity of E12.5, and their children were in the control group. The expression of Caspase-3 and Bcl-2 in the prefrontal cortex of P42 in the control group and the autistic model group was compared by Western blotting. Results The autistic animal model was successfully established. The expression of Caspase-3 in the prefrontal cortex of P42 was significantly lower (P0.05), and the expression of Bcl-2 was significantly increased (P0.05). Conclusion The apoptosis of P42 prefrontal cortex of autistic rats is inhibited, and it is suggested that the regulation of apoptosis may be a potential autistic treatment strategy.
【作者單位】： 貴陽醫(yī)學(xué)院人體解剖學(xué)教研室;貴陽市第二人民醫(yī)院神經(jīng)內(nèi)科;貴陽醫(yī)學(xué)院附屬醫(yī)院兒科;貴陽醫(yī)學(xué)院生物技術(shù)教研室;
【基金】：貴州市科技計(jì)劃項(xiàng)目[筑科合同(2011103)18號] 貴州省科技攻關(guān)計(jì)劃課題[黔科合SY(2010)3082號]
【分類號】：R749.94

【參考文獻(xiàn)】

相關(guān)期刊論文 前2條

1 李瑞錫;江開達(dá);彭裕文;;孤獨(dú)癥研究新進(jìn)展[J];復(fù)旦學(xué)報(bào)(醫(yī)學(xué)版);2010年01期

2 譚迎花;郗春艷;汪永娟;王麗波;韓晶晶;;孤獨(dú)癥患兒頭圍測量分析[J];中國實(shí)用兒科雜志;2011年10期

【共引文獻(xiàn)】

相關(guān)期刊論文 前10條

1 張麗雯;;改善孤獨(dú)癥兒童目光接觸的個(gè)案研究[J];時(shí)代教育;2013年05期

2 李佩瓊;章鈞;章遠(yuǎn)志;;臨床醫(yī)生對兒童孤獨(dú)癥的了解狀況抽樣調(diào)查[J];重慶醫(yī)學(xué);2013年17期

3 宋翠娣;金星明;周念麗;段婭莉;;上海市浦東新區(qū)自閉癥兒童康復(fù)訓(xùn)練醫(yī)教結(jié)合模式探索與實(shí)踐[J];教育生物學(xué)雜志;2013年03期

4 Fatih Mehmet T，

本文編號：2447190