【摘要】：目的研究β片層阻斷肽H102對(duì)PAP雙轉(zhuǎn)基因小鼠腦內(nèi)ERK信號(hào)轉(zhuǎn)導(dǎo)通路的激活作用。方法將PAP雙轉(zhuǎn)基因小鼠隨機(jī)分為模型組和給藥組,每組10只,設(shè)同背景C57BL/6J小鼠為對(duì)照組。給藥組每日鼻腔給予H102溶液(5.8 mg/kg)5μL,對(duì)照組、模型組每日鼻腔給予等體積H102空白輔料溶液。30 d后行Morris水迷宮測(cè)試。之后采用免疫組化及免疫印跡技術(shù)觀察小鼠腦組織內(nèi)RAS、P-MEK及P-ERK蛋白的表達(dá)變化。結(jié)果 (1)Morris水迷宮測(cè)試。模型組小鼠學(xué)習(xí)記憶能力較對(duì)照組顯著降低,給藥組較模型組顯著提高(均P0.05)。(2)免疫組化及免疫印跡檢測(cè)結(jié)果。模型組腦內(nèi)RAS、P-MEK及P-ERK表達(dá)較對(duì)照組顯著降低,給藥組蛋白表達(dá)較模型組顯著增高(均P0.01)。結(jié)論β片層阻斷肽H102可激活PAP雙轉(zhuǎn)基因小鼠腦內(nèi)ERK信號(hào)轉(zhuǎn)導(dǎo)通路,增加神經(jīng)細(xì)胞PAS、P-MEK及P-ERK的含量,改善PAP小鼠學(xué)習(xí)記憶能力。
[Abstract]:Aim to study the effect of 尾-lamellar blocking peptide H102 on the activation of ERK signal transduction pathway in the brain of PAP double transgenic mice. Methods PAP double transgenic mice were randomly divided into two groups: model group (n = 10) and administration group (n = 10). C57BL/6J mice with the same background were used as control group. The rats in the treatment group were given 5 渭 L H102 solution (5.8 mg/kg) per day, and the control group and the model group were given the same volume of H102 blank adjunct solution every day. After 30 days, the Morris water maze test was performed. Immunohistochemical and immunoblotting techniques were used to observe the expression of RAS,P-MEK and P-ERK protein in the brain tissue of mice. Results (1) Morris water maze test. The learning and memory ability of the model group was significantly lower than that of the control group, and the results of immunohistochemistry and immunoblotting were significantly improved in the administration group than in the model group (P0.05). (2). The expression of RAS,P-MEK and P-ERK in the brain of the model group was significantly lower than that of the control group, and the expression of the protein in the administration group was significantly higher than that of the model group (P0.01). Conclusion 尾-lamellar blocking peptide H102 can activate the ERK signal transduction pathway in the brain of PAP double transgenic mice, increase the contents of PAS,P-MEK and P-ERK in nerve cells, and improve the learning and memory ability of PAP mice.
【作者單位】： 天津醫(yī)科大學(xué)生理教研室;天津醫(yī)科大學(xué)內(nèi)分泌研究所;
【基金】：國(guó)家科技重大專項(xiàng)基金資助項(xiàng)目(2009ZX09103029) 天津市科技支撐重點(diǎn)項(xiàng)目(09ZCKFSH00100)
【分類號(hào)】：R749.16
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本文編號(hào)：2430468